Histological and biochemical apoptosis changes of female rats′ ovary by Zinc oxide nanoparticles and potential protective effects of l-arginine: An experimental study

Efendic, Fatima; Sapmaz, Tansel; Canbaz, Halime Tuba; Pençe, Halime Hanım; İrkörücü, Oktay

doi:10.1016/j.amsu.2022.103290

Cited by 9 publications

(2 citation statements)

References 28 publications

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“… 33 However, increased atresia can result in impaired ovarian function. One study reported that atretic follicles in the ovaries of rats increased gradually with the increase in ZnO NPs dosage, 34 which showed a pattern similar to the results of our study. Follicular development and maturation are accompanied by the secretion of sex hormones.…”

Section: Discussionsupporting

confidence: 90%

Zinc Oxide Particles Can Cause Ovarian Toxicity by Oxidative Stress in Female Mice Model

Xu¹,

Zhao²,

Liu³

et al. 2022

IJN

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Introduction Zinc oxide nanoparticles (ZnO NPs) participate in all aspects of our lives, but with their wide application, more and more disadvantages are exposed. The goal of this study was to investigate the toxicity of ZnO NPs in female mice ovaries and explore its potential mechanism. Methods In this study, adult female mice were orally exposed to 0, 100, 200, and 400 mg/kg ZnO NPs for 7 days. We explored the underlying mechanisms via the intraperitoneal injection of N-acetyl-cysteine (NAC), an inhibitor of oxidative stress, and salubrinal (Sal), an inhibitor of endoplasmic reticulum (ER) stress. Results The results indicated that serum estradiol and progesterone levels declined greatly with increasing ZnO NPs dosage. Hematoxylin and eosin (HE) staining revealed increased atretic follicles and exfoliated follicular granulosa cells. Moreover, at the transcriptional level, antioxidant-related genes such as Keap1 and Nrf2 , and ER stress-related genes PERK, eIF2α , and ATF4 were markedly upregulated. In addition, the expression of Caspase12, Caspase9 , and Caspase3 , which are genes related to apoptosis, was also upregulated in all ZnO NPs treatment groups. Serum malondialdehyde (MDA) content was remarkably up-regulated, whereas superoxide dismutase (SOD) activity was down-regulated. The 400 mg/kg ZnO NPs treatment group suffered the most substantial harm. However, ovarian damage was repaired when NAC and Sal were added to this group. Conclusion ZnO NPs had toxic effects on the ovary of female mice, which were due to oxidative stress, ER stress, and the eventual activation of apoptosis.

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Section: Discussionsupporting

confidence: 90%

Zinc Oxide Particles Can Cause Ovarian Toxicity by Oxidative Stress in Female Mice Model

Xu¹,

Zhao²,

Liu³

et al. 2022

IJN

View full text Add to dashboard Cite

show abstract

“…The importance of intracellular zinc levels for follicle health and maturation has been established ( 16 , 18-21 ). Zinc influx is associated with increased expression of apoptosis-related genes ( 21 ) as well as those related to cumulus expansion, inflammation, hormone signaling, and steroidogenesis in granulosa cells ( 19 , 20 ). Apart from its zinc transporter function, SLC39A9 can couple with G-proteins ( 22 ).…”

Section: Androgens Act Through Ar and Slc39a9mentioning

confidence: 99%

Novel Plasma Membrane Androgen Receptor SLC39A9 Mediates Ovulatory Changes in Cells of the Monkey Ovarian Follicle

Sage,

Duffy

2024

Endocrinology

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Follicular androgens are important for successful ovulation and fertilization. The classical nuclear androgen receptor (AR) is a transcription factor expressed in the cells of the ovarian follicle. Androgen actions can also occur via membrane androgen receptor SLC39A9. Studies in fish ovary demonstrated that androgens bind to SLC39A9 and increase intracellular zinc to regulate ovarian cell function. To determine if SLC39A9 is expressed and functional in the key cell types of the mammalian ovulatory follicle, adult female cynomolgus macaques underwent ovarian stimulation. Ovaries or ovarian follicular aspirates were harvested at 0, 12, 24, and 36 hours after human chorionic gonadotropin (hCG). SLC39A9 and AR mRNA and protein were present in granulosa, theca, and vascular endothelial cells across the entire 40-hour ovulatory window. Testosterone, bovine serum albumin–conjugated testosterone (BSA-T), and androstenedione stimulated zinc influx in granulosa, theca, and vascular endothelial cells. The SLC39A9-selective agonist (−)-epicatechin also stimulated zinc influx in vascular endothelial cells. Taken together, these data support the conclusion that SLC39A9 activation via androgen induces zinc influx in key ovarian cells. Testosterone, BSA-T, and androstenedione each increased proliferation in vascular endothelial cells, indicating the potential involvement of SLC39A9 in ovulatory angiogenesis. Vascular endothelial cell migration also increased after treatment with testosterone, but not after treatment with BSA-T or androstenedione, suggesting that androgens stimulate vascular endothelial cell migration through nuclear AR but not SLC39A9. The presence of SLC39A9 receptors and SLC39A9 activation by follicular androstenedione concentrations suggests that androgen activation of ovarian SLC39A9 may regulate ovulatory changes in the mammalian follicle.

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The antioxidant and anti-apoptotic properties of vitamins A, C and E in heart tissue of rats exposed to zinc oxide nanoparticles

et al. 2022

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Histological and biochemical apoptosis changes of female rats′ ovary by Zinc oxide nanoparticles and potential protective effects of l-arginine: An experimental study

Cited by 9 publications

References 28 publications

Zinc Oxide Particles Can Cause Ovarian Toxicity by Oxidative Stress in Female Mice Model

Zinc Oxide Particles Can Cause Ovarian Toxicity by Oxidative Stress in Female Mice Model

Novel Plasma Membrane Androgen Receptor SLC39A9 Mediates Ovulatory Changes in Cells of the Monkey Ovarian Follicle

The antioxidant and anti-apoptotic properties of vitamins A, C and E in heart tissue of rats exposed to zinc oxide nanoparticles

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