Histological Changes in Human Gallbladder in Pathological Condition Including Cholecystitis and Cholelithiasis an Analytical Study

Pani, JyotiPrakash

doi:10.9790/3013-034301-13

Cited by 3 publications

(3 citation statements)

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“…Pani et al found that a major amount of mucin in cholelithiasis and cholecystitis was sulfomucin and sialomucin, respectively. Similar to intestinal epithelium, the gallbladder also showed increased mucin secretion for stone formation and acceleration which leads to concomitant exhaustion of mucin content within the lining epithelium [ 14 , 15 ]. Finally, in this study, we found that inflammation causes mucin alteration, which is exactly opposite to normal gallbladder mucin.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Mucin Expression Using Combined Alcian Blue-Periodic Acid Schiff (AB-PAS) Stain and Combined High Iron Diamine-Alcian Blue (HID-AB) Stain and the Correlation With Histomorphological Score in Chronic Calculous Cholecystitis

Vadivazhagan¹,

Kalaivani²,

Sudalaimuthu³

2022

Cureus

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IntroductionChronic calculous cholecystitis is the most common and important contributing factor for cholecystectomy across the country, with a prevalence of 2-29%. Cholesterol supersaturated bile plays a major role in stone formation. It is very essential to identify the pathogenesis of stone formation in order to prevent its formation. This study is aimed to evaluate histomorphological features of chronic calculous cholecystitis and to quantitatively evaluate alteration in mucin expression using the combined Alcian blue-periodic acid Schiff (AB-PAS) stain and the combined high iron diamine Alcian blue (HID-AB) stain, to correlate with each other and also with biochemical features of gall stones. MethodsA cross-sectional study of 64 chronic calculous cholecystitis were taken for histomorphological assessment and grading using Hematoxylin and Eosin (H&E) stain and Masson's trichrome stains. Expression of the type of mucins was analyzed using histochemical stains by a standardized scoring system. ResultsA significant positive correlation was observed between an increase in grades of inflammation and fibrosis with an increase in the quantity of sialomucin and neutral mucin in the deep layers of epithelium, and a significant negative correlation was observed between an increase in grades with a decrease in acidic mucin and sulfomucin of both superficial and deep epithelium except sulfomucin in fibrosis. No significant correlation was obtained with muscle thickness, adipose tissue deposition, and epithelial hyperplasia. A higher frequency of mixed-type stones was associated with severe inflammation. ConclusionInflammation and fibrosis were strongly correlated with quantitative alteration and reversal of mucin composition in chronic cholecystitis; hence we conclude that these features play a significant role in the pathogenesis of stone formation. Using Combined AB(2.5pH)-PAS stain and Combined HID-AB(2.5 pH) stain to detect mucin hypersecretion and composition of altered mucin is relatively accurate and cost-effective rather than performing costly immunohistochemical (IHC) markers.

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Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Mucin Expression Using Combined Alcian Blue-Periodic Acid Schiff (AB-PAS) Stain and Combined High Iron Diamine-Alcian Blue (HID-AB) Stain and the Correlation With Histomorphological Score in Chronic Calculous Cholecystitis

Vadivazhagan¹,

Kalaivani²,

Sudalaimuthu³

2022

Cureus

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“…Due to the enhanced intake of wealthy calories and fatty diet and increasing alcoholic consumption, the prevalence of cholecystitis and colelithiasis has risen globally over the previous couple of centuries. 2,3 The rate of gallstone disease differs depending on era, gender, ethnic groups and regions. In Americans and Indians, the incidence of gall liver in women is strong at 60% -70%; Chile (is a nation of South America) at 37%; the UK and the US at 6% to 12% and 11%, respectively, at 6 The incidence frequency of China and Japan is very small, 5%.…”

Section: Introductionmentioning

confidence: 99%

Study on morphological changes in the gall bladder mucosa associated with cholelithiasis.

Bano

Bakhsh

Wagan

et al. 2020

TPMJ

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Cholelithiasis is known to produce diverse histopathological changes in the gallbladder mucosa. Objectives: To observe the morphological changes in the gall bladder mucosa associated with cholelithiasis. Study Design: Descriptive Cross Sectional study. Setting: Department of Anatomy, Jamshoro with collaboration of Department of Pathology, LUMHS, Jamshoro. Period: April 2016 to October 2016. Material and Methods: All the cases after diagnosis of cholelithiasis with all age groups (20 to 50 years) in male and female were included. Gallstones were assessed for various parameters, i.e., number, size, and morphological type. Gallbladder mucosa was subjected to histopathological examination. Sections of the body, the fundus and the bottom of the gallbladder were drawn. 01 cm of the body, the body, and the bottom of the gallbladder were drawn from the funduse and prepared for histological research. Data was statistically analyzed by SPSS (Statistical Package of Social Sciences) version 22.0. Results: Total 87 cases were selected and average age of 49.95±6.14 years of male was seen in a gall bladder width and length in the gallbladder and gallbladder length in both sex of P-value 0.07 while male of female average were 41.30añ6.62, age range from 30 to 52 years. Sixty-seven (77.0%) cases had multiple stones. 29 (33.3 percent) patients had congested the serological layer, while 58 (66.7 percent) were usually impacted. Mucosa hemorrhagic in 22(25.3%) cases, atrophic in 51(58.6%) cases and nodular in 4(4.6%) cases. 36(41.4%) gall bladders reported normal results, 41(47.1%), 1(1.1%), and 3(3.4%) were identified with Fibrosis, and 2(2.3%) are found with Fibrosis. Many cases were linked to fibrosis, dysplasia, and hyperplasia, compared to single calculus (P value 0.048). There was no substantial difference between normal and thickened gall bladders in the microscopic tests (P value 0.26). Fibrosis, dysprasia and hyperplasia are correlated mainly with internal mucus detections (P value 0.002). Hemorrhagic mucous membranes are found mainly. Conclusion: Hemorrhagic and fibrotic changes were the most common mucosal findings, and significantly associated with multiple cholelithiasis.

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“…2 The incidence of cholecystitis and choleilithiasis has increased over the past couple of decades throughout the world and is attributed to the increased intake of fatty, high calorie diet and increased consumption of alcohol. 3,4 The prevalence of gall stone disease varies with age, sex, ethnic groups and geographical areas. The female prevalence of gall bladder disease in the American Indians and Pima Indians is as high as 60%-70%, Chile 37% while in UK and in the USA it is 6% to12% and 11% respectively.…”

Section: Introductionmentioning

confidence: 99%

Morphological spectrum of gall bladder lesions and their correlation with cholelithiasis

Dattal¹,

Kaushik²,

Gulati³

et al. 2017

Int J Res Med Sci

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Background: Gall stones are known to produce diversified histological changes in the gallbladder such as inflammation, metaplasia, hyperplasia, dysplasia and even precursor lesions of malignancy. The aim of our study was to correlate the various histological changes in the gallbladder with the different types of gallstones (cholesterol, pigmented and mixed).Methods: This prospective study was conducted in the department of Pathology, Indira Gandhi Medical College, Shimla, Himachal Pradesh from July 2014 to June 2015. The age, sex distribution and the incidence of different types of gallstones was studied. The histological changes in the gallbladders were examined and its correlation with the type of gallstones was evaluated.Results: In all 1371 specimens of gall bladder were received during this period. The mean age of the patients was 44 years (age range 13-86 years) with Male: Female ratio of 1: 8.3. Out of the total 1371 cholecystectomy specimens, 1259 (91.8 %) had calculi and 112 (8.2%) were acalculus. Of the 1259 cases of calculus disease, mixed stones were observed in 840 (66.7%) patients, cholesterol stones in 289 (22.9%) and pigmented stones in 130 (10.4%) patients. Overall 1291 (94%) cases had chronic cholecystitis followed by acute cholecystitis and chronic active cholecystitis in 2% each, dysplasia in 1.5% and carcinoma cases 0.5%.Conclusions: Routine cholecystectomy performed for a common condition like gallstone disease can result in detection of diverse and wide spectrum of histopathological lesions ranging from chronic cholecystitis to carcinoma.

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Histological Changes in Human Gallbladder in Pathological Condition Including Cholecystitis and Cholelithiasis an Analytical Study

Abstract: : Present study compares histological changes in the wall

Cited by 3 publications

References 12 publications

Quantitative Analysis of Mucin Expression Using Combined Alcian Blue-Periodic Acid Schiff (AB-PAS) Stain and Combined High Iron Diamine-Alcian Blue (HID-AB) Stain and the Correlation With Histomorphological Score in Chronic Calculous Cholecystitis

Quantitative Analysis of Mucin Expression Using Combined Alcian Blue-Periodic Acid Schiff (AB-PAS) Stain and Combined High Iron Diamine-Alcian Blue (HID-AB) Stain and the Correlation With Histomorphological Score in Chronic Calculous Cholecystitis

Study on morphological changes in the gall bladder mucosa associated with cholelithiasis.

Morphological spectrum of gall bladder lesions and their correlation with cholelithiasis

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