Highly efficient rapid micropropagation and assessment of genetic fidelity of regenerants by ISSR and SCoT markers of Solanum khasianum Clarke

Chirumamilla, Pavani; Gopu, Chaitanya; Jogam, Phanikanth; Taduri, Shasthree

doi:10.1007/s11240-020-01964-6

Cited by 46 publications

(17 citation statements)

References 38 publications

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“…All media combinations of BAP with 2,4-D had very high callus induction responses for both leaf and root explants with the best being a combination of 1.0 mg/L BAP and 1.0 mg/L 2,4-D for leaf explants and 0.5 mg/L BAP with 1.0 mg/L 2,4-D for root explants. Similar observations have also been made in many studies on various species such as Solanum khasianum (Chirumamilla et al, 2021), Gynura procumbens (Nurokhman et al, 2019), Trachyspermum ammi (Fazeli-Nasab, 2018), Tridax procumbens (Wani et al, 2010), Stevia rebaudiana (Ahmad et al, 2011), and Chrysanthemum morifolium (Khan et al, 2020).…”

Section: Discussionsupporting

confidence: 78%

“…We observed that the rate of callus induction from both leaf and root explants was significantly higher (p < 0.05) in media supplemented with the combinations of cytokinins and auxins than in media containing either cytokinins or auxins alone. Similar observations have been made for many other plant species in the same family as A. africana, such as Elephantopus scaber (Abraham and Thomas, 2015), Centaurea arifolia (Yüzbaşioglu et al, 2012), Stevia rebaudiana (Gupta et al, 2010;Narender et al, 2011;El-Zaidy et al, 2014), Senecio candicans (Hariprasath et al, 2015), and Pentanema indicum (Sivanesan and Jeong, 2007), indicating a synergistic effect of cytokinin-auxin interaction in callus induction (Chirumamilla et al, 2021).…”

Section: Discussionsupporting

confidence: 64%

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Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

et al. 2021

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The medicinal plant, Aspilia africana, has been traditionally used in several African countries to treat many diseases such as tuberculosis, cough, inflammation, malaria, osteoporosis, and diabetes. In this study, we developed a protocol for in vitro propagation of A. africana using indirect shoot organogenesis from leaf and root explants of in vitro-grown seedlings and assessed the tissues at different developmental stages. The highest callus induction (91.9 ± 2.96%) from leaf explants was in the Murashige and Skoog (MS) medium augmented with 1.0 mg/L 6-Benzylaminopurine (BAP) and 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) while from root explants, the highest callus induction (92.6 ± 2.80%) was in the same plant tissue culture medium augmented with 0.5 mg/L BAP and 1.0 mg/L 2,4-D. The best shoot regeneration capacity from leaf-derived calli (i.e., 80.0 ± 6.23% regeneration percentage and 12.0 ± 6.23 shoots per callus) was obtained in medium augmented with 1.0 mg/L BAP and 0.05 mg/L α-Naphthaleneacetic acid (NAA); the best regeneration capacity for root-derived calli (i.e., 86.7 ± 6.24% shoot regeneration percentage and 14.7 ± 1.11 shoots per callus) was obtained in the MS medium augmented with 1.0 mg/L BAP, 0.05 mg/L NAA, and 0.1 mg/L Thidiazuron (TDZ). Regenerated plantlets developed a robust root system in 1/2 MS medium augmented with 0.1 mg/L NAA and had a survival rate of 93.6% at acclimatization. The in vitro regenerated stem tissue was fully differentiated, while the young leaf tissue consisted of largely unorganized and poorly differentiated cells with large intercellular airspaces typical of in vitro leaf tissues. Our study established a protocol for the indirect regeneration of A. africana and offers a basis for its domestication, large-scale multiplication, and germplasm preservation. To the best of our knowledge, this is the first study to develop an indirect regeneration protocol for A. africana and conduct anatomical assessment through the different stages of development from callus to a fully developed plantlet.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionsupporting

confidence: 64%

Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

et al. 2021

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“…Solanum khasianum is a traditional medicinal plant belonging to Solanaceae family. The plant was known to possess potential alkaloids (solasodine, solasonine, solanine, solamargine and khasianine) that represent an alternative source of medicine (Kaunda and Zhang 2019 ; Chirumamilla et al 2021 ). The berries of S. khasianum was reported to possess anticancer (Rosangkima and Jagetia 2015 ), antibacterial (Pavani and Shasthree 2021 ), anti-inflammatory (Chirumamilla et al 2022 ), antioxidant, anti-diabetic and anti-cholinesterase properties (Gogoi et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

GC–MS profiling and antibacterial activity of Solanum khasianum leaf and root extracts

2022

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Background Solanum khasianum is an important medicinal herb of the Solanaceae family. The present study was focused to determine the bioactive compounds in S. khasianum leaf and root extract by GC–MS analysis and their antibacterial activity by agar well diffusion method. Results Sixteen bioactive compounds were detected in leaf extract and thirty-two compounds in root methanolic extract by GC–MS. The major potent compounds identified in leaf and root extracts were heptadecane 9-hexyl (43.65%) and stigmasterol (23.18%). The root extract showed increased antibacterial activity than leaf extract. Conclusion These extracts possessed significant antibacterial activity against the tested bacterial isolates in dose-dependent manner. This study provides the phytoconstituents, antibacterial property and scientific evidence for the traditional claim and use of S. khasianum.

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“…Solanaceae is one such family with different biological activities. S.khasianum, belonging to the family Solanaceae is known for its varied medicinal properties [4]. Aforesaid kind of investigations has increased drastically during recent years, signifying the necessity and importance of plant-based antimicrobial compounds.…”

Section: Introductionmentioning

confidence: 99%

Biological activity of green synthesized silver nanoparticles and different plant extracts of Solanum khasianum Clarke

Chirumamilla¹,

Taduri²

2021

IRJASH

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Solanum khasianum Clarke. (Solanaceae), an important medicinal plant used to treat several diseases. Leaf, root, fruit and bark extracts of S. khasianum and the green synthesized silver nanoparticles were assayed for its antibacterial activity against gram positive and gram negative bacteria, using agar well diffusion method. Among all the solvent extracts tested, methanolic extracts were found to possess elevated activity. The optimal antibacterial activity is exhibited by silver nanoparticles against Pseudomonas flourescens with a maximal zone of inhibition (22mm). In the present study, the efficacy of green synthesized silver nanoparticles over the different plant extracts of S. khasianum against different bacterial strains was reported. This investigation discloses the potentiality of using silver nanoparticles as an antibacterial agent.

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Highly efficient rapid micropropagation and assessment of genetic fidelity of regenerants by ISSR and SCoT markers of Solanum khasianum Clarke

Cited by 46 publications

References 38 publications

Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

GC–MS profiling and antibacterial activity of Solanum khasianum leaf and root extracts

Biological activity of green synthesized silver nanoparticles and different plant extracts of Solanum khasianum Clarke

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