Highly sensitive detection of 25-HydroxyvitaminD 3 by using a target-induced displacement of aptamer

Lee, Bang Hyun; Nguyen, Van Thuan; Gu, Man Bock

doi:10.1016/j.bios.2016.08.011

Cited by 59 publications

(44 citation statements)

References 18 publications

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“…After investigating the structural properties of the used VDBA14 aptamer in the previous step, the dissociation constant of the aptamer-25(OH)D complex was determined. Therefore, MST experiments were carried out, which showed a significantly different value compared to the value reported by Lee et al via isothermal titration calorimetry (ITC) (K d = 11 nM) [17]. The K d of the used aptamer was determined to be 14.99 nM with a relatively low amplitude (amplitude = 5 AU) using MST.…”

Section: Determining the K D Of The Vdba14 Aptamermentioning

confidence: 84%

“…Phosphate-buffered saline (PBS; 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 ) was used for the blocking solution. The single-stranded DNA aptamer VDBA14 (5 -AGC-AGC-ACA-GAG-GTC-ATG-GGG-GGT-GTG-ACT-TTG-GTG-TGC-CTA-TGC-GTG-CTA-CGG-AA-3 ) was selected by Lee, Nguyen, and Gu against 25(OH)D [17]. Three complementary oligonucleotides with a length of 10 bp were designed based on the predicted aptamer structure.…”

Section: Chemicals and Dna Oligonucleotidesmentioning

confidence: 99%

“…The detection of ochratoxin A and ATP using this method has been successful in the nanomolar concentration range [15,16]. The single-stranded DNA-aptamer VDBA14 was selected against 25(OH)D by Lee, Nguyen, and Gu [17]. The aptamer has already been used in a gold nanoparticle aggregation-based assay, The single-stranded DNA-aptamer VDBA14 was selected against 25(OH)D by Lee, Nguyen, and Gu [17].…”

Section: Introductionmentioning

confidence: 99%

“…The single-stranded DNA-aptamer VDBA14 was selected against 25(OH)D by Lee, Nguyen, and Gu [17]. The aptamer has already been used in a gold nanoparticle aggregation-based assay, The single-stranded DNA-aptamer VDBA14 was selected against 25(OH)D by Lee, Nguyen, and Gu [17]. The aptamer has already been used in a gold nanoparticle aggregation-based assay, resulting in a limit of detection of 1 nM [18].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of an Aptamer Directed against 25-Hydroxyvitamin D for the Development of a Competitive Aptamer-Based Assay

et al. 2019

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Detection of the small molecule 25-hydroxyvitamin D (25(OH)D) as the most relevant marker for vitamin D supply suffers from a high variability of results using the current detection methods, such as high-performance liquid chromatography (HPLC) and immunoassays. A new detection approach using a highly specific aptamer directed against 25(OH)D was established in this study based on the target-induced dissociation (TID) sensing approach. In this work, the aptamer was investigated regarding its structural properties as well as its binding affinity by using microscale thermophoresis (MST). Moreover, complementary oligonucleotides were designed based on the aptamer structure and were evaluated in MST experiments. Binding experiments of immobilized aptamers were conducted in microarray experiments. It could be shown that the aptamer exhibited the usual B-DNA structure and did not form any G-quadruplexes. The design of complementary oligonucleotides for the TID assay identified a putative 25(OH)D binding site within the aptamer. The limit of detection of the established competitive assay was determined to be 5.4 nM, which sets the stage for the development of a biosensor system.

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Section: Determining the K D Of The Vdba14 Aptamermentioning

confidence: 84%

Section: Chemicals and Dna Oligonucleotidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of an Aptamer Directed against 25-Hydroxyvitamin D for the Development of a Competitive Aptamer-Based Assay

et al. 2019

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“…This would represent an optical aptasensor. Lee et al demonstrated the ability of a TID-based AU-NP aptasensor to monitor the small molecule hydroyvitamin D3 (25(OH)D 3 ) in the micromolar range [ 33 ]. These findings could be verified and further extended by the introduction of Cy5-labelled complementary oligonucleotides [ 34 ].…”

Section: Aptasensorsmentioning

confidence: 99%

Aptasensors for Point-of-Care Detection of Small Molecules

et al. 2020

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Aptamers, a group of nucleic acids which can specifically bind to a target molecule, have drawn extensive interest over the past few decades. For analytics, aptamers represent a viable alternative to gold-standard antibodies due to their oligonucleic nature combined with advantageous properties, including higher stability in harsh environments and longer shelf-life. Indeed, over the last decade, aptamers have been used in numerous bioanalytical assays and in various point-of-care testing (POCT) platforms. The latter allows for rapid on-site testing and can be performed outside a laboratory by unskilled labor. Aptamer technology for POCT is not limited just to medical diagnostics; it can be used for a range of applications, including environmental monitoring and quality control. In this review, we critically examine the use of aptamers in POCT with an emphasis on their advantages and limitations. We also examine the recent success of aptasensor technology and how these findings pave the way for the analysis of small molecules in POCT and other health-related applications. Finally, the current major limitations of aptamers are discussed, and possible approaches for overcoming these challenges are presented.

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Development of Aptamer‐Based Non‐labeling Methods

Hao

Wang

2018

Aptamers for Analytical Applications

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Highly sensitive detection of 25-HydroxyvitaminD 3 by using a target-induced displacement of aptamer

Cited by 59 publications

References 18 publications

Characterization of an Aptamer Directed against 25-Hydroxyvitamin D for the Development of a Competitive Aptamer-Based Assay

Characterization of an Aptamer Directed against 25-Hydroxyvitamin D for the Development of a Competitive Aptamer-Based Assay

Aptasensors for Point-of-Care Detection of Small Molecules

Development of Aptamer‐Based Non‐labeling Methods

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