Highly enriched anammox within anoxic biofilms by reducing suspended sludge biomass in a real-sewage A2/O process

Li, Jianwei; Peng, Yongzhen; Gao, Ruitao; Liu, Yang; Deng, Liyan; Zhao, Qi; Liu, Qiyu; Li, Xiyao; Zhang, Qiong; Liang, Zhen

doi:10.1016/j.watres.2021.116906

Cited by 121 publications

(10 citation statements)

References 60 publications

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“…Some studies have recently revealed that the A-biofilms are the ideal location for retaining anammox bacteria in a continuous-flow nitrogen removal process, as anoxic environments can promote anammox enrichment which obtain nitrite from partial denitrification ( Li et al., 2021 ; Li et al., 2019 ). Herein, we examined whether nitrate-dependent denitrification with HRAS effluent could support the A-biofilm anammox, i.e., partial denitrification and anammox (PD/A).…”

Section: Resultsmentioning

confidence: 99%

One-year stable pilot-scale operation demonstrates high flexibility of mainstream anammox application

Zheng

Duan

et al. 2023

Water Research X

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Section: Resultsmentioning

confidence: 99%

One-year stable pilot-scale operation demonstrates high flexibility of mainstream anammox application

Zheng

Duan

et al. 2023

Water Research X

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“…Here, the proposed nitrogen removal A/O system can be upgraded from the current A/A/O system without significant capital costs. On the one hand, anoxic biofilms that were added to the A zone have been achievable at full scale, reported recently. − On the other hand, the added unit for iron dosing only has a volume of 7.5% of the anaerobic zone in the conventional A/A/O process, meaning that it can be directly re-fitted. Moreover, despite dosing into the return sludge, the average iron dosage in terms of treated wastewater was calculated to be 20 mg Fe/L, which is close to the typical range of iron salt dosed (10–40 mg Fe/L-treated wastewater) in many WWTPs. ,, Doing of FeCl 3 slightly reduced the pH of wastewater (Figure S2), which indicates that the alkalinity within wastewater is sufficient to buffer the proton produced in the Fe 3+ hydrolytic process.…”

Section: Resultsmentioning

confidence: 99%

Novel Use of a Ferric Salt to Enhance Mainstream Nitrogen Removal from Anaerobically Pretreated Wastewater

et al. 2023

Environ. Sci. Technol.

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This study aims to demonstrate a new technology roadmap to support the ongoing paradigm shift in wastewater management from pollutant removal to resource recovery. This is achieved by developing a novel use of an iron salt (i.e., FeCl 3 ) in an integrated anaerobic wastewater treatment and mainstream anammox process. FeCl 3 was chosen to be dosed in a proposed sidestream unit rather than in a primary settler or a mainstream reactor. This causes acidification of returned activated sludge and enables stable suppression of nitrite-oxidizing bacterial activity and excess sludge reduction. A laboratory-scale system, which comprised an anaerobic baffled reactor, a continuous-flow anoxic−aerobic (A/O) reactor, and a secondary settler, was designed to treat real domestic wastewater, with the performance of the system comprehensively monitored under a steady-state condition. The experimental assessments showed that the system had good effluent quality, with total nitrogen and phosphorus concentrations of 12.6 ± 1.3 mg N/L and 0.34 ± 0.05 mg P/L, respectively. It efficiently retained phosphorus in excess sludge (0.18 ± 0.03 g P/g dry sludge), suggesting its potential for further recovery. About half of influent organic carbon was recovered in the form of bioenergy (i.e., methane). This together with low energy consumption revealed that the system could produce a net energy of about 0.11 kWh/m 3 -wastewater, assessed by an energy balance analysis.

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“…Biomass segregation with nitrifying bacteria, HB, and AnAOB has been demonstrated in a mathematical modeling study of PN/anammox . Under biomass granular/floc segregation conditions, the PAOs predominantly resided in the flocs, and the low floc SRTs led to an effective selective discharge or an insufficient number of flocs that ensured the potential dynamic balance of PAOs and AnAOB and the stability of the anammox system compared to a no biomass segregation (granules alone) system. ,, …”

Section: Discussionmentioning

confidence: 99%

“…34 Under biomass granular/floc segregation conditions, the PAOs predominantly resided in the flocs, and the low floc SRTs led to an effective selective discharge or an insufficient number of flocs that ensured the potential dynamic balance of PAOs and AnAOB and the stability of the anammox system compared to a no biomass segregation (granules alone) system. 20,35,36 4.3. Possible Applications of Directly Integrating Anammox into Sewage Treatment.…”

Section: Enhancing Two Nitrite Pathways For Anammoxmentioning

confidence: 99%

Successful Application of Anammox Using the Hybrid Autotrophic–Heterotrophic Denitrification Process for Low-Strength Wastewater Treatment

Peng

Yang

et al. 2022

Environ. Sci. Technol.

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Directly integrating anammox into sewage treatment is attractive, but anammox bacteria (AnAOB) enrichment is complex due to vicious competition from heterotrophic bacteria (HB). A novel strategy of optimal organics management using a preanaerobic stage and subsequent limited-oxygen conditions (0.32 ± 0.15 mg-O 2 /L) is applied, and a hybrid autotrophic− heterotrophic denitrification process is developed to treat sewagelike wastewater with a COD/N ratio of 3.1 for 420 days. The stable process was achieved, and a high total nitrogen removal rate of 0.53 kg-N/(m 3 •d) was obtained compared to conventional nitrification/denitrification. The 16S rRNA high-throughput sequencing analysis suggested that the relative abundance of the nonendogenous HB (Denitratisoma and Thauera) was drastically reduced (P ≤ 0.001), whereas the endogenous denitrifying HB (Candidatus (Ca.) Competibacter) was significantly enriched in the anammox granules (9.98%, P ≤ 0.001). Moreover, Ca. Competibacter as an inner core and Nitrospira and Ca. Brocadia as an outside coating of the anammox granules indicated the cooperation of AnAOB with HB as revealed by laser-scanning confocal microscopy and qPCR. In situ tests further confirmed nitrite from two pathways (partial nitritation and endogenous partial denitritation) that favored AnAOB enrichment. Optimal organics management can mitigate the competition of HB with AnAOB by redirecting the metabolic pathways and microbial community, which is critical to directly integrating anammox into sewage treatment.

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Highly enriched anammox within anoxic biofilms by reducing suspended sludge biomass in a real-sewage A2/O process

Cited by 121 publications

References 60 publications

One-year stable pilot-scale operation demonstrates high flexibility of mainstream anammox application

One-year stable pilot-scale operation demonstrates high flexibility of mainstream anammox application

Novel Use of a Ferric Salt to Enhance Mainstream Nitrogen Removal from Anaerobically Pretreated Wastewater

Successful Application of Anammox Using the Hybrid Autotrophic–Heterotrophic Denitrification Process for Low-Strength Wastewater Treatment

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