Highly permselective uric acid detection using kidney cell membrane–functionalized enzymatic biosensors

Kim, Insu; Kim, Young Im; Lee, Sang Won; Jung, Hyo Gi; Lee, Gyudo; Yoon, Dae Sung

doi:10.1016/j.bios.2021.113411

Cited by 27 publications

(8 citation statements)

References 23 publications

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“…By employed URAT1-contained membrane in a sensor, Kim et al developed a KCM-coated UA biosensor (called the KCM sensor) that could selectively detect uric acid through URAT1. 148 The KCM sensor enabled the detection of uric acid in the range of 0−1000 μM, with a limit of detection of 8.5 μM, suggesting that it allows the diagnosis of the early stages of gout. Studies also showed that the transient receptor potential vanilloid 1 (TRPV1) is a key target for spicy taste sensor and analgesic drug development.…”

Section: ■ Application Of Extracted Cell Membrane For Biosensingmentioning

confidence: 99%

Engineering Cell Membranes: From Extraction Strategies to Emerging Biosensing Applications

Tan,

Zhu,

et al. 2024

Anal. Chem.

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Section: ■ Application Of Extracted Cell Membrane For Biosensingmentioning

confidence: 99%

Engineering Cell Membranes: From Extraction Strategies to Emerging Biosensing Applications

Tan,

Zhu,

et al. 2024

Anal. Chem.

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Section: Antibiofouling Propertymentioning

confidence: 99%

“…UA derives from the final metabolism of purine, which is a constituent of nucleic acids, and is finally excreted into the urine by the kidney [49]. Evaluated serum UA level (also called hyperuricemia) could lead to gout and nephrolithiasis, which has recently been found to be related to hypertension, coronary heart disease, heart failure, atrial fibrillation, insulin resistance, and nonalcoholic fatty liver disease [50].…”

Section: Antibiofouling Propertymentioning

confidence: 99%

A Micro Electrochemical Sensor for Multi-Analyte Detection Based on Oxygenated Graphene Modified Screen-Printed Electrode

Yuan

Gan

et al. 2022

Nanomaterials

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Electrode interfaces with both antibiofouling properties and electrocatalytic activity can promote the practical application of nonenzymatic electrochemical sensors in biological fluids. Compared with graphene, graphene oxide (GO) possesses unique properties such as superior solubility (hydrophilicity) in water, negative charge, and abundant oxygenated groups (oxo functionalities) in the plane and edge sites, which play an essential role in electrocatalysis and functionalization. In this work, a micro electrochemical sensor consisting of GO-modified screen-printed electrode and PDMS micro-cell was designed to achieve multi-analyte detection with excellent selectivity and anti-biofouling properties by electrochemically tuning the oxygen-containing functional species, hydrophilicity/hydrophobicity, and electrical conductivity. In particular, the presented electrodes demonstrated the potential in the analysis of biological samples in which electrodes often suffer from serious biofouling. The interaction of proteins with electrodes as well as uric acid was investigated and discussed.

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“…EM was extracted from whole human blood and functionalized with a single layer on an MC. The surface functionalization of EM on the MC has two advantages over conventional receptor functionalization on the MC [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 ]. First, the cumbersome process of receptor immobilization can be omitted.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, no additional molecular passivation is required between the receptors [ 39 ]. Recently, the EM functionalization of nanoparticles [ 53 , 54 ], electrodes [ 55 , 56 , 57 , 58 , 59 ], and paper sensors [ 60 ] has conferred highly sensitive and selective ligand–receptor binding, as well as the sophisticated filtration of interfering molecules. In this study, we demonstrated the detection performance of an EMMC as a function of IgM concentration ( Figure 1 c).…”

Section: Introductionmentioning

confidence: 99%

Hemolysis-Inspired, Highly Sensitive, Label-Free IgM Detection Using Erythrocyte Membrane-Functionalized Nanomechanical Resonators

et al. 2022

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Immunoglobulin detection is important for immunoassays, such as diagnosing infectious diseases, evaluating immune status, and determining neutralizing antibody concentrations. However, since most immunoassays rely on labeling methods, there are limitations on determining the limit of detection (LOD) of biosensors. In addition, although the antigen must be immobilized via complex chemical treatment, it is difficult to precisely control the immobilization concentration. This reduces the reproducibility of the biosensor. In this study, we propose a label-free method for antibody detection using microcantilever-based nanomechanical resonators functionalized with erythrocyte membrane (EM). This label-free method focuses on the phenomenon of antibody binding to oligosaccharides (blood type antigen) on the surface of the erythrocyte. We established a method for extracting the EM from erythrocytes and fabricated an EM-functionalized microcantilever (MC), termed EMMC, by surface-coating EM layers on the MC. When the EMMC was treated with immunoglobulin M (IgM), the bioassay was successfully performed in the linear range from 2.2 pM to 22 nM, and the LOD was 2.0 pM. The EMMC also exhibited excellent selectivity compared to other biomolecules such as serum albumin, γ-globulin, and IgM with different paratopes. These results demonstrate that EMMC-based nanotechnology may be utilized in criminal investigations to identify blood types with minimal amounts of blood or to evaluate individual immunity through virus-neutralizing antibody detection.

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Highly permselective uric acid detection using kidney cell membrane–functionalized enzymatic biosensors

Cited by 27 publications

References 23 publications

Engineering Cell Membranes: From Extraction Strategies to Emerging Biosensing Applications

Engineering Cell Membranes: From Extraction Strategies to Emerging Biosensing Applications

A Micro Electrochemical Sensor for Multi-Analyte Detection Based on Oxygenated Graphene Modified Screen-Printed Electrode

Hemolysis-Inspired, Highly Sensitive, Label-Free IgM Detection Using Erythrocyte Membrane-Functionalized Nanomechanical Resonators

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