Histological and histochemical study on mammary gland of Damascus goats through stages of lactation

El-Sayed, Eitedal H.; El-Shafie, M. H.; Saifelnasr, E. O. H.; El-Ella, A. A. Abu

doi:10.1016/j.smallrumres.2009.06.019

Cited by 8 publications

(7 citation statements)

References 15 publications

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“…Indeed, development of the lobular-alveolar system is obviously essential to produce and store milk, which replaces stroma, reducing its amount in the mammary gland, whereas the alteration of the epithelial cells to a columnar shape is probably due to their proliferation. More recently, Elsayed et al (2009) studied mammary gland patterns throughout lactation in Damascus goats, also observing an increase in alveolar secretory cells number during early and mid-lactation and a diminishment in late lactation. Likewise, the alveoli area was smaller during late lactation compared with early and mid-stages.…”

Section: Mammary Gland Morphology During Lactation: Macroscopic and Mmentioning

confidence: 99%

The mammary gland in small ruminants: major morphological and functional events underlying milk production – a review

Lérias

Hernández-Castellano

Suárez-Trujillo

et al. 2014

Journal of Dairy Research

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The importance of small ruminants to the dairy industry has increased in recent years, especially in developing countries, where it has a high economic and social impact. Interestingly and despite the fact that the mammary gland is the specialised milk production organ, very few authors studied the modifications occurring in the mammary gland through the lactation period in production animals, particularly in the small ruminants, sheep (Ovis aries) and goat (Capra hircus). Nevertheless, understanding the different mammary gland patterns throughout lactation is essential to improve dairy production. In addition, associating these patterns with different milking frequencies, lactation number or different diets is also of high importance, directly affecting the dairy industry. The mammary gland is commonly composed of parenchyma and stroma, which includes the ductal system, with individual proportions of each changing during the different periods and yields in a lactation cycle. Indeed, during late gestation, as well as during early to mid-lactation, mammary gland expansion occurs, with an increase in the number of epithelial cells and lumen area, which leads to increment of the parenchyma tissue, as well as a reduction of stroma, corresponding macroscopically to the increase in mammary gland volume. Throughout late lactation, the mammary gland volume decreases owing to the regression of the secretory structure. In general, common mammary gland patterns have been shown for both goats and sheep throughout the several lactation stages, although the number of studies is limited. The main objective of this manuscript is to review the colostrogenesis and lactogenesis processes as well as to highlight the mammary gland morphological patterns underlying milk production during the lactation cycle for small ruminants, and to describe potential differences between goats and sheep, hence contributing to a better description of mammary gland development during lactation for these two poorly studied species.

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Section: Mammary Gland Morphology During Lactation: Macroscopic and Mmentioning

confidence: 99%

The mammary gland in small ruminants: major morphological and functional events underlying milk production – a review

Lérias

Hernández-Castellano

Suárez-Trujillo

et al. 2014

Journal of Dairy Research

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“…The weak to negligible activity of protein in tissue sections of mastitic animal in the present study could be due to degenerative changes of mammary epithelium with connective tissue proliferation and impaired activity of endoplasmic reticulum induced by microbial agents. However, different researchers reported that protein staining was decreased (Elsayed et al, 2009 and. These degenerated mammary cells encompassing the active cellular protein was substituted by the spread of connective tissue under the effect of P. multocida which results in poor biosynthetic capacity of udder and decreases cellular differentiation (Hussain et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Advances., 1 (2): 98-100 Elsayed, E.H.;EL-Shafie, M.H. ;Saifelnasr, E.O.H. and Abu El-Ella A.A. (2009)…”

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confidence: 99%

Some Studies on Pasteurella Multocida as a Causative Agent of Mastitis in Dairy Cows and Ewes

Asfour¹,

Anwer²,

Nabeeh³

et al. 2016

Assiut Veterinary Medical Journal

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A total of 233 mastitic milk samples (151 cows and 82 ewes) and 90 mammary gland tissue samples (56 cows and 34 ewes) were collected from different Egyptian Governorates for P. multocida investigation as one of mastitis causing pathogens and its effect on the mammary gland tissues. The isolated P. multocida from clinical mastitic milk was slightly higher than that from subclinical form in both cows and ewes (15.3% versus 13.1% and 27.3% versus 26.7%, respectively) and it was isolated from the udder tissues of both cows and ewes with percentages of (17.85%) and (23.52%), respectively. Enzyme linked immunosorbent assay (ELISA) was used as confirmatory, rapid and reliable test for detection of P. multocida antibodies in the tested milk samples and they were detected in mastitic cow's and ewe's milk whey in (17.2%) and (34.2%), respectively. The antibiogram profile of P. multocida was studied for detection of its susceptibility to 15 different antibiotics to detect the drug of choice for its treatment. P. multocida isolated from cow's milk showed more resistance to various antibiotics than that isolated from ewe's milk. The DNA integrity of mammary gland tissue cells was detected using comet assay and the percentage of DNA damage was significantly elevated in case of P. multocida infected mammary gland (P<0.05). In addition, the histopathological findings of P. multocida infected udder showed focal and/or diffuse chronic lymphocytic mastitis with an extensive degeneration and necrosis of the alveolar epithelium as well as interstitial tissue. Most of the mammary alveoli were filled with basophilic bacterial colonies with bipolar bodies positively stained by methylene blue and Giemsa stains. Cytological evaluation was conducted on all udder tissue samples and 16/18 positive cases (88.8%) were correlated with their histopathological examination. Histochemically, tissue sections from infected udder showed weak or no alkaline phosphatase activity and density of protein staining. It was concluded that P. multocida should be considered as an important sharing etiological agent of mastitis in both cows and ewes (especially in ewes) and associated with significant histopathological alterations in the glandular tissue structure. ELISA was considered as a quick and reliable technique for detection of P. multocida infection in the mammary gland especially in the un-vaccinated farms beside the traditional cultural method. The cytological interpretation was quiet helpful in rapid screening of the mammary gland affections.

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“…In the declining lactation, a gradual involution due to an entirely reduced cell number is observed, and this occurs more rapidly after milking ceases (Knight and Wilde 1993). ELsayed et al (2009) observed, in sections of goats' mammary gland tissues, that the histological structure showed clear differences between lactation stages; being more developed at early and mid-stages, compared to late-stage of lactation.…”

Section: Introductionmentioning

confidence: 90%

Effects of breed and milking frequency on udder histological structures in dairy goats

Suárez-Trujillo

Capote²,

Argüello

et al. 2013

Journal of Applied Animal Research

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Tissue percentages (secretory, connective, ductal and vascular tissues) and the number and size of the alveoli in the udders of three dairy goat breeds under two milking frequencies (once-vs. twice-daily milking) were studied. Nine dairy goats, three of each breed studied (Majorera, Tinerfen˜a and Palmera), were milked during 6 weeks beginning at the tenth week of lactation. The right udder half was milked twice daily, and the left udder half was milked once daily. Moreover, during the experimental period, morphological udder data, milk yield, milk fractioning and milk composition were recorded. The goats were sacrificed and two samples for each gland were taken for the histological study. The statistical analyses revealed that the histological parameters were not influenced by the milking frequency, and that the breed determines different percentage of tissue components. Correlations between morphological parameters of the udder and milk-yielding parameters were high and determined the greater importance of the globosity and the structure of the udder in the milk production. Furthermore, it was determined that the percentage of secretory tissue in the mammary parenchyma has no correlation with the milk yield parameters in different high-production dairy breeds. Histological parameters (secretory and connective tissues) only have an impact in the milk fractions.

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Histological and histochemical study on mammary gland of Damascus goats through stages of lactation

Cited by 8 publications

References 15 publications

The mammary gland in small ruminants: major morphological and functional events underlying milk production – a review

The mammary gland in small ruminants: major morphological and functional events underlying milk production – a review

Some Studies on Pasteurella Multocida as a Causative Agent of Mastitis in Dairy Cows and Ewes

Effects of breed and milking frequency on udder histological structures in dairy goats

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