Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure

Ota, Shuhei; Oshima, Koichiro; Yamazaki, T.; Kim, Sangwan; Yu, Zhe; Yoshihara, Mai; Takeda, Kohei; Takeshita, Tsuyoshi; Hirata, Aiko; Bišová, Kateřina; Zachleder, Vilém; Hattori, Masahira; Kawano, Shigeyuki

doi:10.1186/s13068-016-0424-2

Cited by 63 publications

(36 citation statements)

References 50 publications

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“…Subsequently, the KEGG map was manually redrawn using Adobe Illustrator CC 2014 (Adobe Systems). Heat maps of the expressed genes involved in surfactin biosynthesis were generated using the heatmap.2 function from the Gplots package in R statistical software v. 3.1.069. Statistical testing for gene expression was performed in R with DESeq using the no replicate method (Supplementary dataset 8)66.…”

Section: Methodsmentioning

confidence: 99%

Genome and transcriptome analysis of surfactin biosynthesis in Bacillus amyloliquefaciens MT45

Zhi

2017

Sci Rep

106

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Natural Bacillus isolates generate limited amounts of surfactin (<10% of their biomass), which functions as an antibiotic or signalling molecule in inter-/intra-specific interactions. However, overproduction of surfactin in Bacillus amyloliquefaciens MT45 was observed at a titre of 2.93 g/l, which is equivalent to half of the maximum biomass. To systemically unravel this efficient biosynthetic process, the genome and transcriptome of this bacterium were compared with those of B. amyloliquefaciens type strain DSM7T. MT45 possesses a smaller genome while containing more unique transporters and resistance-associated genes. Comparative transcriptome analysis revealed notable enrichment of the surfactin synthesis pathway in MT45, including central carbon metabolism and fatty acid biosynthesis to provide sufficient quantities of building precursors. Most importantly, the modular surfactin synthase overexpressed (9 to 49-fold) in MT45 compared to DSM7T suggested efficient surfactin assembly and resulted in the overproduction of surfactin. Furthermore, based on the expression trends observed in the transcriptome, there are multiple potential regulatory genes mediating the expression of surfactin synthase. Thus, the results of the present study provide new insights regarding the synthesis and regulation of surfactin in high-producing strain and enrich the genomic and transcriptomic resources available for B. amyloliquefaciens.

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Section: Methodsmentioning

confidence: 99%

Genome and transcriptome analysis of surfactin biosynthesis in Bacillus amyloliquefaciens MT45

Zhi

2017

Sci Rep

106

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“…Microalgae have received global attention as a promising sustainable feedstock for biofuel production as they use solar energy and convert it to lipids in the form of Triacylglycerols (TAGs) through the process of photosynthesis (Gusbeth et al 2016;Ota et al 2016;Zhu et al 2016;Yu et al 2011). Compared to other feedstocks, microalgae exhibit many advantages such as high photosynthetic efficiency, high growth rate, short doubling time and high lipid productivity (Meng et al 2009;Widjaja et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Imaging the accumulated intracellular microalgal lipids as a response to temperature stress

et al. 2017

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Over the last few decades, many scientists considered microalgae as promising actors for future biofuels because of the high lipid productivity inside their cells. Moreover, much attention has been paid to algal lipids as they can be used in biodiesel production. In this study, we optimized the different suitable conditions such as incubation time, incubation temperature, Dimethylesulfoxide and Nile red concentrations of the lipophilic fluorescence dye Nile red as an excellent and fast vital stain to detect and quantify intracellular lipids. This was achieved using the green alga Nannochloropsis salina. In addition, investigating the accumulation of lipid vesicles inside different isolated microalgal species as a response to temperature stress. Furthermore, the confocal laser scanning microscopy (LS510) for imaging and measuring the size and volume of the accumulated lipid vesicles was used.

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“…On the other hand, the total carbohydrates concentration of A. platensis under sulfur limitation was 20.969 ± 5.192 mg L −1 which was lower than control (24.107 ± 3.555 mg L −1 ). Some studies reported that sulfur limitation triggers lipids synthesis in algal cells . Furthermore, Cakmak et al suggested that there could be a potential competition between lipid and starch/carbohydrate synthesis mechanism whereas Wang et al revealed that lipid and starch syntheses do antagonize each other.…”

Section: Nutrient Limitationmentioning

confidence: 99%

Nutritional optimization of Arthrospira platensis for starch and Total carbohydrates production

Lai

Puspanadan

Lee

2019

Biotechnology Progress

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Present study aims to optimize the production of starch and total carbohydrates from Arthrospira platensis. Growing concerns toward unprecedented environmental issues associated with plastic pollution has created a tremendous impetus to develop new biomaterials for the production of bioplastic. Starch-based biopolymers from algae serve as sustainable feedstock for thermoplastic starch production due to their abundant availability and low cost. A. platensis was cultivated in Zarrouk's medium at 32 ± 1 C and exposed to red light with a photoperiod of 12:12 hr light/dark. Growth kinetics studies showed that the maximum specific growth rate (μ max ) obtained was 0.059 day −1 with the doubling time (t d ) of 11.748 days. Subsequently, Zarrouk's medium with different concentrations of sulfur, phosphorus and nitrogen was prepared to establish the nutrient-limiting conditions to enhance the accumulation of starch and total carbohydrates. In this study, the highest starch accumulated was 6.406 ± 0.622 mg L −1 under optimized phosphorus limitation (0.025 g L −1 ) conditions. Nitrogen limitation (0.250 g L −1 ) results demonstrated significant influenced (p < 0.05) on total carbohydrates (67.573 ± 2.893 mg L −1 ) accumulation in A. platensis. The starch accumulation in A. platensis was significantly affected (p < 0.05) by phosphorus limitation (0.0025 g L −1 ). Subsequently, the optimized phosphorus concentration was coupled with mixotrophic cultivation to further enhance the starch accumulation. The results obtained indicated that, the starch (11.426 ± 0.314 mg L −1 ) and carbohydrates (43.053 ± 2.986 mg L −1 ) concentration obtained was significantly high (p < 0.05) under mixotrophic cultivation. Therefore, it shown that nutrient limitation and mixotrophic cultivation are viable strategies to enhance the accumulation of starch and total carbohydrates in A. platensis. K E Y W O R D SArthrospira platensis, mixotrophic, nutrient limitation, starch and carbohydrates

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Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure

Cited by 63 publications

References 50 publications

Genome and transcriptome analysis of surfactin biosynthesis in Bacillus amyloliquefaciens MT45

Genome and transcriptome analysis of surfactin biosynthesis in Bacillus amyloliquefaciens MT45

Imaging the accumulated intracellular microalgal lipids as a response to temperature stress

Nutritional optimization of Arthrospira platensis for starch and Total carbohydrates production

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