Highly Reproducible Techniques for Use in Systematic Bacteriology in the Genus Mycobacterium: Tests for Niacin and Catalase and for Resistance to Isoniazid, Thiophene 2-Carboxylic Acid Hydrazide, Hydroxylamine, and p-Nitrobenzoate

Wayne, Lawrence G.; Engel, H. W. B.; Grassi, C; Gross, W.; Hawkins, Jean E.; Jenkins, Peter; Käppler, W; Kleeberg, H. H.; Krasnow, Irving; Nel, E. E.; Pattyn, S. R.; Richards, PA; Showalter, Stephen D.; Slosárek, M; Szabó, István; Tarnok, I; Tsukamura, M; Vergmann, B; Wolinsky, Emanuel

doi:10.1099/00207713-26-3-311

Cited by 56 publications

(35 citation statements)

References 10 publications

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“…Colony morphology and the ability to grow at various temperatures (24,31,37, and 45°C) were determined after 1 week of incubation on Loewenstein-Jensen medium slants. The following properties were determined as described previously: the niacin test was performed by using test strips (Difco Laboratories, Detroit, Mich.) (18,37); nitrate reductase activity was determined by the method of Kubica and David (19); catalase activity at 68°C was determined by the method of Kubica and Pool (20); arylsulfatase activity after 3 days was determined by the method of Kubica and Ridgon (21); p-glucosidase and P-galactosidase activities were determined by the method of Tsukamura (31); Tween 80 hydrolysis was determined by the method of Wayne et al (36); growth on MacConkey agar was determined by the method of Pattyn and Portaels (25); resistance to NaCl and p-nitrobenzoic acid was determined by the methods of Wayne et al (36) and Tsukamura and Tsukamura (34); acetamidase, allantoinase, benzamidase, nicotinamidase, pyrazinamidase, succinamidase, and urease activities were determined by the method of Bonicke (2); and tests to determine resistance to streptomycin Tests to determine decomposition of adenine, guanine, hypoxanthine, xanthine, tyrosine, elastine, keratine, and testosterone were performed by the method of Gordon and Smith (15); tests to determine esculin decomposition were performed by the method of Gordon (13); and tests to determine casein and gelatin hydrolysis were performed by the method of Gordon and Mihm (14). Urea decomposition was tested by using urea agar base (code CM 53; Oxoid) after 2.2% urea was added.…”

Section: Methodsmentioning

confidence: 99%

Tsukamurella inchonensis sp. nov.

Yassin

Rainey²,

Brzezinka³

et al. 1995

International Journal of Systematic Bacteriology

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Chemotaxonomic and genomic 16s ribosomal DNA sequence analyses of two isolates obtained from two different clinical materials clearly delineated a new species of the genus TsukamureZlu. This new species can be identified by its 16s ribosomal DNA similarity values, as well as its physiological characteristics. The name TsukamureZh inchonensis sp. nov. is proposed for these isolates, which are represented by strain IMMIB D-771T (= DSM 44067T) (T = type strain). This strain exhibits only 45% DNA relatedness to Tsukamurella pauro-

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Section: Methodsmentioning

confidence: 99%

Tsukamurella inchonensis sp. nov.

Yassin

Rainey²,

Brzezinka³

et al. 1995

International Journal of Systematic Bacteriology

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“…Degradation of p-aminobenzoate and p-aminosalicylate was tested on Ogawa egg medium (Tsukamura, 1961). Tween hydrolysis (Wayne et aL, 1976), acid phosphatase activity (Stanford & Beck, 1969) using Sigma 104 phosphatase substrate (Sigma Chemical Co.), and catalase activity before and after exposure to 68 °C for 20 rain were also tested. Growth on MacConkey agar (Difco) and reduction of potassium tellurite (Sigma) on BHI agar (Difco) were observed.…”

Section: Characterization Of Strainsmentioning

confidence: 99%

Isolation and characterization of a new subspecies ofMycobacterium chelonei infectious for salmonid fish

Arakawa

Fryer

1984

Helgolander Meeresunters

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Rapidly growing, nonchromogenic mycobacteria were isolated from salmonid fish at five locations in the states of Oregon and Montana, USA. The isolates were characterized by biochemical, physiological, genetic and mycolic acid properties, then subjected to taxonomic analysis. Detection of mycobacterial mycolic acids and a percent guanine plus cytosine value of 63 _+ 1,7 mol% confirmed that the isolates belong to the genus Mycobacterium. The internal similarity of the isolates was 94.2 _+ 3.4 %. None of the isolates grew at 37 °C. A comparison of their properties with those of other rapidly growing, nonchromogenic and photochromogenic mycobacteria was made. The salmonid isolates showed a relationship to/V/, chelonei subspecies chelonei and M, chelonei subspecies abscessus, but had biochemical properties which were intermediate to these two subspecies. Acid methanolysates of the salmonid isolates, analyzed by two dimensional thin-layer chromatography, produced lipid patterns identical to those of both subspecies of M.chelonei. Sufficient differences in biochemical properties and the inability to grow at 37 °C suggest these isolates be regarded as a new subspecies of M. chelonei. We propose the name M. chelonei subspecies piscarium subsp, nov. (L adj. piscariusof fish). The isolates were not infectious for mice.Experimental infections were produced in juvenile salmonid fish. The occurrence of mycobacterial infections in selected salmonid populations from Oregon hatcheries and the Pacific Ocean ranged from 0 to 26 %.

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“…Several mycobacterial proteins such as superoxide dismutase (Kusunose et al, 1980), catalase (Wayne et al, 1976) and other protein antigens such as antigen 5 (Daniel and Janicki, 1978), antigen 21 (Kronvall, Stanford and Walsh, 1976) and antigen 7 of M . leprae (Harboe et al, 1981) have been investigated for polymorphism.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism of a Mycobacterial Antigen Participating in Cell-Mediated Immunity

Hattikudur

Kamat

1985

Journal of Medical Microbiology

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SUMMARY. The cross-reactivity of crude sonic extracts of six species of mycobacteria was studied by skin tests in mice and guinea pigs immunised with live Mycobacterium tuberculosis strain H37Rv, and in patients with pulmonary tuberculosis. All slowly growing mycobacteria elicited a strong delayed hypersensitivity response, M . vaccae a moderate response and M . phlei a poor or no response. A specific target antigen for cell-mediated immunity, known to be present in M . tuberculosis, was also present in all the mycobacteria studied. This shared antigen was shown, by immunoprecipitation tests, to be identical in all the slowly growing species but only a partial reaction of identity was obtained with M . phlei. It is concluded that the antigen shared by the slowly growing mycobacteria is immunodominant in cell-mediated immunity.

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Highly Reproducible Techniques for Use in Systematic Bacteriology in the Genus Mycobacterium: Tests for Niacin and Catalase and for Resistance to Isoniazid, Thiophene 2-Carboxylic Acid Hydrazide, Hydroxylamine, and p-Nitrobenzoate

Cited by 56 publications

References 10 publications

Tsukamurella inchonensis sp. nov.

Tsukamurella inchonensis sp. nov.

Isolation and characterization of a new subspecies ofMycobacterium chelonei infectious for salmonid fish

Polymorphism of a Mycobacterial Antigen Participating in Cell-Mediated Immunity

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