Highly Photostable Carbon Dots from Citric Acid for Bioimaging

Fiori, Federico; Moukham, Hind; Olia, Federico; Ledda, Sergio; Salis, Andrea; Stagi, Luigi; Malfatti, Luca; Innocenzi, Plinio

doi:10.3390/ma15072395

Cited by 11 publications

(3 citation statements)

References 31 publications

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“…Fluorescence stability refers to the stability of the fluorescence emission of 0D CNMs during the long-term luminescence operation, in which the intensity gradually weakens or disappears over time and through interference from other substances. , Because they easily aggregate into large nanocrystals, traditional 0D CNMs exhibit poor photostability. The temperature, solvent, pH, polarity, oxidants, and metal ions are the most common conditions that affect the in vitro photostability of 0D CNMs fluorescence properties .…”

Section: Optical Properties Of 0d Cnmsmentioning

confidence: 99%

Zero-Dimensional Carbon Nanomaterials for Fluorescent Sensing and Imaging

Yang,

Xu,

et al. 2023

Chem. Rev.

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Advances in nanotechnology and nanomaterials have attracted considerable interest and play key roles in scientific innovations in diverse fields. In particular, increased attention has been focused on carbon-based nanomaterials exhibiting diverse extended structures and unique properties. Among these materials, zero-dimensional structures, including fullerenes, carbon nano-onions, carbon nanodiamonds, and carbon dots, possess excellent bioaffinities and superior fluorescence properties that make these structures suitable for application to environmental and biological sensing, imaging, and therapeutics. This review provides a systematic overview of the classification and structural properties, design principles and preparation methods, and optical properties and sensing applications of zero-dimensional carbon nanomaterials. Recent interesting breakthroughs in the sensitive and selective sensing and imaging of heavy metal pollutants, hazardous substances, and bioactive molecules as well as applications in information encryption, super-resolution and photoacoustic imaging, and phototherapy and nanomedicine delivery are the main focus of this review. Finally, future challenges and prospects of these materials are highlighted and envisaged. This review presents a comprehensive basis and directions for designing, developing, and applying fascinating fluorescent sensors fabricated based on zero-dimensional carbon nanomaterials for specific requirements in numerous research fields.

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Section: Optical Properties Of 0d Cnmsmentioning

confidence: 99%

Zero-Dimensional Carbon Nanomaterials for Fluorescent Sensing and Imaging

Yang,

Xu,

et al. 2023

Chem. Rev.

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“…Notably, the exceptional optical properties and high specific surface area of CDs allow for surface physical and chemical modifications, further enhancing their functionality in biomedical applications [14][15][16][17][18]. A highly efficient protocol has recently been developed to yield decagram-scale quantities of N,S-doped CDs featuring a narrow size distribution and bright emission.…”

Section: Introductionmentioning

confidence: 99%

Microporous Fluorescent Poly(D,L-lactide) Acid–Carbon Nanodot Scaffolds for Bone Tissue Engineering Applications

Mauro,

Calabrese,

Sciortino

et al. 2024

Materials

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In this study, we introduce novel microporous poly(D,L-lactide) acid–carbon nanodot (PLA-CD) nanocomposite scaffolds tailored for potential applications in image-guided bone regeneration. Our primary objective was to investigate concentration-dependent structural variations and their relevance to cell growth, crucial aspects in bone regeneration. The methods employed included comprehensive characterization techniques such as DSC/TGA, FTIR, rheological, and degradation assessments, providing insights into the scaffolds’ thermoplastic behavior, microstructure, and stability over time. Notably, the PLA-CD scaffolds exhibited distinct self-fluorescence, which persisted after 21 days of incubation, allowing detailed visualization in various multicolor modalities. Biocompatibility assessments were conducted by analyzing human adipose-derived stem cell (hADSC) growth on PLA-CD scaffolds, with results substantiated through cell viability and morphological analyses. hADSCs reached a cell viability of 125% and penetrated throughout the scaffold after 21 days of incubation. These findings underscore the scaffolds’ potential in bone regeneration and fluorescence imaging. The multifunctional nature of the PLA-CD nanocomposite, integrating diagnostic capabilities with tunable properties, positions it as a promising candidate for advancing bone tissue engineering. Our study not only highlights key aspects of the investigation but also underscores the scaffolds’ specific application in bone regeneration, providing a foundation for further research and optimization in this critical biomedical field.

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“…Despite their potential, there is a scarcity of research on optimizing the synthetic yield and processes of CDs. CDs can be synthesized from various sources such as organic small molecules, − macromolecules, , and natural biomass materials. − However, the reported synthetic yields in the literature remain low, which restricts their development and large-scale production. Orange juice has been employed as a precursor for CDs synthesis via one-step hydrothermal treatment at a relatively low temperature (120 °C) and short reaction time (150 min) .…”

Section: Introductionmentioning

confidence: 99%

EGTA-Derived Carbon Dots with Bone-Targeting Ability: Target-Oriented Synthesis and Calcium Affinity

Liu,

Li,

et al. 2023

ACS Appl. Mater. Interfaces

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The bone-targeting mechanism of clinic bisphosphonate-type drugs, such as alendronate, risedronate, and ibandronate, relies on chelated calcium ions on the surface of the bone mineralized matrix for the treatment of osteoporosis. EGTA with aminocarboxyl chelating ligands can specifically chelate calcium ions. Inspired by the bone-targeting mechanism of bisphosphonates, we hypothesize that EGTA-derived carbon dots (EGTA-CDs) hold bone-targeting ability. For the target-oriented synthesis of EGTA-CDs and to endow CDs with bone targeting, we designed calcium ion chelating agents as precursors, including aminocarboxyl chelating agents (EGTA and EDTA) and bisphosphonate agents (ALN and HEDP) for the target-oriented synthesis of aminocarboxyl-derived CDs (EGTA-CDs and EDTA-CDs) and bisphosphonate-derived CDs (ALN-CDs and HEDP-CDs) with high synthetic yield. The synthetic yield of EGTA-CDs reached 87.6%. Aminocarboxyl-derived CDs and bisphosphonate-derived CDs retain the chelation ability of calcium ions and can specifically bind calcium ions. The chemical environment bone-targeting value coordination constant K and chelation sites of EGTA-CDs were 6.48 × 10 4 M −1 and 4.12, respectively. A novel method was established to demonstrate the bone-targeting capability of chelate-functionalized carbon dots using fluorescence quenching in a simulated bone trauma microenvironment. EGTA-CDs exhibit superior bone-targeting ability compared with other aminocarboxylderived CDs and bisphosphonate-derived CDs. EGTA-CDs display exceptional specificity toward calcium ions and better bone affinity than ALN-CDs, suggesting their potential as novel bone-targeting drugs. EGTA-CDs with strong calcium ion chelating ability have calcium ion affinity in simulated body fluid and bone-targeting ability in a simulated bone trauma microenvironment. These findings offer new avenues for the development of advanced bone-targeting strategies.

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Highly Photostable Carbon Dots from Citric Acid for Bioimaging

Cited by 11 publications

References 31 publications

Zero-Dimensional Carbon Nanomaterials for Fluorescent Sensing and Imaging

Zero-Dimensional Carbon Nanomaterials for Fluorescent Sensing and Imaging

Microporous Fluorescent Poly(D,L-lactide) Acid–Carbon Nanodot Scaffolds for Bone Tissue Engineering Applications

EGTA-Derived Carbon Dots with Bone-Targeting Ability: Target-Oriented Synthesis and Calcium Affinity

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