2010
DOI: 10.1117/1.3302810
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Highly sensitive optical detection of specific protein in breast cancer cells using microstructured fiber in extremely low sample volume

Abstract: Abstract.A simple optical method using hollow-core photonic crystal fiber for protein detection has been described. In this study, estrogen receptor ͑ER͒ from a MCF-7 breast carcinoma cell lysates immobilized inside a hollow-core photonic crystal fiber was detected using anti-ER primary antibody with either Alexa ™ Fluor 488 ͑green fluorescent dye͒ or 555 ͑red Fluorescent dye͒ labeled Goat anti-rabbit IgG as the secondary antibody. The fluorescence fingerprints of the ER␣ protein were observed under fluorescen… Show more

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Cited by 48 publications
(16 citation statements)
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“…Conventional fiber probe imaging methods use widefield and confocal approaches2431. In this work, we have implemented targeted imaging approaches (In other words to image the regions of interest in FOV).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Conventional fiber probe imaging methods use widefield and confocal approaches2431. In this work, we have implemented targeted imaging approaches (In other words to image the regions of interest in FOV).…”
Section: Resultsmentioning
confidence: 99%
“…However, high resolution fiber probes with targeted shape selective optical switching are not explored to their potential192324252627. In this article, we have developed a novel, high-speed probe that can selectively (targeted) illuminate and manipulate any region in the sample space with microscale resolution.…”
mentioning
confidence: 99%
“…Currently, various bio-markers, such as epidermal growth factor receptor (EGFR), human epidermal growth factor receptor 2 (HER2), prostate-specific antigen (PSA), carcinoembryonic antigen (CEA), tumor necrosis factor-α (TNF-α), and calreticulin (CRT), are under clinical study for diagnosis of cancer. 189191 Detecting these biomarkers at an early stage of malignancy can contribute to better treatment outcomes and significantly increase the quality of life for cancer patients.…”
Section: Multi-target Detection Capability For Pc-based Biosensorsmentioning
confidence: 99%
“…Unlike conventional fibers, photonic crystal fibers are made of pure silica glass (SiO 2 ) without any doping. Hence it is biocompatible and chemically inert [12]. Further, the capillary tubes present in the PCFs have a good surfaceto-volume ratio.…”
Section: Introductionmentioning
confidence: 99%