Histologic Response and Tenascin and Fibronectin Expression After Pulp Capping in Pig Primary Teeth With Mineral Trioxide Aggregate or Calcium Hydroxide

Abstract

This study evaluated the histological response and the expression of tenascin (TN) and fibronectin (FN) after pulp capping with mineral trioxide aggregate (MTA) or calcium hydroxide (CH). Class V cavities and pulp exposure were performed in 40 primary pig teeth. The pulps were capped with either MTA or CH, and the cavities were sealed with resin-modified glass ionomer cement. CH was used as a control. Seven and 70 days posttreatment, the animals were sacrificed and teeth were prepared for histological evaluation. TN and FN were detected by immunostaining. A severe inflammatory response was observed after 7 days in the CH group (p<0.043), while in the MTA group, a mild response was observed. Similar reparative dentin deposition was observed after 70 days for both groups (p<0.005). The expression of FN and TN was similar for both groups in the two periods evaluated. TN and FN were expressed during pulp reparative events, independently of the capping material.

Novel experimental cements for use on the dentin-pulp complex

“…Other investigations have demonstrated improved mechanical and biological properties for MTA when compared with calcium hydroxide cements (5,6). The superior biological responses of MTA have been attributed to the higher solubilization of growth factors from dentin with MTA, although other mechanisms may also contribute to its performance.…”

“…Mineral trioxide aggregate (MTA) has also found wide use as a pulp-capping agent (5). Other investigations have demonstrated improved mechanical and biological properties for MTA when compared with calcium hydroxide cements (5,6).…”

Tertiary dentinogenesis with calcium hydroxide: A review of proposed mechanisms

“…, Leites et al . ). Another in vivo study conducted on dog dental pulp tissue to determine immunolocalization of fibronectin after interaction of pulp with Ca(OH) 2 ‐containing material showed that fibronectin had a strong association with microcrystals formed at the surface of the cement (Tziafas et al .…”

“…Expression of fibronectin has been demonstrated in vivo in dental pulps following pulp capping with Ca(OH) 2 (Yoshiba et al 1996, Piva et al 2006, Fernandes et al 2008, Leites et al 2011. Another in vivo study conducted on dog dental pulp tissue to determine immunolocalization of fibronectin after interaction of pulp with Ca(OH) 2 -containing material showed that fibronectin had a strong association with microcrystals formed at the surface of the cement (Tziafas et al 1995b).…”

MTA in Vital Pulp Therapy

“…It has been proposed that glycoproteins in the extracellular matrix can stimulate the formation of reparative or reactionary dentin (Smith et al 1995;Goldberg & Smith 2004;Goldberg et al 2008). In particular, tenascin and fibronectin have been identified as high molecular weight oligomeric proteins involved in odontoblastic differentiation and odontogenesis in the presence of MTA (Thesleff et al 1995;Leites et al 2011;Zarrabi et al 2011). Both glycoproteins are expressed during dentinogenesis and may be critical elements in pulp cell migration and differentiation (Zarrabi et al 2011).…”