Histochemical studies on gut associated lymphoid tissue of pig (&lt;em&gt;Sus scrofa&lt;/em&gt;)

Urmila, T Sai; Ramayya, P. Jagapathi; Lakshmi, M. S.; Kumar, Avn Siva

doi:10.22271/chemi.2020.v8.i1a.8295

Cited by 2 publications

(1 citation statement)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 2021 ; Urmila et al. 2019 ), some locations such as the oesophagus, gallbladder and urinary bladder are not as frequent.…”

Section: Discussionmentioning

confidence: 99%

Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus

Porras,

Sánchez-Vizcaíno,

Rodríguez-Bertos

et al. 2024

Veterinary Quarterly

View full text Add to dashboard Cite

Despite the great interest in the development of a vaccine against African swine fever (ASF) in wild boar, the immunological mechanisms that induce animal protection are still unknown. For this purpose, tertiary lymphoid organs (TLOs) of wild boar were characterised and compared with mucosa-associated lymphoid tissues (MALTs) by histopathology, histomorphometry and immunohistochemistry (CD3, CD79, PAX5, LYVE1, fibronectin). In addition, real-time polymerase chain reaction (qPCR) and immunohistochemistry (p72) were used to evaluate the presence of ASF virus (ASFV) in blood and tissues samples, respectively. TLOs were observed in animals infected with a low-virulent ASFV isolate (LVI), animals co-infected with low and high-virulent ASFV isolates (LVI-HVI) and animals infected only with the high virulence isolate (HVI). TLOs in LVI and LVI-HVI groups were located adjacent to the mucosa and presented a similar structure to MALT. Immunoexpresion of p72 observed in the inflammatory cells adjacent to TLOs/MALTs confirmed its development and reactivity generated by ASF attenuated isolates. Immunohistochemical evaluation, based on cellular composition (T and B lymphocytes), and histomorphometrical study revealed a more pronounced maturation of TLOs/MALTs in the LVI-HVI group. It is currently unclear whether these formations play a protective role by contributing to local immunity in chronic inflammatory diseases. However, the structural similarities between TLOs and MALTs and the location of TLOs close to the mucosa suggest that they may perform a similar function, facilitating a local protective response. Nevertheless, further investigations are warranted to assess the cellular and humoral dynamics of these lymphoid organs induced by attenuated isolates.

show abstract

“… 2021 ; Urmila et al. 2019 ), some locations such as the oesophagus, gallbladder and urinary bladder are not as frequent.…”

Section: Discussionmentioning

confidence: 99%

Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus

Porras,

Sánchez-Vizcaíno,

Rodríguez-Bertos

et al. 2024

Veterinary Quarterly

View full text Add to dashboard Cite

show abstract

Age-dependent distribution and size of lymphatic nodules as components of extrahepatic bile duct-associated lymphatic tissue in domestic swine - a micromorphometric study

Stefanov

2023

BJVM

View full text Add to dashboard Cite

Due to the biological and immunological similarity between pigs and humans, pigs are used in medical research. Many morphological studies regarding the structure of the components of mucosal associated lymphatic tissue have been performed in animals including pigs as well as in humans. However, no any information about the existence of extrahepatic bile duct-associated lymphoid tissue (EHBDALT) is available in pigs. The aim of this work was to study the distribution and size of lymphatic nodules in the wall of extrahepatic bile ducts of immature and mature pigs in order to describe the structure of EHBDALT as component of mucosa-associated lymphoid tissue. It was found out that EHBDALT consisted of diffuse lymphatic tissue, solitary and aggregated lymphatic nodules. In 2-month-old pigs, diffuse lymphatic tissue predominated but single lymphatic nodules were found as inactive homogenous encapsulated aggregates of lymphocytes. In mature animals, diffuse lymphatic tissue, primary and secondary nodules were observed. The present micromorphometric study allowed evaluating the age-dependent distribution and size of lymphatic nodules in porcine extrahepatic bile ducts. It provides original data on the presence and age-dependent structure of bile duct MALT in domestic pigs.

show abstract

Histochemical studies on gut associated lymphoid tissue of pig (<em>Sus scrofa</em>)

Cited by 2 publications

References 4 publications

Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus

Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus

Age-dependent distribution and size of lymphatic nodules as components of extrahepatic bile duct-associated lymphatic tissue in domestic swine - a micromorphometric study

Contact Info

Product

Resources

About