Highly sensitive optical chip immunoassays in human serum

Schneider, Bernard H.; Dickinson, E.L; Vach, M.D; Hoijer, J.V; Howard, L.V

doi:10.1016/s0956-5663(00)00056-7

Cited by 105 publications

(73 citation statements)

References 35 publications

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“…Use of heterogeneous protein mixture and detection of reacting antibody to components of the heterogeneous protein mixture is a marked advance in protein chip technology. Although human serum was used in pre-clinical tests (Schneider, 2000), human serum was used for the clinical diagnostic purpose for the analysis of allergen-specific IgE levels in this study. Spotting of heterogeneous protein for protein chip and use of serum directly for tests might be the most important factors for the clinical application of protein chip in other clinical fields.…”

Section: Discussionmentioning

confidence: 99%

Quantitative measurement of serum allergen-specific IgE on protein chip

Kim

Park²,

Cho³

et al. 2002

Exp Mol Med

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Type I allergy is an immunoglobulin E (IgE)-mediated hypersensitivity disease inflicting more than quarter of the world population. In order to identify allergen sources, skin provocation test and IgE serology was performed using allergen extracts. Such process identifies allergen-containing sources but cannot identify the disease-eliciting allergenic molecules. Recently, microarray technology has been developed for allergen-specific IgE detection using rolling circle amplification. This study was carried out to evaluate protein chip technology for the quantitative measurement and limits of sensitivity of multiple allergen-specific IgE by an immunofluorescence assay. Significance of positive calibrators was tested using purified human IgE. Dermatophagoides pteronyssinus (Dp), egg white, milk, soybean, and wheat were used as allergens and human serum albumin as negative control. Sensitivity and clinical efficacy of protein chip were evaluated using allergy immune serum for Dp. The fluorescent intensities for purified human IgE as calibrator were well correlated with the concentrations of human IgE. Twofold dilution of serum allowed an optimal reaction with Dp (1 mg/ml) at which serum Dp-specific IgE levels by protein chip were compatible with those by UniCap®. The sensitivity of protein chip in this study was found at level of 1 IU/ml of IgE. Dp-specific IgE levels by protein chip correlated well with those of UniCap® by comparing 10 atopic dermatitis. Additional 18 sera were tested for above multiple antigens other than Dp and significant results were obtained for many antigens as well as Dp. These results indicated that spotting of heterogeneous protein mixture on protein chip and the quantitative measurement of serum allergen-specific IgE levels using immunofluorescence assay can be successfully applied in the clinical laboratory for the diagnosis of allergy and could be applied to diagnosis of autoimmune and infectious diseases

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Section: Discussionmentioning

confidence: 99%

Quantitative measurement of serum allergen-specific IgE on protein chip

Kim

Park²,

Cho³

et al. 2002

Exp Mol Med

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“…116) Furthermore, functionalized nanoparticles have been used as optical sensors to quantitatively assay biomolecules such as human chorionic gonadotropin (hCG) in human serum. 117) Darkow et al demonstrated removal of endotoxins from water, dialysis fluid, plasma or blood by using functionalized nanoparticles. 118) Another approach is the solid nanoshell polymer composite, which is a photo-thermally triggered drug delivery system.…”

Section: Copolymerized Peptide Nanoparticles (Cpp)mentioning

confidence: 99%

Nanocarriers: Promising Vehicle for Bioactive Drugs

Rawat

Singh

Saraf

2006

Biological & Pharmaceutical Bulletin

395

183

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Development of new delivery systems that deliver the potential drug specifically to the target site in order to meet the therapeutic needs of the patients at the required time and level remains the key challenge in the field of pharmaceutical biotechnology. Developments in this context to achieve desired goal has led to the evolution of the multidisciplinary field nanobiotechnology which involves the combination of two most promising technologies of 21st century-biotechnology and nanotechnology. Nanobiotechnology encompasses a wide array of different techniques to improve the delivery of biotech drugs, and nanoparticles offer the most suitable form whose properties can be tailored by chemical methods. This review highlights the different types of nanoparticulate delivery systems employed for biotech drugs in the field of molecular medicine with a short overlook at its applications and the probable associated drawbacks.

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“…Optical interferometers have already used in detection of surface bound bio-reactants such as bacteria, spores, toxins, viruses, and proteins (Schneider et al, 2000;Schmitt et al, 2007). These devices are based on evanescent field sensing.…”

Section: Interferometer-based Biosensorsmentioning

confidence: 99%

Nanodiagnostics: a new frontier for veterinary and medical sciences

Lambe¹,

Minakshi²,

Brar³

et al. 2016

JEBAS

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Infectious diseases are one of the greatest threats to animal and human population living in the developing world. These diseases have capacity to instigate in a small area and then open out very fast to the rest of the world and causing a heavy pandemic situation, for example; avian influenza pandemic. Such diseases infect large masses of population and may lead to loss of lives and also incur huge economic losses. Therefore, the best way to control these diseases is by diagnosing it at a very primary level and taking necessary precautionary measures so as to avoid the spread. Since last few years, the diagnostic approach has changed from tedious molecular biological techniques, to easy and rapid diagnostic techniques. Nanotechnology has extended the molecular diagnostics limit to nanoscale. These developed techniques do not require sophisticated laboratories and expert personnel, and hence are a cheap diagnostic approach. These assays can also be performed at the field level where the patient is present and get the results there itself. Hence, they are also called as pen side test or lab on chip diagnostic assays. The biological tests using nanotechnology become quicker, more flexible and more sensitive. These techniques have greatly influenced the diagnostic approach in the veterinary as well as medical field. Especially in the developing countries such as India, where the laboratory services are not

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Highly sensitive optical chip immunoassays in human serum

Cited by 105 publications

References 35 publications

Quantitative measurement of serum allergen-specific IgE on protein chip

Quantitative measurement of serum allergen-specific IgE on protein chip

Nanocarriers: Promising Vehicle for Bioactive Drugs

Nanodiagnostics: a new frontier for veterinary and medical sciences

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