Highly Stable Mutant Bacterial Formate Dehydrogenase with Improved Catalytic Properties

Pometun, A. A.; Shirokova, A. A.; Galanicheva, N. P.; Shaposhnikov, Leonid A.; Atroshenko, D. L.; Pometun, E. V.; Тишков, В. И.; Савин, С. С.

doi:10.3103/s0027131423010042

Cited by 3 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PseFDH wt [ Дальнейшее улучшение параметров было получено после второй замены C145A [33]. В результате многоточечный мутант PseFDH SM4A M3 с этими двумя заменами (остальные мутации повышали термостабильность, см.…”

Section: инженерия основных свойств формиатдегидрогеназunclassified

“…ниже) имеет величину k кат. , равную 7,6 с -1 , а значения К М по NAD + и формиату составляют 22 мкМ и 1,2 мМ соответственно [33].…”

Section: инженерия основных свойств формиатдегидрогеназunclassified

“…На сегодняшний момент вариант мутантной PseFDH SM4A М3 является наиболее эффективным биокатализатором среди описанных в литературе формиатдегидрогеназ [33]. Тем не менее, увеличения с помощью белковой инженерии удельной активности известных FDH выше 10-11 ед./мг белка достичь не удалось.…”

Section: инженерия основных свойств формиатдегидрогеназunclassified

“…Это формиатдегидрогеназа из бактерий Rhodococcus jostii (RjoFDH) [18] [61] и PseFDH [30], не влияя на каталитические параметры и термостабильность этих ферментов. Позднее в PseFDH была сделана замена C145A, которая не влияла на активность, но уменьшала К М фермента по формиату в два раза и приводила к дополнительному снижению термостабильности [33]. Для восстановления термостабильности мутантной PseFDH C145A/C255A в фермент были введены дополнительные замены.…”

Section: инженерия основных свойств формиатдегидрогеназunclassified

“…Для восстановления термостабильности мутантной PseFDH C145A/C255A в фермент были введены дополнительные замены. На данный момент можно констатировать, что финальный вариант мутанта PseFDH SM4A M3 [33] является оптимальным для применения на практике.…”

Section: инженерия основных свойств формиатдегидрогеназunclassified

See 4 more Smart Citations

Formate Dehydrogenase: From Nad(p)h Regeneration To the Target in Pathogens Biofilms, a Component Of Highly Efficient Hybrid Biocatalysts and Co2 Fixation From the Atmosphere

Tishkov,

Pometun,

Savin

2023

Lomonosov Chemistry Journal

View full text Add to dashboard Cite

NAD(P)+ -dependent formate dehydrogenase (EC 1.2.1.2, FDH) catalyzes the simple from chemical and biological point of view reaction of formate ion oxidation to carbon dioxide with corresponding reduction of NAD(P)+ to NAD(P) H. Advances in the life sciences have shown that this reaction plays an extremely important role in a wide variety of organisms. The areas and types of practical applications of FDH are also permanently expanding. In this review we considered the main stages in the development of understanding and knowledge about the role of formate dehydrogenase in living systems. Achievements in creation of highly effi cient catalysts based on FDH for classic biotechnology as well as for new areas are also considered. The importance of appropriate choice of the initial FDH for the creation of a biocatalyst with the required and prescribed properties with minimal costs is shown. The prospects for the use of FDH for the fixation of CO2 are discussed.

show abstract

Section: инженерия основных свойств формиатдегидрогеназunclassified

See 3 more Smart Citations

Formate Dehydrogenase: From Nad(p)h Regeneration To the Target in Pathogens Biofilms, a Component Of Highly Efficient Hybrid Biocatalysts and Co2 Fixation From the Atmosphere

Tishkov,

Pometun,

Savin

2023

Lomonosov Chemistry Journal

View full text Add to dashboard Cite

show abstract

Formate Dehydrogenase: From NAD(P)H Regeneration to Targeting Pathogen Biofilms, Composing Highly Efficient Hybrid Biocatalysts and Atmospheric CO2 Fixation

Tishkov,

Pometun,

Savin

2023

Moscow Univ. Chem. Bull.

View full text Add to dashboard Cite

Biocatalysis: modern problems and applications

Varfolomeev,

Švedas,

Efremenko

et al. 2024

RUSS CHEM REV

View full text Add to dashboard Cite

The review is devoted to analysis of recent advances, problems, and practical applications of enzymes in the framework of chemical enzymology and enzyme engineering. The achievements in the fundamental understanding of the molecular mechanisms of the catalytic cycle of enzymatic reactions are considered using quantum/molecular mechanics methods with supercomputer technologies and bioinformatics methods. The construction of protein biocatalysts with new properties is a fundamentally significant methodology of the bioengineering approach to solving practical problems, which is demonstrated by a number of examples. The increasing role of biocatalysis in medicine and biomedical research is illustrated by solving the problems of antibiotic synthesis and overcoming bacterial resistance to them, studying the mechanisms of neurodegenerative diseases and developing drugs to treat Alzheimer's disease, and investigation of biocatalytic processes of DNA repair and the role of mechanisms of functioning of heme peroxidases in the human body. The use of enzymes that destroy various endogenous and exogenous toxicants has been greatly developed in recent decades. The problems of using enzymes in therapy and drug delivery are analyzed. The fundamental role of enzymes in modern analysis and diagnosis is noted. The review considers a new trend in the development of bioanalytical methods using aptamers, multianalytical biochips, surface-enhanced Raman scattering systems, and bioelectroanalysis.<br> The bibliography includes 460 references.

show abstract

Highly Stable Mutant Bacterial Formate Dehydrogenase with Improved Catalytic Properties

Cited by 3 publications

References 18 publications

Formate Dehydrogenase: From Nad(p)h Regeneration To the Target in Pathogens Biofilms, a Component Of Highly Efficient Hybrid Biocatalysts and Co2 Fixation From the Atmosphere

Formate Dehydrogenase: From Nad(p)h Regeneration To the Target in Pathogens Biofilms, a Component Of Highly Efficient Hybrid Biocatalysts and Co2 Fixation From the Atmosphere

Formate Dehydrogenase: From NAD(P)H Regeneration to Targeting Pathogen Biofilms, Composing Highly Efficient Hybrid Biocatalysts and Atmospheric CO2 Fixation

Biocatalysis: modern problems and applications

Contact Info

Product

Resources

About