Histamine H₄ receptors modulate dendritic cell migration through skin – immunomodulatory role of histamine

Abstract

Referring to DC migration, blocking the H4R on inflammatory cells might be a promising anti-inflammatory, immunomodulatory strategy.

International Union of Basic and Clinical Pharmacology. XCVIII. Histamine Receptors

“…Expression coupled with function defined by pharmacological methods has been shown on mast cells; eosinophils; neutrophils; dendritic cells; Langerhans cells; natural killer cells; monocytes; T cells, including gdT cells, T helper 1, 2, and 17 cells; keratinocytes; inflammatory dendritic epidermal cells; and fibroblasts (Gantner et al, 2002;Hofstra et al, 2003;Ling et al, 2004;Lippert et al, 2004;Gutzmer et al, 2005Gutzmer et al, , 2009Sander et al, 2006;Damaj et al, 2007;Dijkstra et al, 2007Dijkstra et al, , 2008Godot et al, 2007;Bäumer et al, 2008;Ikawa et al, 2008;Yamaura et al, 2009;Gschwandtner et al, 2010Gschwandtner et al, , 2011Gschwandtner et al, , 2012Gschwandtner et al, , 2013Truta-Feles et al, 2010;Mommert et al, 2012;Glatzer et al, 2013;Mirzahosseini et al, 2013;Cowden et al, 2014;Czerner et al, 2014;Dib et al, 2014;Jemima et al, 2014). In addition, expression data, but as to date no functional data, have been reported in epithelial cells and basophils (Hofstra et al, 2003;Sander et al, 2006).…”

“…Moreover, in contrast to the formyl peptide receptor, the H 4 receptor does not mediate reactive oxygen species formation or eosinophil peroxidase release (Reher et al, 2012). Dendritic cells migration can also be modulated by the H 4 receptor (Gutzmer et al, 2005;Damaj et al, 2007;Bäumer et al, 2008;Gschwandtner et al, 2010). Histamine-induced migration of dendritic cells in vitro or ex vivo and a H 4 R-dependent migration of dendritic cells was detected in vivo in mice during an allergic response (Cowden et al, 2010b).…”

Analysis of Histamine Receptor Knockout Mice in Models of Inflammation

“…Histamine-induced calcium mobilization and chemotaxis, both occurring in wild-type mast cells, were absent in mast cells derived from H 4 R 2/2 mice, while degranulation remained unaffected. The capacity of histamine to induce chemotaxis via the H 4 R was demonstrated using genetic and pharmacologic approaches for other cell types, such as eosinophils and dendritic cells (Thurmond et al, 2004;Bäumer et al, 2008;Cowden et al, 2010b;Gschwandtner et al, 2010;Simon et al, 2011;Reher et al, 2012b). In adaptive immunity, effects of the H 4 R seem to be limited to dendritic cell activation, both in terms of migration and mediator release, while data supporting a direct effect on T cells are ambiguous (Dunford et al, 2006;del Rio et al, 2012).…”

Histamine receptor expression in human renal tubules: a comparative pharmacological evaluation

“…The final concentration of the organic solvent was less than 0.1%, which had no effect on cell viability. Antibodies H 3 R and H 4 R were detected by using the anti-hH 3 R (349-358) and the anti-hH 4 R (374-390) antibodies produced and validated for detecting both human and rodent H 3 R and H 4 R in the School of Biological and Biomedical Sciences, Durham University [21][22][23][24][25][26][27]. Briefly, anti-hH 3 R antibody was generated coupling the peptide corresponding to the amino acids RLSRDRKVAK Cys of the human and rat H 3 R to thyroglobulin by m-maleimidobenzoic acid N-hydroxysuccinimide ester coupling method as described previously [6] and antibodies were raised in rabbits to the resultant conjugate.…”