Highly stable bilayer lipid membranes (BLMs) formed on microfabricated polyimide apertures

Eray, M.; Dogan, N.S.; Liu, Liangjun; Koch, Alan; Moffett, David F.; Silber, M.; Wie, Bernard J. Van

doi:10.1016/0956-5663(94)80034-0

Cited by 35 publications

(31 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The tapered aperture edge reduces stress on the bilayer at the point of contact with the edge, leading to increased BLM stability. A similar enhancement in BLM stability based on tapered apertures was also reported by Eray et al (13) and Kalsi et al (14). The key feature of this approach is that one can form mechanically stable BLMs while maintaining a large membrane area of several tens of micrometers.…”

Section: Introductionsupporting

confidence: 85%

“…The use of a nonvolatile hydrocarbon solvent is a common approach not only to improve BLM stability but also to promote channel integration (7)(8)(9)(10)(11)(12)(13)(14)(18)(19)(20)(21)(22)(24)(25)(26)(27)(28)(29). Several researchers reported that the presence of nonvolatile organic solvent in BLMs appeared to promote channel reconstitution (28,29).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Reconstitution of Human Ion Channels into Solvent-free Lipid Bilayers Enhanced by Centrifugal Forces

Hirano‐Iwata

Ishinari

Yoshida

et al. 2016

Biophysical Journal

View full text Add to dashboard Cite

Artificially formed bilayer lipid membranes (BLMs) provide well-defined systems for functional analyses of various membrane proteins, including ion channels. However, difficulties associated with the integration of membrane proteins into BLMs limit the experimental efficiency and usefulness of such BLM reconstitution systems. Here, we report on the use of centrifugation to more efficiently reconstitute human ion channels in solvent-free BLMs. The method improves the probability of membrane fusion. Membrane vesicles containing the human ether-a-go-go-related gene (hERG) channel, the human cardiac sodium channel (Na v 1.5), and the human GABA A receptor (GABA A R) channel were formed, and the functional reconstitution of the channels into BLMs via vesicle fusion was investigated. Ion channel currents were recorded in 67% of the BLMs that were centrifuged with membrane vesicles under appropriate centrifugal conditions (14-55 Â g). The characteristic channel properties were retained for hERG, Na v 1.5, and GABA A R channels after centrifugal incorporation into the BLMs. A comparison of the centrifugal force with reported values for the fusion force revealed that a centrifugal enhancement in vesicle fusion was attained, not by accelerating the fusion process but by accelerating the delivery of membrane vesicles to the surface of the BLMs, which led to an increase in the number of membrane vesicles that were available for fusion. Our method for enhancing the probability of vesicle fusion promises to dramatically increase the experimental efficiency of BLM reconstitution systems, leading to the realization of a BLM-based, high-throughput platform for functional assays of various membrane proteins.

show abstract

Section: Introductionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Reconstitution of Human Ion Channels into Solvent-free Lipid Bilayers Enhanced by Centrifugal Forces

Hirano‐Iwata

Ishinari

Yoshida

et al. 2016

Biophysical Journal

View full text Add to dashboard Cite

show abstract

“…suggests that the annulus -septum contact angle should be small for a stable arrangement of BLMs (19,25,26), the tapered aperture edge, as well as nanometre thickness of the septum, is favourable to improve the BLM stability. Formation of free-standing BLMs across the aperture was investigated by the folding method.…”

Section: Supramolecular Chemistry 409mentioning

confidence: 99%

Stable lipid bilayers based on micro- and nano-fabrication

Hirano‐Iwata

Oshima

Nasu

et al. 2010

Supramolecular Chemistry

View full text Add to dashboard Cite

In this review, we will discuss our recent approaches for improving the mechanical stability of free-standing bilayer lipid membranes (BLMs) by combining with BLM formation and micro-and nano-fabrication techniques. BLMs were prepared across a microaperture fabricated in silicon (Si) chips or nanoporous alumina films, and their mechanical stability and electric properties were investigated. BLMs spanned over the porous alumina showed background noise currents small enough for recording activities of low-conductance channels, though further stability enhancement of porous alumina films was necessary. BLMs suspended in a thin Si 3 N 4 septum showed a dramatic improvement of BLM stability. The BLMs were resistant to a voltage of^1 V and the membrane lifetime was 15 -43 h with and without incorporated channels. The membrane containing gramicidin channel exhibited tolerance to repetitive solution exchanges, though the electric properties of the BLMs are necessary to be improved. The realisation of BLMs having both mechanical stability and proper electric properties will open up a variety of applications including highly sensitive biosensors and high-throughput drug screenings for ion channels.

show abstract

“…A lipid bilayer is formed by either the painting method (Miller, 1986;Fujiwara et al, 2003) or Langmuir-Blodgette method (Montal and Mueller, 1972). Recently, microfabrication technology has been attracting attention to construct lipid bilayers in precisely defined small apertures (Schmidt et al, 2000;Pantoja et al, 2001;Peterman et al, 2002;Fertig et al, 2002;Eray et al, 1994;Cheng et al, 2001;Mayer et al, 2003;Ide and Ichikawa, 2005). Yet, the bilayer reconstitution methods in these studies are based on conventional methods.…”

Section: Introductionmentioning

confidence: 99%

Electrophysiological recordings of single ion channels in planar lipid bilayers using a polymethyl methacrylate microfluidic chip

Suzuki

Tabata

Noji

et al. 2007

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Highly stable bilayer lipid membranes (BLMs) formed on microfabricated polyimide apertures

Cited by 35 publications

References 13 publications

Reconstitution of Human Ion Channels into Solvent-free Lipid Bilayers Enhanced by Centrifugal Forces

Reconstitution of Human Ion Channels into Solvent-free Lipid Bilayers Enhanced by Centrifugal Forces

Stable lipid bilayers based on micro- and nano-fabrication

Electrophysiological recordings of single ion channels in planar lipid bilayers using a polymethyl methacrylate microfluidic chip

Contact Info

Product

Resources

About