Highly sensitive and fast Legionella spp. in situ detection based on a loop mediated isothermal amplification technique combined to an electrochemical transduction system

Olabarría, Garbiñe; Eletxigerra, Unai; Rodríguez, Isabel; Bilbao, A.; Berganza, J.; Merino, S.

doi:10.1016/j.talanta.2020.121061

Cited by 21 publications

(17 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, although quantitative methods detecting nucleic acids, such as the ISO 12869:2019 exist, these biosensors provide qualitative results. [ 52 , 53 , 54 , 55 , 56 , 57 ]. Additionally, the biggest weakness of genosensors is their inability to detect viable but not culturable (VBNC) microorganisms [ 58 ]…”

Section: Resultsmentioning

confidence: 99%

“…Thus, although quantitative methods detecting nucleic acids, such as the ISO 12869:2019 exist, these biosensors provide qualitative results. [52][53][54][55][56][57]. Additionally, the biggest weakness of genosensors is their inability to detect viable but not culturable (VBNC) microorganisms [58] Immunosensors for Legionella are generally based on sandwich immunoassays in which a first antibody immobilised on the sensor captures the target, while a secondary labelled antibody transduces the bio-recognition into a measurable signal.…”

Section: Calibration Curve For the Detection And Quantification Of Lementioning

confidence: 99%

See 1 more Smart Citation

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

et al. 2020

View full text Add to dashboard Cite

Legionella is a pathogenic bacterium, ubiquitous in freshwater environments and able to colonise man-made water systems from which it can be transmitted to humans during outbreaks. The prevention of such outbreaks requires a fast, low cost, automated and often portable detection system. In this work, we present a combination of sample concentration, immunoassay detection, and measurement by chronoamperometry. A nitrocellulose microfiltration membrane is used as support for both the water sample concentration and the Legionella immunodetection. The horseradish peroxidase enzymatic label of the antibodies permits using the redox substrate 3,3′,5,5′-Tetramethylbenzidine to generate current changes proportional to the bacterial concentration present in drinking water. Carbon screen-printed electrodes are employed in the chronoamperometric measurements. Our system reduces the detection time: from the 10 days required by the conventional culture-based methods, to 2–3 h, which could be crucial to avoid outbreaks. Additionally, the system shows a linear response (R2 value of 0.99), being able to detect a range of Legionella concentrations between 101 and 104 cfu·mL−1 with a detection limit (LoD) of 4 cfu·mL−1.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Calibration Curve For the Detection And Quantification Of Lementioning

confidence: 99%

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

et al. 2020

View full text Add to dashboard Cite

show abstract

“…More conservatively, Fisher et al [140] advise the use of qPCR for rapid Legionella screening, where a PCR-negative result suggests no Legionella presence, and a positive output should require confirmation via culture method. Hopefully, the potentialities of molecular approaches will push the development of new methods for Legionella detection and quantification in situ and the design of simple-to-use and portable solutions for industrial application [141].…”

Section: The Scientific Perpetuation Of a Water Legionella-sampling Approachmentioning

confidence: 99%

Legionella and Biofilms—Integrated Surveillance to Bridge Science and Real-Field Demands

2021

View full text Add to dashboard Cite

Legionella is responsible for the life-threatening pneumonia commonly known as Legionnaires’ disease or legionellosis. Legionellosis is known to be preventable if proper measures are put into practice. Despite the efforts to improve preventive approaches, Legionella control remains one of the most challenging issues in the water treatment industry. Legionellosis incidence is on the rise and is expected to keep increasing as global challenges become a reality. This puts great emphasis on prevention, which must be grounded in strengthened Legionella management practices. Herein, an overview of field-based studies (the system as a test rig) is provided to unravel the common roots of research and the main contributions to Legionella’s understanding. The perpetuation of a water-focused monitoring approach and the importance of protozoa and biofilms will then be discussed as bottom-line questions for reliable Legionella real-field surveillance. Finally, an integrated monitoring model is proposed to study and control Legionella in water systems by combining discrete and continuous information about water and biofilm. Although the successful implementation of such a model requires a broader discussion across the scientific community and practitioners, this might be a starting point to build more consistent Legionella management strategies that can effectively mitigate legionellosis risks by reinforcing a pro-active Legionella prevention philosophy.

show abstract

“…At the same time, it also requires expensive professional equipment and trained operators. Other methods have also been reported for the detection of L. pneumophila in water but are less common, including IMS [ 29 , 30 ], electrochemical genosensors [ 31 ] and biosensor-based detection methods [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Most Probable Number Method for Detection and Quantification of Legionella pneumophila

Niu

Zhang

2022

Pathogens

View full text Add to dashboard Cite

The detection and enumeration of Legionella pneumophila (L. pneumophila) in water is crucial for water quality management, human health and has been a research hotspot worldwide. Due to the time-consuming and complicated operation of the plate culture method, it is necessary to adopt a fast and effective method for application. The present study aimed to comprehensively evaluate the performance and applicability of the MPN method by comparing its qualitative and quantitative results with the GB/T 18204.5-2013 and ISO methods, respectively. The qualitative results showed that 372 samples (53%) were negative for both methods; 315 samples (45%) were positively determined by the MPN method, compared with 211 samples (30%) using GB/T 18204.5-2013. The difference in the detection rate between the two methods was statistically significant. In addition, the quantitative results showed that the concentration of L. pneumophila by the MPN method was greater than ISO 11731 and the difference was statistically significant. However, the two methods were different but highly correlated (r = 0.965, p < 0.001). The specificity and sensitivity of the MPN method were 89.85% and 95.73%, respectively. Overall, the results demonstrated that the MPN method has higher sensitivity, a simple operation process and good application prospects in the routine monitoring of L. pneumophila from water samples.

show abstract

Highly sensitive and fast Legionella spp. in situ detection based on a loop mediated isothermal amplification technique combined to an electrochemical transduction system

Cited by 21 publications

References 26 publications

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

Legionella and Biofilms—Integrated Surveillance to Bridge Science and Real-Field Demands

Evaluation of a Most Probable Number Method for Detection and Quantification of Legionella pneumophila

Contact Info

Product

Resources

About