Highly specific label-free protein detection from lysed cells using internally referenced microcantilever sensors

Shu, Wenmiao; Laurenson, Sophie; Knowles, Tuomas P. J.; Ferrigno, Paul Ko; Seshia, Ashwin A.

doi:10.1016/j.bios.2008.03.036

Cited by 48 publications

(33 citation statements)

References 24 publications

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“…Their specificity appeared unaltered between the scaffolds, as none of the peptide inserts recognise CDK4 in yeast-two-hybrid assays when presented in the context of TrxA or of STM. The interactions of some of these peptide aptamers with the cyclin-dependent kinases CDK2 or CDK4 in vitro have been previously studied by means of electrochemical impedance spectroscopy using microelectrodes [17], dual polarization interferometry [11] and microcantilevers [18]. In vitro, STM-pep2 is reported to interact with CDK2 and not with CDK4, while STM-pep9 interacts with both CDK2 and CDK4.…”

Section: Resultsmentioning

confidence: 97%

Label-Free Detection of Protein interactions with peptide aptamers by open circuit potential measurement

Estrela

Paul

Keighley

et al. 2008

Electrochimica Acta

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Section: Resultsmentioning

confidence: 97%

Label-Free Detection of Protein interactions with peptide aptamers by open circuit potential measurement

Estrela

Paul

Keighley

et al. 2008

Electrochimica Acta

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“…In contract, the uses of artificial protein binding ligands based on DNA [53] and peptide [54] aptamers were also reported. Specific detection of a protein biomarker (i.e.…”

Section: Biosensing Applicationsmentioning

confidence: 98%

“…CDK2 at 80 ng/mL) was achieved in a complex biological environment (e.g. cell lysate) [54]. In addition to protein binding, the aggregation of protein molecules on surface to form insoluble protein fibrils was first studied using microcantilever sensors by Knowles et al [55].…”

Section: Biosensing Applicationsmentioning

confidence: 99%

Microcantilever Biosensors: Probing Biomolecular Interactions at the Nanoscale

Li¹,

Shu

2011

COC

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Microcantilever based biosensors have attracted much attention as a label-free, real-time and highly sensitive approach to the detection of biomolecules. When specific biomolecular interactions occur between a receptor immobilized on one side of a cantilever and a target analyte in solution, a mechanical bending of the cantilever results due to a change in surface stress, thereby translating biochemical interactions into a concentration-dependent nanomechanical response of the microcantilevers. In this review, we present a general overview of the operation principles, fabrication and preparation of microcantilever biosensors and discuss their uses for a wide range of biosensing applications. The focus of the review is given to probing the biomolecular interactions at the solid-liquid interface by measuring the cantilever bending. In addition to the label-free detection of DNA, protein and cells, the emphasis is also made to discuss about the new development of microcantilever biosensors for label-free probing nanoscale conformational changes of DNA, protein and polymers, and real-time detection of nanomechanical forces from living cells. Finally, the outlook for future development work and challenges is also discussed.

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“…Each nanowire acts as a field-effect transistor that produces a measurable change in conductance when a binding event takes place on the wire surface, and an array of nanowires can be constructed on a single chip for multiplex detection [128]. In a nanocantilever system, a series of 100-750 µm long × 20-100 µm wide × 0.6-1.0 µm thick functionalized cantilevers act as sensors when changes in mechanical bending and vibrational frequency are identified in the event of binding [129], and even single-base mismatches in DNA are discernable [130,131]. In 2009, an effort was initiated in London, UK, to develop a nanocantilever device for the bedside detection and monitoring of HIV/AIDS [204].…”

Section: Future Perspectivementioning

confidence: 99%

HIV Diagnostics: Challenges and Opportunities

Wong

Hewlett

2010

HIV Ther.

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Accurate HIV diagnostic testing continues to pose challenges, but there are also opportunities for assay performance improvements in key areas for specific intended-use settings. The genetic diversity of HIV can result in false and discordant results in assays that fail to detect new variant strains. The use of antiretroviral therapies has resulted in drug-resistant variants that require monitoring by sequencing and genotyping methods. Nucleic acid testing is the most sensitive and reliable platform for detection, but it is costly and limited to centralized testing facilities, making implementation difficult in resource-limited settings where HIV has hit the hardest. Rapid antibody tests suitable for point-of-care use are becoming more accessible in resource-limited settings, but these tests may not detect HIV during the acute infection stage. Emerging antigen/antibody combination assays are viable alternatives to nucleic acid testing for diagnosis of recent infections. Although patient monitoring (e.g., via CD4+ T-cell count and viral load determination) and infant diagnoses still rely on clinical laboratory-based testing, point-of-care options are being developed. There are other technical challenges to HIV diagnostic testing and emerging biodetection technologies that may be able to address them, but they are not yet proven.

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Highly specific label-free protein detection from lysed cells using internally referenced microcantilever sensors

Cited by 48 publications

References 24 publications

Label-Free Detection of Protein interactions with peptide aptamers by open circuit potential measurement

Label-Free Detection of Protein interactions with peptide aptamers by open circuit potential measurement

Microcantilever Biosensors: Probing Biomolecular Interactions at the Nanoscale

HIV Diagnostics: Challenges and Opportunities

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