To determine a better combination of signs and markings on expressway tunnel entrance sections, three types of typical signs and markings were compared and tested according to five indicators: speed, lane lateral offset, lane change behavior, fixation behavior, and operating load, using a driving simulator. The results identified that the obvious no overtaking and speed limit signs, combined with a layer of thin red pavement, had the most influence on drivers’ speed, and they led to the highest fixation frequency of static facilities, the longest average distance from the completion point of the lane change to the entrance, and the longest average lane change distance, which could help drivers to pass through tunnel entrance sections more smoothly. The location of the static facilities should be between 3 s before the tunnel entrance and 3 s after entering the tunnel, as this is the area where a driver’s relative viewpoint changes. The improper combination of warning signs and deceleration measures will affect a driver’s judgment, causing negative effects, such as premature lane changes and an increased operating load. The research results can provide a design basis and reference for the combination setting of safety signs and markings on tunnel entrance sections.