Highly selective organic fluorescent probe for azide ion: formation of a “molecular ring”

Abstract

An indole based naphthalene derivative is reported as a highly selective fluorescent probe for azide ion in aqueous ethanol. The probe is applied for cell imaging of the N(3)(-) ion in contaminated living cells. Both experimental and theoretical studies have been performed to figure out the plausible mechanism of fluorescence enhancement of the probe upon binding with N(3)(-).

Cell permeable fluorescent colorimetric Schiff base chemoreceptor for detecting F− in aqueous solvent

“…In view of above we have planned to furthermore investigate the ability of PMP in detection of intracellular fluoride [31,[57][58][59][60][61]. For this study two types of cells viz.…”

Click-chemistry enabled directed evolution of glycosynthases for bespoke glycans synthesis

“…One of the major advantages of using glycosyl azides as substrates for GS reactions is that the azido moiety can be selectively conjugated to alkyne-based fluorophore groups using a modified Staudinger ligation or copper-free click-chemistry under reaction conditions compatible with the in vivo environment. 40,[46][47][48] Similar approaches can be utilized for cell-free based ultrahighthroughput screening methods for CAZyme engineering as well. 49 Interestingly, the relative difference in the fluorescence intensity of the glycosylated versus non-glycosylated triazole product influences the observed fluorescence for cells enriched in the corresponding SPAAC reaction products.…”

New potentiometric transducer based on a Mn(II) [2-formylquinoline thiosemicarbazone] complex for static and hydrodynamic assessment of azides

“…Among these approaches are: redox titrimetry [5], electron paramagnetic resonance [6], gasometry [7], spectrometry [8,9], cyclic voltammetry [10], amperometry [11], polarography [12] and chromatography [13][14][15]. Most of these methods require expensive instrumentation, rather complicated techniques, and/or sample pretreatments.…”