Histological and histomorphometrical comparative study of β-tricalcium phosphate block grafts and periosteal expansion osteogenesis for alveolar bone augmentation

Yamauchi, Kensuke; Takahashi, Tsuneo; Funaki, Katsuyuki; Hamada, Yuko; Yamashita, Yuichi

doi:10.1016/j.ijom.2010.05.008

Cited by 38 publications

(29 citation statements)

References 27 publications

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“…Therefore, currently employed reabsorbable materials in periodontal therapy (e.g. nano/micro-sized calcium phosphate, hydroxyapatite or beta-tricalcium phosphate) may be disadvantageous, as dissolution behaviors are not always as long-lasting as required, and degradation products may not be completely cytocompatible [5,6]. The non-controllable calcium release of these materials may lead to inhibition, stimulation or no effect on osteoconductivity, osteoclastic activity or even fibrous formation at the implanted tissue.…”

Section: Introductionmentioning

confidence: 99%

“…The non-controllable calcium release of these materials may lead to inhibition, stimulation or no effect on osteoconductivity, osteoclastic activity or even fibrous formation at the implanted tissue. The attained effect will depend on the variations of extracellular calcium concentration values [5,6], and it is not predictable with reabsorbable materials.…”

Section: Introductionmentioning

confidence: 99%

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Bioactive Polymeric Nanoparticles for Periodontal Therapy

et al. 2016

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Aimsto design calcium and zinc-loaded bioactive and cytocompatible nanoparticles for the treatment of periodontal disease.MethodsPolymP-nActive nanoparticles were zinc or calcium loaded. Biomimetic calcium phosphate precipitation on polymeric particles was assessed after 7 days immersion in simulated body fluid, by scanning electron microscopy attached to an energy dispersive analysis system. Amorphous mineral deposition was probed by X-ray diffraction. Cell viability analysis was performed using oral mucosa fibroblasts by: 1) quantifying the liberated deoxyribonucleic acid from dead cells, 2) detecting the amount of lactate dehydrogenase enzyme released by cells with damaged membranes, and 3) by examining the cytoplasmic esterase function and cell membranes integrity with a fluorescence-based method using the Live/Dead commercial kit. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests.ResultsPrecipitation of calcium and phosphate on the nanoparticles surfaces was observed in calcium-loaded nanoparticles. Non-loaded nanoparticles were found to be non-toxic in all the assays, calcium and zinc-loaded particles presented a dose dependent but very low cytotoxic effect.ConclusionsThe ability of calcium-loaded nanoparticles to promote precipitation of calcium phosphate deposits, together with their observed non-toxicity may offer new strategies for periodontal disease treatment.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioactive Polymeric Nanoparticles for Periodontal Therapy

et al. 2016

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“…Cuando analizamos otros modelos experimentales de reconstrucción ósea en animales, encontramos un número reducido de modelos de resección en bloque y éstos se basan, principalmente, en cirugía de huesos largos y en animales de mediano tamaño como perros, caprinos, porcinos o primates (Yamauchi et al, 2010;Masaoka et al;Tomco et al, 2017). Estos modelos en animales son útiles ya que debido a su tamaño, el procedimiento en general (cirugía, bloques y elementos de osteosíntesis) es más cercano a la realidad de lo que ocurre en humanos, sin embargo por el mismo tamaño de los animales su manejo es más complejo que la utilización de animales de pequeño porte como son los conejos; en este sentido, el análisis bioético es fundamental y parte central al diseñar y justificar un estudio experimental en animales (Hart;Ormandy & Griffin, 2016 Tabla II.…”

Section: Discussionunclassified

Modelo Experimental para el Estudio de la Reconstrucción Mandibular: Opciones en Conejos Oryctolagus cuniculus

et al. 2017

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Morphol., 35(3):1185Morphol., 35(3): -1190Morphol., 35(3): , 2017. RESUMEN:El propósito de este estudio fue de evaluar la técnica quirúrgica propuesta para estudios de resección y reconstrucción mandibular en modelos experimentales en conejos. Se utilizaron 7 conejos neozelandeses (Orictolagus cuniculus), en los cuales se realizó la resección del cuerpo mandibular izquierdo, una vez asegurada la movilidad de los segmentos, se reconstruyó con placa de osteosíntesis de titanio de 1,5 mm y tornillos monocorticales de 5 mm, en la zona resecada se fijó un bloque de beta fosfato tricálcico y estabilizado con tornbillo de 1,2 mm. Se sacrificaron los animales a los 2 meses. Todos los animales sobrevivieron al procedimiento y se pudo llevar a cabo la resección y reconstrucción en todos ellos. No se presentaron signos ni síntomas de infección en el sitio quirúrgico mientras duró el experimento. El tiempo promedio de cirugía fue de 68 minutos. El peso promedio de los animales fue de 3925 g, la pérdida promedio de peso fue de 2,03 %. No se realizó eutanasia de ningún animal por motivos de sufrimiento. Al examen macroscópico de las muestras extraídas se observó la presencia de todos los elementos de osteosíntesis en posición, así como continuidad de la estructura ósea casi en su totalidad en la superficie . La utilización de conejos en modelos de reconstrucción maxilofacial es un modelo probado debido a la similitud de los procesos reparativos, su facilidad de manipulación y cuidados. El modelo propuesto representa una alternativa más cercana a los procesos reconstructivos en cirugía resectiva maxilofacial, debido a que somete los injertos a las fuerzas propias de la masticación y de la función orofacial.PALABRAS CLAVE: Injerto óseo; Scaffold; Ingeniería de tejidos; β β β β β-TCP.

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“…In this study we focused on augmentation of flat bone area. Study of bone augmentation using periosteal distraction device 25) or titanium screws 17) have already been reported in animal experiments. Shape stability of bone grafting is easy to obtain by using a surgical plate or surgical mesh in clinical case.…”

Section: Discussionmentioning

confidence: 99%

“…Hydroxyapatite (HA) granules [1][2][3] andtricalcium phosphate paste (P ) [4][5][6] are widely used as bone substitutes in patients with fractures, bone defects or absorption of the alveolar ridge 7,8) . On the other hand, animal studies have led to the developments of new techniques, including autogenous bone onlay grafting [9][10][11][12] and artificial bone block 3,6,[13][14][15][16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

Augmentation of Flat Bone Area Using Tetrapod-Shaped Artificial Bone in Rats

Iwata

Namaki

Mashimo

et al. 2015

J. Hard Tissue Biology.

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The aim of this study was to compare bone augmentation height and mechanical strength after implantation of tetrapod-shaped granular artificial bone (Tetrabone® [TB]), hydroxyapatite granules [HA], -tricalcium phosphate paste [P ] or autogenous onlay bone grafts (veneer graft [VN]). Forty-five 6-week-old male Wistar rats were used. The graft materials were implanted in the left tibial subperiosteal pocket. Bone augmentation was evaluated by computed tomography (CT), mechanical testing and histological observation immediately after surgery (0 weeks) and 4, 8, and 12 weeks later. The augmentation height was measured in CT images and the proportion of bone augmentation was calculated as the ratio of 12 weeks/0 weeks. TB aggregates were smooth in shape compared with HA aggregates. The augmentation height was significantly higher in the TB group than in the VN group. At 12 weeks, bone stiffness was significantly higher in the TB group than in the HA and P groups, and very similar to that in the VN group. TB presented good shape stability and mechanical strength. TB might be useful for bone augmentation of flat bone area as compared with other currently used artificial graft materials. Future studies should focus on the clinical application of TB grafts.

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Histological and histomorphometrical comparative study of β-tricalcium phosphate block grafts and periosteal expansion osteogenesis for alveolar bone augmentation

Cited by 38 publications

References 27 publications

Bioactive Polymeric Nanoparticles for Periodontal Therapy

Bioactive Polymeric Nanoparticles for Periodontal Therapy

Modelo Experimental para el Estudio de la Reconstrucción Mandibular: Opciones en Conejos Oryctolagus cuniculus

Augmentation of Flat Bone Area Using Tetrapod-Shaped Artificial Bone in Rats

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