Highly Selective Needle-Type Glucose Sensors Prepared by the Immobilization of Glucose Oxidase on γ-Polyglutamic Acid Film

Yasuzawa, Mikito; Edagawa, Kazuaki; Matsunaga, Takuya; Takaoka, Hiroki; Yabutani, Tomoki

doi:10.2116/analsci.27.337

Cited by 6 publications

(4 citation statements)

References 24 publications

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“…The introduction of permselective inner films, such as cellulose acetate and γ-polyglutamic acid, was essential for practical use. 14 Considering that the obtained CNF aqueous solution was visually stable, we hope that enzyme-CNF solution can be applied as an eco-friendly enzyme ink of ink-jet printing for enzyme sensor fabrication. Such work is now in progress.…”

Section: Fabrication Of Amperometric Glucose Sensor Using Glucose Oximentioning

confidence: 97%

“…Immobilization of the enzyme is one of the most critical processes for the fabrication of an enzyme sensor, since it will highly influence properties of the obtained sensor. Various procedure have been proposed for the preparation of a glucose sensor, such as covalent attachment, [1][2][3] cross-linking, 1,[4][5][6][7][8][9][10][11][12][13][14] hydrogel entrapment, 15,17 polymer film entrapment, 6,[17][18][19][20] and the combination of two or more methods. [21][22][23][24] On the other hand, the concept of green chemistry has infiltrated various fields of the manufacturing industry.…”

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confidence: 99%

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Fabrication of Amperometric Glucose Sensor Using Glucose Oxidase-Cellulose Nanofiber Aqueous Solution

et al. 2015

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Cellulose nanofiber aqueous solution, which remained virtually transparent for more than one week, was prepared by using the clear upper layer of diluted cellulose nanofiber solution produced by wet jet milling. Glucose oxidase (GOx) was easily dissolved in this solution and GOx-immobilized electrode was easily fabricated by simple repetitious drops of GOx-cellulose solution on the surface of a platinum-iridium electrode. Glucose sensor properties of the obtained electrodes were examined in phosphate buffer solution of pH 7.4 at 40°C. The obtained electrode provided a glucose sensor response with significantly high response speed and good linear relationship between glucose concentration and response current. After an initial decrease of response sensitivity for a few days, relatively constant sensitivity was obtained for about 20 days. Nevertheless, the influence of electroactive compounds such as ascorbic acid, uric acid and acetoaminophen were not negletable.

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Section: Fabrication Of Amperometric Glucose Sensor Using Glucose Oximentioning

confidence: 97%

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confidence: 99%

Fabrication of Amperometric Glucose Sensor Using Glucose Oxidase-Cellulose Nanofiber Aqueous Solution

et al. 2015

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“…A highly selective glucose biosensor has been prepared by immobilization of glucose oxidase on a biodegradable g-polyglutamic acid lm. 17 Microtiter plates based on renewable resources, polylactic acid (PLA), have been patented and launched recently. These are reported to be fully biodegradable and possess an antigen binding efficiency equally well or better than conventionally used polystyrene.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of dot-ELISA on modified cellulose filter paper: a simplified novel approach

et al. 2014

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“…[8][9][10][11] With respect to the practical use of enzyme sensors and biofuel cells one of the most important factors is the stable immobilization of all components related to the electron transfer process, from the substrates to the electrode. [15][16][17][18][22][23][24] The electrodes with components modified for electrochemical detection of an enzyme reaction are generally called reagentless electrodes; they work by the addition of only the substrates. In fact, Smolander et al reported a PQQ-aldose dehydrogenase modified carbon paste (CP) electrode for flow-injection analysis of aldose sugars.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of a Thermostable Quinoprotein Aldose Sugar Dehydrogenase Immobilized Electrode

et al. 2013

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We fabricated a thermostable quinoprotein aldose sugar dehydrogenase (tPQQ-ASD derived from a hyperthermophilic archaeon Pyrobaculum aerophilum) immobilized electrode. The electrode was prepared by immobilizing agarose gel mixed with the enzyme and carbon nanofiber (CNF) on a carbon paste (CP) electrode containing p-benzoquinone (BQ) as an electron mediator. The electrocatalytic response was clearly observed by the addition of D-glucose at the electrode. The electrode properties such as pH, temperature dependency and substrate selectivity basically followed the enzyme properties. The current response against D-glucose increased with measurement temperatures up to 70 C, and response perturbation caused by dissolved oxygen level was not observed at the electrode. As for the results of long-term stability evaluation, the current response was stable for 30 days when the electrode was stored in HEPES buffer solution (pH 7.0) at 4 C.

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Highly Selective Needle-Type Glucose Sensors Prepared by the Immobilization of Glucose Oxidase on γ-Polyglutamic Acid Film

Cited by 6 publications

References 24 publications

Fabrication of Amperometric Glucose Sensor Using Glucose Oxidase-Cellulose Nanofiber Aqueous Solution

Fabrication of Amperometric Glucose Sensor Using Glucose Oxidase-Cellulose Nanofiber Aqueous Solution

Development and validation of dot-ELISA on modified cellulose filter paper: a simplified novel approach

Fabrication and Characterization of a Thermostable Quinoprotein Aldose Sugar Dehydrogenase Immobilized Electrode

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