Histobacteriological evaluation of advancing front of carious dentin: Excavation done with and without using caries disclosing dye - A comparative in-vitro study

Abstract

Background: Dental caries is the most common bacterial infection of the oral cavity. Early detection and complete removal of dental caries play a key role in the treatment and prognosis of dental caries. This study is aimed to evaluate histobacteriologically the advancing front of carious dentin: Excavation done with and without using caries disclosing dye. Materials and Methods: Freshly extracted 100 permanent teeth with coronal and proximal caries were collected in a saline solution under aseptic conditions. Group 1 (n = 50) caries were excavated using caries detecting dye 1% acid red solution in propylene glycol method. Group 2 (n = 50) caries were removed by visual and tactile method. These teeth specimen are decalcified, processed, and stained for bacterial evaluation. Results: The majority of the teeth (98%) in Group 1 showed the absence of bacteria, and only 10% of the teeth showed the absence of bacteria in Group 2. Conclusion: Caries detecting dye is a useful adjunct and can supplement the conventional methods of caries detection in assessing the extent of caries in dentin. light induced fluorescence. The above-mentioned techniques are either not reliable or far too expensive for all dentists to afford. In contrast to these aids, caries detector dyes are reliable, less expensive, and can be used effectively as a chair-side protocol. [2] The possibility of caries detector dyes was originally developed in the 1970's when basic fuchsin staining was used as a guide to the removal of the outer layer of infected unremineralized dentin in the carious lesion. There are many dyes such as methyl red, alizarin stain, 8-hydroxyquinoline, fluorescent dyes, carbolan green, coomassie blue, lissamine blue, 0.5% basic fuchsin, 1.0% acid red, which have been used for detecting caries, and was used to help the clinician to distinguish between affected and infected dentin during cavity preparation. There has always been concern about the safety of disclosing agents in terms of carcinogenicity, and 1% acid red in propylene glycol was introduced as a safe and effective alternative. [2-4]