Histamine, cortisol and organ changes in fatal anaphylactic shock in guinea-pigs

Hirvonen, J.; Huttunen, Pirkko

doi:10.1016/0379-0738(84)90089-6

Cited by 2 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, many neutrophils in pulmonary blood vessels, and additionally edema have also been reported in rabbits and monkeys 19,20 . Mast cells are considered as important for indicating changes of the heart and lungs, and for the death of animals as follows: increased plasma histamine levels and acute pulmonary emphysema have been seen only in dead guinea pigs sensitized and challenged by intracardial OA 21 ; degranulation of mast cells, increased heart rate, and decreased compliance and conductance of the lung have been reported in mice infused with goat anti-mouse IgG 22 . In the present study, therefore, vascular collapse is considered to be the main cause of death of the mice, and death was shown to be accompanied by decreased numbers of lymphocytes in the spleen, and the aforementioned increases in airway resistance and serum histamine levels with increased permeability of the subcutaneous microvasculature.…”

Section: Discussionmentioning

confidence: 99%

Development of a Novel Mouse Anaphylaxis Model Produced by Intermittent Intravenous Injections of Ovalbumin without Adjuvant

et al. 2007

View full text Add to dashboard Cite

Abstract:This study was performed to develop mouse anaphylaxis model using ovalbumin (OA) as an antigen, with no adjuvant, and an intravenous route of administration. OA at dose levels from 0.5 to 500 mg/kg was injected 3 times into the tail vein of female CD-1 (ICR) mice at 4-day intervals. Clinical signs and extravasation of Evans blue dye (vascular permeability) were observed, serum histamine levels and airway resistance were measured, and histopathological examinations were performed. Furthermore, to detect serum IgE and IgG, heterologous and homologous passive cutaneous anaphylaxis tests were performed. Additionally, strain differences in susceptibility to OA anaphylaxis was examined with 3 mouse strains: ICR, BALB/c and C57BL/6 mice. Shortly after the 3 rd injection, most of the animals exhibited systemic anaphylactic signs including death, and this was accompanied by increases in vascular permeability, plasma histamine levels, and airway resistance. Four days after the 2 nd injection, anti-OA IgE and IgG were detected in sera of mice injected with 0.5 and 5.0 mg/kg or higher doses of OA, respectively. Histological findings included: congestion in many tissues; detachment of mucosal epithelial cells in the gastrointestinal tract; enhanced death, decreased numbers of lymphocytes, and increased numbers of macrophages in the lymphoid tissues. ICR mice were shown to be most susceptible to the OA anaphylaxis of the 3 mouse strains examined. In conclusion, the results demonstrate that 3 intermittent intravenous injections of OA without adjuvant at 4-day intervals can induce anaphylactic shock in mice, in particular, most effectively in ICR mice, and that the anaphylaxis is characterized by specific IgE and IgG production. (J Toxicol Pathol 2007; 20: 237-244)

show abstract

Section: Discussionmentioning

confidence: 99%

Development of a Novel Mouse Anaphylaxis Model Produced by Intermittent Intravenous Injections of Ovalbumin without Adjuvant

et al. 2007

View full text Add to dashboard Cite

show abstract

“…One experiment showed high histamine values in guinea pigs that died rapidly of albumin anaphylaxis, but not in those animals in which death took 30 min [4]. The delay was considered to be an effect of histaminase, which inactivated the histamine liberated into the blood more slowly after intraperitoneal injection of the antigen than after intracardial injection.…”

Section: Introductionmentioning

confidence: 95%

Anaphylactic death: the effect of aminoguanidine and heparin on histamine and stress hormones in guinea pigs

et al. 1989

View full text Add to dashboard Cite

The modifying effect of aminoguanidine (a histaminase inhibitor) and heparin (a histaminase liberator) on anaphylactic shock in guinea pigs was studied using ovalbumin as an antigen and trigger. The animals died of the shock, the time to death remaining unaltered by the drugs. Serum histamine and cortisol values were high after shock, but were reduced by heparin. Both noradrenaline and adrenaline in plasma were also elevated after shock, the final concentration of the latter being lowered by heparin. The lungs were dilated, indicating bronchoconstriction. The results confirm the role of histamine in anaphylactic shock and its potential value for the diagnosis in this kind of rapid death, in which morphological signs are scarce or lacking. Its diagnostic value still requires confirmation, however, which only autopsy studies can supply. It also appears that pretreatment of the animals with heparin affected the blood cortisol and catecholamines, which are involved in the shock mechanism as countermeasures, although aminoguanidine did not have any effect.

show abstract

Histamine, cortisol and organ changes in fatal anaphylactic shock in guinea-pigs

Cited by 2 publications

References 7 publications

Development of a Novel Mouse Anaphylaxis Model Produced by Intermittent Intravenous Injections of Ovalbumin without Adjuvant

Development of a Novel Mouse Anaphylaxis Model Produced by Intermittent Intravenous Injections of Ovalbumin without Adjuvant

Anaphylactic death: the effect of aminoguanidine and heparin on histamine and stress hormones in guinea pigs

Contact Info

Product

Resources

About