Highly efficient imidazolium-containing oligomers for preventing MRSA biofilm and postoperative spinal infection

Li, Jiangbi; Wang, Yongjie; Che, Mingxue; Ji, Xue; Li, Jun; Sun, Yansong; Xiao, Chunsheng; Zhang, Yugen; Zhang, Shaokun

doi:10.1016/j.eurpolymj.2020.109910

Cited by 4 publications

(3 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The L‐shaped stainless steel implant (0.1 mm in diameter) was then placed into the defect. The long arm of the implant was longitudinally placed along the spine heading cranially with the short arm in the spine, as shown in our previous study 54 . An inoculation of sterile saline or 1 × 10 2 , 1 × 10 3 , or 1 × 10 4 CFUs of bioluminescent Xen36 S. aureus was applied to the 90° bend of the stainless steel.…”

Section: Mouse Modelsmentioning

confidence: 99%

Animal Models for Postoperative Implant‐Related Spinal Infection

Wang

Che

Zheng

et al. 2022

Orthopaedic Surgery

Self Cite

View full text Add to dashboard Cite

Postoperative infections following implant‐related spinal surgery are severe and disastrous complications for both orthopaedic surgeons and patients worldwide. They can cause neurological damage, disability, and death. To better understand the mechanism of these destructive complications and intervene in the process, further research is needed. Therefore, there is an urgent need for efficient, accurate, and easily available animal models to study the pathogenesis of spinal infections and develop new and effective anti‐bacterial methods. In this paper, we provide a general review of the commonly used animal models of postoperative implant‐related spinal infections, describe their advantages and disadvantages, and highlight the significance of correctly choosing the model according to the infection aspect under investigation. These models are valuable tools contributing to the better understanding of postoperative spinal infections and will continue to facilitate the invention of novel preventative and treatment strategies for patients with postoperative spinal infections. However, although they are valid and reproducible in some respects, the current animal models present certain limitations. Future ideal spinal infection animal models may assess the bacterial load of the same animal in real‐time in vivo, and better mimic the human anatomy as well as surgical techniques. Strains other than Staphylococcus aureus account for a large proportion of postoperative spinal infections, and thus, the establishment of models to evaluate other types of microbial infections is expected in the future. Furthermore, novel transgenic models established on advancements in genome editing are also likely to be developed in the future.

show abstract

Section: Mouse Modelsmentioning

confidence: 99%

Animal Models for Postoperative Implant‐Related Spinal Infection

Wang

Che

Zheng

et al. 2022

Orthopaedic Surgery

Self Cite

View full text Add to dashboard Cite

show abstract

“…8,9 Due to the robust self-secreted extracellular polymeric substance (EPS), bacterial biofilms are usually 100−1000 times more tolerant to antibiotics than corresponding planktonic cells. 10 As a result, there arises an urgent need for new antimicrobials with potent therapeutic activity to combat the growing epidemic of infections caused by Gramnegative pathogens and the surfaced-adhered biofilms.…”

Section: Introductionmentioning

confidence: 99%

“…Various efforts have then been greatly devoted to developing new antibiotics . However, of serious concern is the fact that no new class of antibiotics has been approved for the treatment of Gram-negative bacteria infections in over 50 years. , The primary challenge lies in the treatment of Gram-negative bacteria mediated by the presence of dual membranes, which acts as an impermeable barrier to most hydrophobic antibiotics, such as rifamycins. , On top of that, the formation of bacterial biofilms has further worsened the current scenario. , Due to the robust self-secreted extracellular polymeric substance (EPS), bacterial biofilms are usually 100–1000 times more tolerant to antibiotics than corresponding planktonic cells . As a result, there arises an urgent need for new antimicrobials with potent therapeutic activity to combat the growing epidemic of infections caused by Gram-negative pathogens and the surfaced-adhered biofilms.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Synergistic Antibacterial and Antibiofilm Efficacy of Main-Chain Polysulfoniums with Antibiotics by Balancing Charge Density and Amphiphilicity

Zhao

Wang

et al. 2023

ACS Appl. Polym. Mater.

View full text Add to dashboard Cite

Infection diseases caused by Gram-negative pathogens are exceedingly difficult to treat because their characteristic outer membrane limits antibiotic entry. Herein, we report the development of main-chain polysulfoniums with different charge densities and amphiphilicities based on the quantitative methylation reaction. By regulating the membrane-disruption capability, the combined efficacy of polysulfoniums and antibiotic rifampicin can be manipulated from no interaction to synergy against Gram-negative bacteria Escherichia coli. After incubating with synergistic polysulfoniums/rifampicin combinations at a minimum inhibitory concentration (MIC), the time needed to achieve a 6-log reduction of E. coli can be accelerated 8 times compared to the antibiotic treatment. At 1/2 MIC, polysulfoniums/rifampicin combinations can provide a 90% reduction in biofilm mass and 8-log orders of embedded bacteria killing in 3-day-mature E. coli biofilms. This work demonstrates that alkylation chemistry can serve as a reliable means to create antibiotic adjuvants in combating infections caused by Gram-negative pathogens and biofilms.

show abstract