Histological changes and biochemical parameters in the hepatopancreas of terrestrial gastropod Helix aspersa as biomarkers of neonicotinoid insecticide exposure

Abstract

In this study, adult snails, Helix aspersa were used to estimate the effect of aneonicotinoid insecticide (thiametoxam) on biochemical parameters and histological changes in the hepatopancreas of this gastropod after a treatment of six weeks. During this period, snails were exposed by ingestion and contact to fresh lettuce leaves which were soaked with an insecticide solution. The thiametoxam test solutions were 0, 25, 50, 100 and 200 mg/L. The results of the biochemical dosages (total carbohydrates, total proteins and total lipids) showed significant decreases at two concentrations (100 and 200 mg/L) of thiametoxam. However, the histological examination of the hepatopancreas of the treated snails showed alterations as a response to all the treatments, and revealed the degeneration of the digestive tubules and the breakdown of the basement membrane in a dose-dependent manner, leading to a severe deterioration of the tissues in the concentration of 200 mg/L thiametoxam. The dosage of carbohydrates, lipids and proteins supported by the study of histological changes on the hepatopancreas of H. aspersa can be considered as potential biomarkers of exposure to thiametoxam.

Toxicological assessments of basic blue 3 dye in fresh water bivalve Lamellidens marginalis

“…After treatment to dyes for different concentration and exposure period showed the degeneration of basement membrane, swelling and degeneration of digestive tubules, fragmentation of digestive cells was observed. These results are similar to the work done by [21,43,23,22] . The digestive gland of bivalve Crenomytilusgrayanus showed vacuolization of digestive cells, lipofuscin, necrosis and lysis of cells in connective tissue due to polluted water [53] .…”

“…The digestive gland of bivalve Crenomytilusgrayanus showed vacuolization of digestive cells, lipofuscin, necrosis and lysis of cells in connective tissue due to polluted water [53] . The hepatopancreas of Helix aspera showed the damages to the digestive tubules, breakdown of the basement membrane leading to severe damages like deterioration of the tissue after introduction of Thiamethoxam [22] . The gonad showed different developmental stages viz.…”

Physiological responses of oxidative damage, genotoxicity and hematological parameters of the toxic effect of neonicotinoid-thiamethoxam in Oreochromis niloticus

“…Antioxidant enzymes inhibited the activity of detoxi cation by excessive ROS, which disrupts the detoxi cation tasks and damages the cellular function of the living organism and leads to DNA damage (Kim and Hyun 2006). AChE enzyme activity is not a detoxifying enzyme in vertebrates but is involved in the transmission of nervous system transmission in every tissue of the living organism through neurotransmission mechanisms (Smina et al 2016). Acetylcholinesterase AChE is a cholinergic enzyme that regulates enzyme activity by hydrolyzing the neurotransmitter acetylcholine (ACh) in nerve cells (Sera ni et al 2019).…”

Human health risk assessment on the consumption of Musa acuminata treated with different ripening agents

“…9, 2021: ISSN 2449-0814 Nwankwo et al 37 health concerns because potentially toxic elements bioaccumulate in the systems of most organisms and biomagnify over time (Orisakwe et al, 2012), disrupting various biological processes in the body. The ability of the human defense mechanism, such as antioxidants, to combat these toxic element exposures may deteriorate with repeated exposure, stimulating a disease condition or exacerbating some health risk condition (Hamlet et al, 2012).…”