Highly Sensitive Detection of Bladder Cancer-Related miRNA in Urine Using Time-Gated Luminescent Biochip

Wang, Yingqian; Li, Zhihao; Lin, Qiaosong; Wei, Yong; Wang, Jie; Li, Yingxue; Yang, Ronghua; Yuan, Quan

doi:10.1021/acssensors.9b00927

Cited by 62 publications

(48 citation statements)

References 41 publications

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“…An optical sensor using photonic crystals as a substrate was developed to detect BC-related miRNA-21 in urine 132 . Briefly, the long persistent phosphors' nanoparticles (LPPNs) were added to single-stranded DNAs (cDNAs) that are fully complementary to miRNA-21, followed by the partial hybridization with back-hole-quencher-label DNAs (BHQ-DNAs).…”

Section: Upcoming Technological Advancements To Facilitate Biomarker mentioning

confidence: 99%

“…In the detection of exosomes in cell cultural medium, their experiment demonstrated the decrease in the limit of detection (LOD) nearly two orders of magnitudes compared to conventional fluorescent detections previously. Although the aptamer sequences of ASPNC can be easily altered to achieve high flexibility for different targets, the selection of accurate sequences is challenging 130 , 132 .…”

Section: Upcoming Technological Advancements To Facilitate Biomarker mentioning

confidence: 99%

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Urine biopsy technologies: Cancer and beyond

Chen¹,

Liao²,

Li³

et al. 2020

Theranostics

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Since the discovery of circulating tumor cells in 1869, technological advances in the study of biomarkers from liquid biopsy have made it possible to diagnose disease in a less invasive way. Although blood-based liquid biopsy has been used extensively for the detection of solid tumors and immune diseases, the potential of urine-based liquid biopsy has not been fully explored. Advancements in technologies for the harvesting and analysis of biomarkers are providing new opportunities for the characterization of other disease types. Liquid biopsy markers such as exfoliated bladder cancer cells, cell-free DNA (cfDNA), and exosomes have the potential to change the nature of disease management and care, as they allow a cost-effective and convenient mode of patient monitoring throughout treatment. In this review, we addressed the advancement of research in the field of disease detection for the key liquid biopsy markers such as cancer cells, cfDNA, and exosomes, with an emphasis on urine-based liquid biopsy. First, we highlighted key technologies that were widely available and used extensively for clinical urine sample analysis. Next, we presented recent technological developments in cell and genetic research, with implications for the detection of other types of diseases, besides cancer. We then concluded with some discussions on these areas, emphasizing the role of microfluidics and artificial intelligence in advancing point-of-care applications. We believe that the benefits of urine biopsy provide diagnostic development potential, which will pave opportunities for new ways to guide treatment selections and facilitate precision disease therapies.

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Section: Upcoming Technological Advancements To Facilitate Biomarker mentioning

confidence: 99%

Section: Upcoming Technological Advancements To Facilitate Biomarker mentioning

confidence: 99%

Urine biopsy technologies: Cancer and beyond

Chen¹,

Liao²,

Li³

et al. 2020

Theranostics

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“…[ 9 ] The selectivity can also be brought when PLMs are modified with some molecules that have target identification ability, such as nucleic acid. [ 13 ] Furthermore, PLMs can exhibit better biocompatibility and lower toxicity with the modification of some less noxious chemicals such as poly(ethylene glycol) (PEG). [ 14 ] By means of surface modification, the interaction between PLMs and biological system can be easily tuned, thus endowing PLMs with immensely improved ability in bioimaging and biosensing.…”

Section: Introductionmentioning

confidence: 99%

Surface Modified Persistent Luminescence Probes for Biosensing and Bioimaging: A Review

et al. 2021

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Persistent luminescence materials (PLMs) are a class of unique luminescent materials that can remain luminescent for a few milliseconds to days without constant excitation. By virtue of the super long decay time, PLMs have been extensively explored in biosensing and bioimaging applications to elimin ate autofluorescence interference and improve signal-to-noise ratio in complex samples and tissues. However, nude PLMs often suffer from the poor stability, selectivity, and biocompatibility in biological system and in vivo, which greatly impedes their applications in biomedicine and bioanalysis. Remarkably, surface modification is a viable solution that endows PLMs with span-new features and can make PLMs suitable for organisms by altering PLMs' interaction with biological system. In this review, commonly used strategies for surface modification of PLMs are briefly introduced, and the applications of surface modified PLMs in biosensing and bioimaging as well as their challenges are summarized. Qiang Luo (top left) received his B.S. degree in chemistry from Lanzhou University in 2018. He is currently a master student under the supervision of Prof. Quan Yuan at Hunan University. His research interests include the development of functional nanomaterials for biosensing and bioimaging. Wenjie Wang (top right) received his B.S. degree from North China Electric Power University in 2018. He is currently a master student under the supervision of Prof. Quan Yuan at Hunan University. His research interests include the synthesis of inorganic nanomaterials for biological application. Jie Tan (bottom left) earned her Ph.D. degree from Zhejiang University in 2016. Then she joined the team of Prof. Weihong Tan in Hunan University as a postdoctoral fellow. Currently, she is an associate professor at Hunan University. Her main research interest is the design of chemically modified nucleic acids for biosensing. Quan Yuan (bottom right) received her B.S. degree from Wuhan University and Ph.D. degree from Peking University. Afterwards, she worked as a postdoctoral researcher in the group of Prof. Weihong Tan at University of Florida. Currently, she is a professor in Hunan University. Her research interests are the construction of nucleic acid nanomaterials and related biomedical applications.

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“…Persistent luminescence nanoparticles (PLNPs), also named as long aerglow nanoparticles, can quickly absorb the excitation energy and gradually emit luminescence for a long time aer ceasing excitation sources. [1][2][3][4] The most important feature of PLNPs is the aerglow emission, which can last for minutes, hours, and even several years. [5][6][7][8][9] Owing to their long-lasting luminescence without constant illumination, PLNPs are usually exploited as lighting components for safety signage, 10 decoration, 11,12 and displays.…”

Section: Introductionmentioning

confidence: 99%