Histochemical and transmission electron microscopic studies of spleen in prenatal stages of goat

Gautam, A. K.; Mishra, U. K.

doi:10.5958/0976-0555.2015.00013.8

Cited by 2 publications

(4 citation statements)

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“…It’s necessary for the physiological function and development of the spleen. Gautam and Mishra (2015) reported that red pulp in the spleen of goats showed weak or moderate activity to AB stain, similar to our findings in hamsters, and mentioned that intensity increases with age advancement. This study has a limitation in its scope of examination, as it only covers a subset of morphological parameters, particularly at the cellular level, and does not incorporate the use of other histochemical markers.…”

Section: Discussionsupporting

confidence: 89%

“…The examination of the spleens’ histochemistry revealed an intense reaction to PAS stain in capsules, trabecula. At the wall of splenic blood vessels, these findings were parallel to those ( Gautam and Mishra, 2015 , Kaur et al , 2022 ) mentioned that glycoprotein components in these structures were high. It’s necessary for the physiological function and development of the spleen.…”

Section: Discussionsupporting

confidence: 81%

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Morphological, histological, and histochemical study of the adult golden hamster (Mesocricetus auratus) spleen

Hasan¹,

Altaey²,

Sultan³

2023

Open Vet J

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Background: The golden hamster is a choice model for investigating many visceral and splenic infections and neoplastic and retrospective lesions. Aim: To study hamsters' spleen's morphological, histological, and histochemical structure. Methods: Samples were collected from eight healthy adult golden hamsters and then fixed with 10% buffered formalin. Later, samples were processed, sectioned, and stained with Hematoxylin and Eosin as well as Masson’s Trichrome stain. Other slides were further stained with Periodic Acid Schiff and Alcian blue 2.5 stain (PAS) for histochemical evolution; the gross measurement was performed for the splenic length, width, and thickness, while the histological measures included the splenic capsular and trabecula thickness, diameter of white pulp follicles, splenic sinusoids and central arteries and proportion of white and red pulps. Results: The macroscopic findings revealed that the spleen was red-brown lanciform on the left side of the dorsolateral abdominal wall. The morphological measurements for splenic length, width, and thickness were 26.6 ± 7.67, 4.17 ± 1.65, and 1.70 ± 0.01 mm, respectively. The histological observations showed that the splenic capsule was composed of two layers (serosal and subserosal). The inner layer sends trabeculae dividing the splenic parenchyma irregularly, and the splenic parenchyma comprises the white and red pulp. The white pulp follicles included the mantle, marginal zones, and the PALS (periarterial lymphatic sheath), while the red pulp constituted splenic cords and sinuses. The histomorphological findings showed that white pulp follicles and the central artery mean diameter were 252.62 ± 8.07 µm and 54.45 ± 0.36 µm respectively, the proportion of white to a red pulp was 0.49 ± 0.01, the splenic capsule, trabecula and the wall of splenic arteries showed an intense positive activity to PAS stain and negative or weak in other splenic structures. Conclusion: The similarities and differences in the spleen between the laboratory animals and hamsters were apparent in this article, so understanding the morphological and histological structure of the spleen presents significant assistance with species identification to select the appropriate experimental animal model in future medical research

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 81%

Morphological, histological, and histochemical study of the adult golden hamster (Mesocricetus auratus) spleen

Hasan¹,

Altaey²,

Sultan³

2023

Open Vet J

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“…Pig (Sangild et al, 2000), Buffalo (Singh et al, 2009) and in Camel (Bello et al, 2012), but very little systematic attention has been paid towards early prenatal development of the intestine in goats (Chima & Nwagbo, 2009;Gautam & Mishra, 2016;Raghav, 2016;Verma et al, 2020;Verma, Farooqui, Prakash, Pathak, Singh, Gupta, & Goyal, 2021;Verma, Farooqui, Prakash, Pathak, Singh, Gupta, Kumar, et al, 2021;Verma, Farooqui, Prakash, Pathak, Singh, Yadav, et al, 2021). Beside this, the information collected in the present study will also fulfil a gap in the scientific literature of prenatal development in goats.…”

mentioning

confidence: 79%

“…The development of the intestine is important for the adult gastroenterologist to appreciate, because of the potential for these early life events to affect the responsiveness of the intestine to physiological or pathological challenges later in life (Drozdowski et al, 2010). Prenatal development of intestine has been studied in Sheep (Karadag et al, 1994), Pig (Sangild et al, 2000), Buffalo (Singh et al, 2009) and in Camel (Bello et al, 2012), but very little systematic attention has been paid towards early prenatal development of the intestine in goats (Chima & Nwagbo, 2009; Gautam & Mishra, 2016; Raghav, 2016; Verma et al, 2020; Verma, Farooqui, Prakash, Pathak, Singh, Gupta, & Goyal, 2021; Verma, Farooqui, Prakash, Pathak, Singh, Gupta, Kumar, et al, 2021; Verma, Farooqui, Prakash, Pathak, Singh, Yadav, et al, 2021). Beside this, the information collected in the present study will also fulfil a gap in the scientific literature of prenatal development in goats.…”

Section: Introductionmentioning

confidence: 99%

Embryonic differentiation of intestine in Indian goats (Capra hircus)

Verma

Farooqui

Prakash

et al. 2023

Anat Histol Embryol

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The present study focused on identifying knowledge gaps in scientific literature for developmental anatomy and future fetal‐programming researches on goats. The aim of this study was to observe the sequential growth and the development of the intestine from stage of formation of preliminary digestive tube to the differentiation of various segments during early prenatal life in Indian goats. The developing digestive tube was first witnessed on the 23rd day of gestation histologically and from 25 days of gestation onwards, it became observable grossly under stereozoom microscope. The further stages of developing intestine rotation, herniation and coiling and re‐entry stages were noticed on the 38th day, 41st day and 48th day of gestation, respectively. The first demarcation between small and large intestines in the form of cecal bulge was recorded at 41st day of gestation. On the 45th day of gestation, all the segments of intestine that is, duodenum, jejunum, ileum, caecum, colon and rectum were well demarcated. Microscopically, the wall of the digestive tube was composed of three layers; lamina epithelialis, pleuripotent blastemic tissue and the mesothelium in between 23 and 39 days of gestation. At 41st day of gestation, the blastemic tissue showed distinct separation into lamina propria‐submucosa and tunica muscularis. Among connective tissue fibres only reticular fibres were observable in this study. The histochemical localisation of polysaccharides, bound lipids and alkaline phosphatase enzyme showed mild to moderate reaction. The reaction for acid phosphatase enzyme could not be observed or was absent in this study.

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Histochemical and transmission electron microscopic studies of spleen in prenatal stages of goat

Cited by 2 publications

References 0 publications

Morphological, histological, and histochemical study of the adult golden hamster (Mesocricetus auratus) spleen

Morphological, histological, and histochemical study of the adult golden hamster (Mesocricetus auratus) spleen

Embryonic differentiation of intestine in Indian goats (Capra hircus)

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