Highly specific detection of KRAS single nucleotide polymorphism by asymmetric PCR/SERS assay

Lyu, Nana; Rajendran, Vinoth Kumar; Li, Jun; Engel, Alexander; Molloy, Mark P.; Wang, Yuling

doi:10.1039/d1an01108a

Cited by 13 publications

(13 citation statements)

References 39 publications

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“…AuNPs have also been used for the detection of single-nucleotide polymorphisms [119]. Analyses are primarily conducted by hybridizing a DNA microarray with single-strand DNA-AuNPs, and interpretation is obtained through analysis of various signals, including resonance light scattering [120] and SERS [121].…”

Section: Single-point Mutations or Single-nucleotide Polymorphism (Snp)mentioning

confidence: 99%

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Chan

Chang

et al. 2023

Molecules

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Molecular biology applications based on gold nanotechnology have revolutionary impacts, especially in diagnosing and treating molecular and cellular levels. The combination of plasmonic resonance, biochemistry, and optoelectronic engineering has increased the detection of molecules and the possibility of atoms. These advantages have brought medical research to the cellular level for application potential. Many research groups are working towards this. The superior analytical properties of gold nanoparticles can not only be used as an effective drug screening instrument for gene sequencing in new drug development but also as an essential tool for detecting physiological functions, such as blood glucose, antigen-antibody analysis, etc. The review introduces the principles of biomedical sensing systems, the principles of nanomaterial analysis applied to biomedicine at home and abroad, and the chemical surface modification of various gold nanoparticles.

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Section: Single-point Mutations or Single-nucleotide Polymorphism (Snp)mentioning

confidence: 99%

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Chan

Chang

et al. 2023

Molecules

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“…Accordingly, this SERS strategy has revealed tremendous clinical application prospect because KRAS G12 V is closely related to many cancers with high incidence rate. [107] The nicking endonuclease signal amplification (NESA) is a common approach for distinguishing specific DNA sequences. Liu et al proposed a SERS sensing strategy based on NESA for successfully detecting oral cancer related gene ORAOV1.…”

Section: Nucleic Acidmentioning

confidence: 99%

“…Exosomes, as extracellular vesicles with the diameter of 30-150 nm secreted by cells, are widely distributed in human body fluids and carry massive nucleic acid, protein, lipid, and other [107] with permission from RSC. (B) The SERS sensing strategy based on NESA for oral cancer DNA analysis.…”

Section: Exosome and Circulating Tumor Cellmentioning

confidence: 99%

Recent Research Progress of Surface‐Enhanced Raman Scattering Dominated Analysis Strategies in Early Diagnosis of Diseases

Chen

Zheng

Tang

et al. 2023

Chemistry An Asian Journal

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Timely and powerful diagnostic means can achieve better therapeutic effects, reduce disease torment, and improve survival rate. As a powerful non‐invasive spectroscopy technology, surface‐enhanced Raman scattering (SERS) have testified to be a great potential candidate for extensive early clinical disease diagnosis. In recent years, the introduction of SERS label, combined with other analysis modes or artificial intelligence, and the emergence of miniaturized devices have made SERS technology more advantageous in early diagnosis of diseases. This review focuses on the research progress of SERS dominated analytical strategies in the field of early disease diagnosis in the past five years. The main content includes the application of label‐free SERS detection; the construction of label SERS methodologies for various disease markers; SERS dominated multimode early disease diagnosis strategies; integration of SERS and artificial intelligence; portable Raman equipment and SERS imaging; and opportunities and trends for SERS diagnostic technology in the future.

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“…The development of a SERS-based strategy for identifying the DNA of eukaryotes like plants and animals could pave the way for early disease detection in the world of diagnostics. SERS has already been shown to be an effective technique for identifying significant mutations, 35 particularly mutations linked to colorectal cancer 36 in human cell lines. Strategies for animals and humans must go through extensive trial and testing, as well as numerous ethical clearances before they can be converted into diagnostic tools.…”

Section: Introductionmentioning

confidence: 99%

SERS combined with PCR as a potent tool for detecting mutations: a case study of tomato plants

Sen

Chalapathi

Targolli³

et al. 2022

RSC Adv.

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Highly specific detection of KRAS single nucleotide polymorphism by asymmetric PCR/SERS assay

Abstract: The molecular diagnosis of KRAS mutations has become crucial for clinical decision-making in colorectal cancer (CRC) treatments. Currently, the common methods for detecting mutations are based on quantitative PCR, DNA...

Cited by 13 publications

References 39 publications

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Recent Research Progress of Surface‐Enhanced Raman Scattering Dominated Analysis Strategies in Early Diagnosis of Diseases

SERS combined with PCR as a potent tool for detecting mutations: a case study of tomato plants

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