Highly sensitive and label-free digital detection of whole cell E. coli with Interferometric Reflectance Imaging

Zaraee, Negin; Kanik, Fulya Ekiz; Bhuiya, Abdul; Gong, Emily; Geib, Matthew T.; Ünlü, Neşe Lortlar; Özkumur, Ayça Yalçin; Dupuis, Jérôme; Ünlü, M. Selim

doi:10.1016/j.bios.2020.112258

Cited by 38 publications

(21 citation statements)

References 48 publications

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“…Lately, a platform for the detection of Escherichia coli whole-cells was published. The label-free interferometric reflectance imaging enhancement allowed for the sensitive detection of individual pathogens captured on the surface . The extrapolated limit of detection was calculated as 2.2 CFU mL –1 , and no sample preparation was required.…”

Section: Detectionmentioning

confidence: 99%

“…The label-free interferometric reflectance imaging enhancement allowed for the sensitive detection of individual pathogens captured on the surface. 142 The extrapolated limit of detection was calculated as 2.2 CFU mL −1 , and no sample preparation was required. PET radiotracers are, due to their unstable nature, difficult to generate precisely, especially in a clinical setting.…”

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confidence: 99%

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Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

et al. 2020

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Section: Detectionmentioning

confidence: 99%

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confidence: 99%

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

et al. 2020

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“…The dynamic range of the available technology is incredibly large, both in terms of analyte molecular weight (MW) and concentration. Nowadays, novel sensors can handle analytes as tiny as small molecules (MW < 1 kDa) [4,5,7,8] and as large as whole cells [9], in the femtomolar to millimolar range (fM-mM).…”

Section: The Importance Of Anti-fouling Materials For Label-free Kine...mentioning

confidence: 99%

The Effects of Three-Dimensional Ligand Immobilization on Kinetic Measurements in Biosensors

et al. 2022

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The field of biosensing is in constant evolution, propelled by the need for sensitive, reliable platforms that provide consistent results, especially in the drug development industry, where small molecule characterization is of uttermost relevance. Kinetic characterization of small biochemicals is particularly challenging, and has required sensor developers to find solutions to compensate for the lack of sensitivity of their instruments. In this regard, surface chemistry plays a crucial role. The ligands need to be efficiently immobilized on the sensor surface, and probe distribution, maintenance of their native structure and efficient diffusion of the analyte to the surface need to be optimized. In order to enhance the signal generated by low molecular weight targets, surface plasmon resonance sensors utilize a high density of probes on the surface by employing a thick dextran matrix, resulting in a three-dimensional, multilayer distribution of molecules. Despite increasing the binding signal, this method can generate artifacts, due to the diffusion dependence of surface binding, affecting the accuracy of measured affinity constants. On the other hand, when working with planar surface chemistries, an incredibly high sensitivity is required for low molecular weight analytes, and furthermore the standard method for immobilizing single layers of molecules based on self-assembled monolayers (SAM) of epoxysilane has been demonstrated to promote protein denaturation, thus being far from ideal. Here, we will give a concise overview of the impact of tridimensional immobilization of ligands on label-free biosensors, mostly focusing on the effect of diffusion on binding affinity constants measurements. We will comment on how multilayering of probes is certainly useful in terms of increasing the sensitivity of the sensor, but can cause steric hindrance, mass transport and other diffusion effects. On the other hand, probe monolayers on epoxysilane chemistries do not undergo diffusion effect but rather other artifacts can occur due to probe distortion. Finally, a combination of tridimensional polymeric chemistry and probe monolayer is presented and reviewed, showing advantages and disadvantages over the other two approaches.

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“…Although the presented approaches are advantageous since no sample preparation is required, the authors emphasized the requirement of detection time shortening in order to meet the demands for the biosensor as point of care devices. 41 Integration of synthetic biorecognition entities in biosensor designs is required instead of natural biorecognition elements due to the disadvantages of the stability of natural bio-recognition elements. Molecularly imprinted polymers (MIPs) have been employed to achieve analyte specificity.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in the Use of Mesoporous Silica Nanoparticles for the Diagnosis of Bacterial Infections

Karaman¹,

Pamukçu²,

Karakaplan³

et al. 2021

IJN

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Public awareness of infectious diseases has increased in recent months, not only due to the current COVID-19 outbreak but also because of antimicrobial resistance (AMR) being declared a top-10 global health threat by the World Health Organization (WHO) in 2019. These global issues have spiked the realization that new and more efficient methods and approaches are urgently required to efficiently combat and overcome the failures in the diagnosis and therapy of infectious disease. This holds true not only for current diseases, but we should also have enough readiness to fight the unforeseen diseases so as to avoid future pandemics. A paradigm shift is needed, not only in infection treatment, but also diagnostic practices, to overcome the potential failures associated with early diagnosis stages, leading to unnecessary and inefficient treatments, while simultaneously promoting AMR. With the development of nanotechnology, nanomaterials fabricated as multifunctional nano-platforms for antibacterial therapeutics, diagnostics, or both (known as “theranostics”) have attracted increasing attention. In the research field of nanomedicine, mesoporous silica nanoparticles (MSN) with a tailored structure, large surface area, high loading capacity, abundant chemical versatility, and acceptable biocompatibility, have shown great potential to integrate the desired functions for diagnosis of bacterial infections. The focus of this review is to present the advances in mesoporous materials in the form of nanoparticles (NPs) or composites that can easily and flexibly accommodate dual or multifunctional capabilities of separation, identification and tracking performed during the diagnosis of infectious diseases together with the inspiring NP designs in diagnosis of bacterial infections.

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Highly sensitive and label-free digital detection of whole cell E. coli with Interferometric Reflectance Imaging

Cited by 38 publications

References 48 publications

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

Recent Advances in Microfluidic Technology for Bioanalysis and Diagnostics

The Effects of Three-Dimensional Ligand Immobilization on Kinetic Measurements in Biosensors

Recent Advances in the Use of Mesoporous Silica Nanoparticles for the Diagnosis of Bacterial Infections

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