Histological Changes and Testicular Dysfunction in Severely Burned Rats

Jewo, P.I.; Duru, Francis Ikechukwu; Osinubi, AA; Fadeyibi, IO; Faduyile, Francis Adedayo; Benebo, A. S.

doi:10.3889/mjms.1857-5773.2011.0171

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…Post-burn infections are a major cause of mortality due to severe types of infections. The compromised immune system of burnt patients makes them susceptible to microbial infections by bacterial or fungal pathogens [15,25]. Here, burnt patients were observed with a mean age of 28 ± 15.65 years while Melake et al, reported 15 ± 17.60 years mean age [4].…”

Section: Discussionmentioning

confidence: 99%

Risk factors analysis: screening of extended-spectrum-β-lactamase producing Gram-negative isolates of burn infections from tertiary care hospital Lahore, Pakistan

Haider

Ain

Hussain

et al. 2019

Preprint

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Background Burnt patients are highly susceptible to invasion of multidrug resistant strains after the skin damage. The main objective of this study was to estimate the frequency of ESBLs producing Gram-negative in post-burn infections and its correlation with different risk factors (age, gender, socio-economic status, burns etiology etc.) Methods The descriptive case-series study was conducted at Jinnah Hospital Lahore’s Burn and Reconstructive Surgery Centre (JB & RSC) and the Department of Microbiology and Molecular Genetics (MMG), University of the Punjab, Lahore. The clinical specimens of wound swabs, biopsy, and blood were collected from 300 patients during 12th August, 2017 to 12th August 2018. The cephalosporins resistant Gram-negative isolates were further analyzed. The clinical history of burnt patients was recorded which included the age, gender, socio-economic status, residence, occupation, hospital stay, wards, burn degree, total burnt surface area (TBSA%), etiology of burn and other factors. CLSI guidelines 2017 were followed for the antimicrobial susceptibility testing (AST) and ESBLs screening. Results Pseudomonas spp. were the most-frequently isolated 49.33% (n=74) followed by 22.67% (n=34) Klebsiella spp., and 20.00% (n=30) Acinetobacter spp., strains. Pseudomonas spp., were the most frequently isolated from burnt specimens 70 (46.67%) having a significant correlation (x2=24.11, p < 0.001). Community acquired infections were observed in 50.70% (n=76) and nosocomial infections in 49.30% (n=74) patients. Burnt people having the age of ≤ 29 years were found to be significantly associated with the MDR infections (x2=24.96, p = 0.003). Low socio-economic status, longer hospitalization and all other risk factors had non-significant correlation (p > 0.05). A large fraction of the isolates 86.00% (n=129) were not confirmed as ESBLs producers by phenotypic screening. Conclusion It has been concluded that Gram-negative MDR strains are rapidly causing infections in burnt patients and need to be properly alleviated. The high frequency of multidrug resistant Pseudomonas spp., was associated with the burn infections. The patients belonging to young age were significantly found to be infected by MDR strain after burns.

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Section: Discussionmentioning

confidence: 99%

Risk factors analysis: screening of extended-spectrum-β-lactamase producing Gram-negative isolates of burn infections from tertiary care hospital Lahore, Pakistan

Haider

Ain

Hussain

et al. 2019

Preprint

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“…The back and flank skin of the rats were shaved. Rats were placed in supine position in a plaster cast with an area of their backs exposed through an opening in the cast, immersed in a hot water bath (100°c) for 10 seconds (Jewo et al, 2011). This should produce non lethal full thickness injury to the skin that covers 20 % of the total body surface area which were calculated by using the formula of Lee which is: Total body surface area (TBSA) = (body weight in grams x 0.78) + 148 (Farahat, 1999).…”

Section: -Experimental Designmentioning

confidence: 99%

“… Group II (sham group): Rats were anesthetized and given analgesic (with an intraperitoneal injection of ketamine hydrochloride 20 mg/ kbw and diazepam 0.1 mg/kbw) and shaved, then put belly up in tap water for 10 seconds and removed.  Group III (scald injured group):Rats were anesthetized and given analgesic (with an intraperitoneal injection of ketamine hydrochloride 20 mg/kbw and diazepam 0.1 mg/kbw) and shaved, then immersed into hot water bath (100°c) for 10 seconds to produce full thickness injury to the skin (Jewo et al, 2011). At the end of the specified durations (6, 12 and 24 hours), six rats for each time point from each group were anesthetized and subjected to blood sampling for estimation of AST, ALT and ALKP levels, then they were sacrificed, the liver and brain were subjected to histopathological Mostafa et al, / Ain Shams J Forensic Med Clin Toxicol, July 2014 (23):12-20 examination using H & E stained sections and immunohistochemical staining for TNF-α activity.…”

Section: Grouping Of Animalsmentioning

confidence: 99%

Effects of Scald Injuries on Liver and Brain Adult Male Albino Rats (Biochemical, Histopathological and Immunohistochemical Study)

Mostafa

El-Din

Hassan

2014

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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Thermal injuries still occur frequently in many parts of the world. Severe cutaneous thermal injuries are associated with multiple organ dysfunctions, which demand urgent medical interventions to ensure patient's survival. This failure of different organ systems can be attributed to uncontrolled production of inflammatory cytokines such as interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), interleukin 1beta (IL-1β). The purpose of this study was to detect the effects of scald injuries on liver and brain of adult male albino rats at 6, 12 and 24 hours post injury. Material and methods: Fifty four adult male albino rats were equally divided into three groups: group I (negative control group), group II (sham group) and group III (scald injured group) effects of scald injuries on liver and brain at 6, 12 and 24 hours post injury were studied through: estimation of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and Alkaline phosphatase (ALKP) levels and histopathological examination using light microscope including HandE stained sections and immunohistochemical staining of sections for detection of (TNF-α) activity.Results: In group III (scald injured group), a markedly affected and disturbed liver functions represented by significant increases in serum AST, ALT and ALKP levels, histopathological changes were (aggregations of inflammatory cells, congestion and dilatation of blood vessels, vaculated hepatocytes with darkly stained nucleui and hydropic degeneration) and (shrunken neurons with pyknotic nuclei, vacuolated cytoplasm and edema) .Time dependent TNF-α activation was detected in liver and brain at 6 h post injury lasting till 24h.Conclusion: From the previous results it can be concluded that, scald injury induced a marked affection and disturbance in liver functions, as characterized by significant increases in serum ALT, ASTand ALKP, histopathological changes in liver and brain. Also, a time dependent TNF-α activation was detected in liver and brain of adult male albino rats.

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Liver Dysfunction in Sternness Burn Injury in Association to Age Estimate: A Conceivable Role of Antioxidant Biomarker and Tumor Necrosis Factor Alpha

Ibrahim¹,

Kandeel²

2022

JBM

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Histological Changes and Testicular Dysfunction in Severely Burned Rats

Cited by 3 publications

References 22 publications

Risk factors analysis: screening of extended-spectrum-β-lactamase producing Gram-negative isolates of burn infections from tertiary care hospital Lahore, Pakistan

Risk factors analysis: screening of extended-spectrum-β-lactamase producing Gram-negative isolates of burn infections from tertiary care hospital Lahore, Pakistan

Effects of Scald Injuries on Liver and Brain Adult Male Albino Rats (Biochemical, Histopathological and Immunohistochemical Study)

Liver Dysfunction in Sternness Burn Injury in Association to Age Estimate: A Conceivable Role of Antioxidant Biomarker and Tumor Necrosis Factor Alpha

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