Highly Sensitive Fluorescence Detection of Avidin/Streptavidin with an Optical Interference Mirror Slide

Yasuda, Mitsuru; Akimoto, Takuo

doi:10.2116/analsci.28.947

Cited by 7 publications

(10 citation statements)

References 32 publications

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“…1. OIM slides were firstly aminated by incubation in 1% APTMS diluted with H 2 O for 1.5 h at room temperature, (20,24) washed in H 2 O for 10 min, and then dried in an oven for 10 min at 110 °C. All experiments reported in this section were performed at room temperature.…”

Section: Immobilization Of a Probe Dnamentioning

confidence: 99%

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Enhanced Fluorescent DNA Microarray Using an Optical Interference Mirror Slide

Yasuda

Akimoto

2018

Sensors and Materials

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Enhanced fluorescence using an optical interference mirror (OIM) slide consisting of a metal film and a transparent dielectric thin film is used to improve the sensitivity of a DNA microarray. Twenty-one bases of a probe DNA are immobilized on the OIM slide fabricated with Ag and Al 2 O 3 , and 21 bases of target DNA, whose sequence is complementary to that of the probe DNA, are detected. After evaluating the hybridization specificity between the probe DNA and the target DNA, the sensitivity improvement is estimated. As a result, a 50-fold improvement can be achieved compared with a bare glass slide.

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Section: Immobilization Of a Probe Dnamentioning

confidence: 99%

“…The sensitivity improvement for Cy5-labeled streptavidin detection using the OIM slide was reported to be 71-fold (20) compared with that using the bare glass slide. Therefore, a 50-fold sensitivity improvement for DNA detection can be considered reasonable.…”

Section: Detection Of Target Dnamentioning

confidence: 99%

Enhanced Fluorescent DNA Microarray Using an Optical Interference Mirror Slide

Yasuda

Akimoto

2018

Sensors and Materials

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“…9 Such an enhancement is primarily due to double interference of the excitation light and the fluorescence in the dielectric layer. 13 From the viewpoint of providing a high magnitude of enhancement, the OIM slide has been applied to biomolecular analysis, 14 environment monitoring, 15 and cell imaging. 16,17 We have previously succeeded in enhancing the fluorescence of rhodamine B by as much as 200-fold through the use of an OIM slide with an approximately 90-nm-thick film of Al2O3 as the dielectric material.…”

Section: Introductionmentioning

confidence: 99%

High-Contrast Fluorescence Imaging Based on the Polarization Dependence of the Fluorescence Enhancement Using an Optical Interference Mirror Slide

Yasuda

Akimoto

2015

ANAL. SCI.

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High-contrast fluorescence imaging using an optical interference mirror (OIM) slide that enhances the fluorescence from a fluorophore located on top of the OIM surface is reported. To enhance the fluorescence and reduce the background light of the OIM, transverse-electric-polarized excitation light was used as incident light, and the transverse-magnetic-polarized fluorescence signal was detected. As a result, an approximate 100-fold improvement in the signal-to-noise ratio was achieved through a 13-fold enhancement of the fluorescence signal and an 8-fold reduction of the background light.

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“…(11,12) The enhancement effect is explained by the double interference of both the excitation light and fluorescence in the optical interference layer, which may account for the fact that the fluorescence enhancement is periodically dependent on the thickness of the optical interference layer. Other multilayered substrates have been also reported employing Al 2 O 3 (13)(14)(15)(16)(17) and SiO 2 (9,18) as the materials of the optical interference layers instead of LiF; these have been applied to affinity assays, (9,17) mutagen detection, (16) and cell imaging. (13) In this study, we propose plasma-polymerized films (PPFs) as new alternatives to Al 2 O 3 or SiO 2 as optical interference materials for multilayered substrates.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Enhancement of Immunoassay Using Multilayered Glass Substrates Modified with Plasma-Polymerized Films

2015

Sensors and Materials

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The plasma polymerization technique, which enables deposition of thin and pinhole free films, was employed to fabricate multilayered substrates for fluorescencebased sensitive immunoassay. A glass slide was modified with a 200-nm-thick Ag layer as a metal mirror and further coated with plasma-polymerized films (PPFs) up to 120 nm thick as the optical interference layers. Cy3-labeled antibody was spotted on the resulting multilayered substrate and the fluorescence image was observed. The Cy3 fluorescence was highly enhanced on the multilayered substrate compared with that observed on the area without PPFs. The maximum fluorescence intensity was obtained when PPF thickness was 63 nm, showing a 30-fold enhancement of fluorescence compared with that obtained from the unmodified substrate. The multilayered substrates with PPF on which multiple proteins were immobilized were further utilized to perform immunoassays, resulting in specific and highly enhanced fluorescence detection.

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Highly Sensitive Fluorescence Detection of Avidin/Streptavidin with an Optical Interference Mirror Slide

Cited by 7 publications

References 32 publications

Enhanced Fluorescent DNA Microarray Using an Optical Interference Mirror Slide

Enhanced Fluorescent DNA Microarray Using an Optical Interference Mirror Slide

High-Contrast Fluorescence Imaging Based on the Polarization Dependence of the Fluorescence Enhancement Using an Optical Interference Mirror Slide

Fluorescence Enhancement of Immunoassay Using Multilayered Glass Substrates Modified with Plasma-Polymerized Films

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