Highly sensitive fluorescent detection of EDIL3 overexpressed exosomes for the diagnosis of triple-negative breast cancer

Abstract

EDIL3 is a strong and highly accurate diagnostic marker for breast cancer, meanwhile, EDIL3 overexpressed exosomes are novel biomarkers for the early diagnosis of triple-negative breast cancer (TNBC). Here, we proposed a fluorescent detection method for EDIL3 overexpressed exosomes, which is simple and sensitive. Basically, we utilized a magnetic nanospheres (MNS) based liquid sandwich immunoassay strategy. MNS were modified with CD63 aptamers, which can immunologically bound to the CD63 protein on the surface of exosomes. Alexa Fluor 647 labeled anti-EDIL3 antibodies (Anti-EDIL3/AF647) were used as the fluorescent probes to recognize the EDIL3 on exosomes derived from a TNBC cell line (MDA-MB-231). With the target TNBC exosomes present, sandwich structures containing MNS, exosomes and fluorescent probes were formed. After magnetic purification, optical super resolution imaging of the products was conducted to check the specificity of the assay. In addition, fluorescence signals of the products were detected to quantitatively analyze the EDIL3 overexpressed exosomes. The linear range was found to be 7.78 × 10¹ to 7.78× 10⁶ particles/μL. The detection limit was approximately 10 particles/μL. The feasibility of the method for the detection of exosomes in complex biological samples was also demonstrated. Such a simple and sensitive detection method for EDIL3 overexpressed exosomes holds a great potential in clinical diagnosis of TNBC.

System analysis based on Anoikis-related genes identifies MAPK1 as a novel therapy target for osteosarcoma with neoadjuvant chemotherapy

“…EDIL3 was identified as a novel regulator of epithelial-mesenchymal transition (EMT), contributing to angiogenesis, metastasis, and recurrence of hepatocellular carcinoma [47]. EDIL3 is also a strong and highly accurate diagnostic marker for breast cancer [48]. Moreover, in accordance with previous studies, we found that tumor-derived EDIL3 was involved in tumor-associated bone loss [49].…”

The Advances and Applications of Characterization Technique for Exosomes: From Dynamic Light Scattering to Super-Resolution Imaging Technology

“…The study found that the frequency and intensity of HLA-DR expression in the MMD-secreted EV derived from the lungs of cancer patients was higher, suggesting the enrichment of macrophages in the lungs of cancer patients [90]. In the same year, Wei et al proposed a fluorescence detection method for EDIL3-overexpressed exosomes through SMLM and proposed that EDIL3-overexpressed exosomes are novel biomarkers for the early diagnosis of triple-negative breast cancer (TNBC), which have great potential in the clinical diagnosis of TNBC [100]. The DNA-PAINT technology focused on reducing the resolution to the molecular level early.…”

Oncogenic and immunological role of EDIL3 in human tumours: From pan-cancer analysis to validation in gastric cancer

“…Structurally, EDIL3 comprises two discoidin I-like repeats and three EGF-like repeats that interact with integrin to modulate endothelial cell function [ 6 , 7 ]. Recent studies have reported that EDIL3 plays critical roles in tumourigenesis, metastasis, and angiogenesis [ [8] , [9] , [10] , [11] , [12] ]. For instance, EDIL3 is overexpressed in hepatocellular carcinoma and promotes angiogenesis, invasion, and migration in vitro [ 13 ].…”