Histocompatibility Assessment in the Chicken Colonies of the RIR-Y8/NU, YL, WL-G, and BL-E Closed for 28-48 Years

Valdez, Marcos B.; Kinoshita, Keiji; Mizutani, Makoto; Fujiwara, Akira; Yazawa, Hajime; Yamagata, T.; Shimada, Kiyoshi; Namikawa, Takao

doi:10.1538/expanim.58.169

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…Japanese quails were a commercial line purchased from local hatcheries for groups A–E (Kato-farm, Toyohashi, Japan) and F–M (Motoki Corporation, Saitama, Japan), and used at 2-to-12 mo old. To collect chicken semen, adult male chickens of Ehimejidori (Japanese native chicken breed) 59 , the NH-413 line (dystrophic New Hampshire chickens, line 413) 60 , the BL-E line (long-term closed colony of Brown Leghorn breed) 61 , and the GSP line (inbred line of Fayoumi breed) 62 were used. These chicken breeds and lines are maintained at the Avian Bioscience Research Center, Nagoya University.…”

Section: Methodsmentioning

confidence: 99%

Embryonic development and inviability phenotype of chicken-Japanese quail F1 hybrids

Ishishita

Kinoshita

Nakano

et al. 2016

Sci Rep

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Interspecific hybrid incompatibility, including inviability and sterility, is important in speciation; however, its genetic basis remains largely unknown in vertebrates. Crosses between male chickens and female Japanese quails using artificial insemination can generate intergeneric hybrids; however, the hatching rate is low, and hatched hybrids are only sterile males. Hybrid development is arrested frequently during the early embryonic stages, and the sex ratio of living embryos is male-biased. However, the development and sex ratio of hybrid embryos have not been comprehensively analyzed. In the present study, we observed delayed embryonic development of chicken-quail hybrids during the early stage, compared with that of chickens and quails. The survival rate of hybrids decreased markedly during the blastoderm-to-pre-circulation stage and then decreased gradually through the subsequent stages. Hybrid females were observed at more than 10 d of incubation; however, the sex ratio of hybrids became male-biased from 10 d of incubation. Severely malformed embryos were observed frequently in hybrids. These results suggest that developmental arrest occurs at various stages in hybrid embryos, including a sexually non-biased arrest during the early stage and a female-biased arrest during the late stage. We discuss the genetic basis for hybrid inviability and its sex bias.

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Section: Methodsmentioning

confidence: 99%

Embryonic development and inviability phenotype of chicken-Japanese quail F1 hybrids

Ishishita

Kinoshita

Nakano

et al. 2016

Sci Rep

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“…Red jungle fowls in Kagoshima, Japan were kept in a zoological garden. The birds in Aichi were reared for academic research in closed colonies or inbred lines at the Avian Bioscience Research Center (ABRC) of Nagoya University (Shimada et al, 2007;Valdez et al, 2009). The birds in South Korea were kept in a zoological garden.…”

Section: Methodsmentioning

confidence: 99%

Epidemiological study of fowl glioma-inducing virus in chickens in Asia and Germany

et al. 2012

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Fowl glioma-inducing virus (FGV), which belongs to avian leukosis virus (ALV) subgroup A, induces fowl glioma. This disease is characterized by multiple nodular gliomatous growths of astrocytes and has been previously reported in Europe, South Africa, Australia, the United States and Japan. FGV and FGV variants have spread to ornamental Japanese fowl, including Japanese bantams (Gallus gallus domesticus), in Japan. However, it is unclear how and where FGV emerged and whether FGV is related to the past fowl glioma in European countries. In this study, the prevalence of FGV in European, Asian and Japanese native chickens was examined. FGV could not be isolated from any chickens in Germany and Asian countries other than Japan. Eighty (26%) out of 307 chickens reared in Japan were positive by FGV-screening nested polymerase chain reaction and 11 FGV variants with an FGV-specific sequence in their 3? untranslated region were isolated. In addition, four other ALVs lacking the FGV-specific sequence were isolated from Japanese bantams with fowl glioma and/or cerebellar hypoplasia. These isolates were considered to be distinct recombinant viruses between FGV variants and endogenous/exogenous avian retroviruses. These results suggest that the variants as well as distinct recombinant ALVs are prevalent among Japanese native chickens in Japan and that FGV may have emerged by recombination among avian retroviruses in the chickens of this country.

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“…Commercial quail were purchased from a local hatchery (Cyubu Kagaku Shizai, Nagoya, Japan), and fertilized chicken eggs of the Ehime-jidori (Japanese native chicken breed) [55] and the BL-E line (long-term closed colony of Brown Leghorn breed) [56] were supplied by the National BioResource Project Chicken/Quail, Nagoya University, Japan. For chromosome analysis, we used embryos of commercial quail and Ehime-jidori chickens and their F 1 hybrid embryos that were obtained by AI of semen from male Ehime-jidori chickens into female quail.…”

Section: Animalsmentioning

confidence: 99%

Transcriptome analysis revealed misregulated gene expression in blastoderms of interspecific chicken and Japanese quail F1 hybrids

et al. 2020

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Hybrid incompatibility, such as sterility and inviability, prevents gene flow between closelyrelated populations as a reproductive isolation barrier. F 1 hybrids between chickens and Japanese quail (hereafter, referred to as quail), exhibit a high frequency of developmental arrest at the preprimitive streak stage. To investigate the molecular basis of the developmental arrest at the preprimitive streak stage in chicken-quail F 1 hybrid embryos, we investigated chromosomal abnormalities in the hybrid embryos using molecular cytogenetic analysis. In addition, we quantified gene expression in parental species and chicken-and quail-derived allele-specific expression in the hybrids at the early blastoderm and preprimitive streak stages by mRNA sequencing. Subsequently, we compared the directions of change in gene expression, including upregulation, downregulation, or no change, from the early blastoderm stage to the preprimitive streak stage between parental species and their hybrids. Chromosome analysis revealed that the cells of the hybrid embryos contained a fifty-fifty mixture of parental chromosomes, and numerical chromosomal abnormalities were hardly observed in the hybrid cells. Gene expression analysis revealed that a part of the genes that were upregulated from the early blastoderm stage to the preprimitive streak stage in both parental species exhibited no upregulation of both chicken-and quail-derived alleles in the hybrids. GO term enrichment analysis revealed that these misregulated genes are involved in various biological processes, including ribosome-mediated protein synthesis and cell proliferation. Furthermore, the misregulated genes included genes involved in early embryonic development, such as primitive streak formation and gastrulation. These results suggest that numerical chromosomal abnormalities due to a segregation failure does not cause the lethality of chicken-quail hybrid embryos, and that the downregulated expression of the genes that are involved in various biological processes, including translation and primitive streak formation, mainly causes the developmental arrest at the preprimitive streak stage in the hybrids.

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Histocompatibility Assessment in the Chicken Colonies of the RIR-Y8/NU, YL, WL-G, and BL-E Closed for 28-48 Years

Cited by 5 publications

References 14 publications

Embryonic development and inviability phenotype of chicken-Japanese quail F1 hybrids

Embryonic development and inviability phenotype of chicken-Japanese quail F1 hybrids

Epidemiological study of fowl glioma-inducing virus in chickens in Asia and Germany

Transcriptome analysis revealed misregulated gene expression in blastoderms of interspecific chicken and Japanese quail F1 hybrids

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