Himatanthus drasticus (Apocynaceae) latex reduces oxidative stress and modulates CD4+, CD8+, FoxP3+ and HSP-60+ expressions in Sarcoma 180-bearing mice

Abstract

Treatment with HdCL reduced oxidative damage and modulated the expressions of CD4, CD8, FoxP3and HSP-60 in S180 solid tumor model, which can be associated to the presence of triterpenes, such as α-amyrin, β-amyrin and lupeol cinnamate. Present data emphasizes the importance of immune system in cancer and highlights the evaluation of the pharmacological properties of plants used by population as phytoterapics.

Structure identification, antitumor activity and mechanisms of a novel polysaccharide from Ramaria flaccida (Fr.) Quél

“…The present results indicated that Rplp1 was a key gene. RPLP1 interacts with the conservative regions of RPLP0, RPLP2 and 28S rRNA, which forms the main part of the ribosomal GTPase activity center, and can covalently bind to ubiquitin to form fusion proteins and participate in important biological activities ( 32 ), which was consistent with regulating cell apoptosis and transcription in the RF-1 group of the present study. The Rpl36 gene is directly involved in the synthesis of the ribosomal protein 50S subunit, while the Rpl34 gene belongs to the ribosomal protein L34E family, and is considered to serve an important role in apoptosis, and in the occurrence and development of various malignant tumors ( 33 , 34 ).…”

“…Additionally, there were upregulated genes with different FPKM in the blank control and RF-1 groups. The results revealed that 13 genes [cytochrome c oxidase subunit 1 (mt-Co1), ribosomal protein ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- lateral stalk subunit P1 (Rplp1), eukaryotic translation elongation factor 1 α 1 (Eef1a1), ribosomal protein L36 (Rpl36), ribosomal protein L34-pseudogene 1 (Rpl34-ps1), galectin 1 (Lgals1), mitochondrion Cytochrome b (mt-Cytb), predicted gene 12191 (Gm12191), NADH-ubiquinone oxidoreductase chain 1 (mt-Nd1), Gm13394, Rpl13, Rpl8 and Rps2-ps10] were markedly upregulated (FPKM>2,000) in the blank control group compared with in the RF-1 group (Table IV), and 13 genes (mt-Co1, Rplp1, Eef1a1, mt-Nd1, Lgals1, Rpl36, mt-Cytb, Gm13394, Gm12191, Rpl34-ps1, Rpl8, Rpl13 and Rps2-ps10) were markedly upregulated (FPKM>2,000) in the RF-1 group compared with in the blank control group ( (32), which was consistent with regulating cell apoptosis and transcription in the RF-1 group of the present study. The Rpl36 gene is directly involved in the synthesis of the ribosomal protein 50S subunit, while the Rpl34 gene belongs to the ribosomal protein L34E family, and is considered to serve an important role in apoptosis, and in the occurrence and development of various malignant tumors (33,34).…”

“…The inhibitory effect of a treatment on tumors is usually judged by the tumor size and the inhibitory rate of the treatment on the tumor. S180 cells are commonly used as cell lines to detect the antineoplastic activity of treatment in vivo ( 32 ). The results from the in vivo experiments are shown in Fig.…”

“…HMQC reflects the coupling association between directly connected 1 H and 13 C nuclei (31,32). After assigning the chemical shifts of the hydrogen nuclei in RF-1 via the 1 H-1 H COSY spectrum, the chemical shifts of the carbon nuclei can be assigned via the HMQC spectra.…”

Látex de plantas da família Apocynaceae: Uma revisão

“…Na Tabela 1 descrevemos as características referentes ao látex produzido pelas plantas pesquisadas, e informações vinculadas à forma de vida, preparação do látex (como o látex foi utilizado), compostos bioativos do metabolismo primário, constituintes do metabolismo secundário, atividades biológicas e referências. Moura et al, (2016), , Soares et al, (2008), Moura et al, (2020), Lucetti et al, (2010), Colares et al, (2008), Santos et al, (2018), Mousinho et al, (2011), .…”