Histological Changes in the Small Intestine in the Advanced Stages of Burn Disease

Galunko, G. M.

doi:10.26724/2079-8334-2017-3-61-90-96

Cited by 6 publications

(11 citation statements)

References 6 publications

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“…Histological studies have shown that changes in the structural components of the thyroid gland follicles during the experimental thermal trauma develop in the background of microcirculation disturbance, which is to some extent consistent with the studies [1,7,11,21]. Significantly disturbed ultrastructure of the walls of perifollicular blood capillaries.…”

Section: Discussionsupporting

confidence: 84%

“…It has been established that at separate sites in the follicles, the thyrocytes do not adhere tightly to the wall of the (6), branching of the basal surface of the thyrocytes (7). x12000.…”

Section: Resultsmentioning

confidence: 99%

“…Burns of the third degree were applied under ketamine anesthesia with copper plates heated in boiled water to a temperature of 97-100°C [7,22]. The size of the lesions was 18-20% of the shaved surface of the body of rats.…”

Section: Methodsmentioning

confidence: 99%

“…In the case of severe thermal trauma, there are a number of factors that lead to sepsis and multiple organ failure: microcirculation disorder, presence of necrotic tissues inhabited by wounds, endogenous intoxication, activation of lipid peroxidation, development of syndrome of systemic inflammatory response, DIC, etc. [2,3,5,6,7,10,13,14,20]. It has been established that in the complicated and inadequately studied pathogenesis of burn disease one of the main places has endogenous intoxication, which is the result of proteolysis of damaged tissues and alteration of histo-hematological barriers.…”

Section: Introductionmentioning

confidence: 99%

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Features of the thyroid gland structural components remodeling in the toxemia stage after experimental thermal injury

Koritskiy

Небесна

2018

Rep. of Morph.

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Thermal injury causes severe structural and metabolic disturbances not only of the skin itself, but also of all organs and systems of the affected organism, is a manifestation of a complex symptom complex - a burn disease. Of particular importance in patients with burns are changes in the endocrine system. The aim of the study was to establish the microscopic and electron-microscopic reorganization of the components of the thyroid gland of animals after thermal damage on 14 day after experimental thermal injury. A III degree burn was applied under ketamine anesthesia with copper plates heated in boiled water to a temperature of 97-100°C. The size of the lesion area was 18-20% of the epilated surface of the body of rats. An experimental study of the structural components of the thyroid gland after a burn injury was performed on laboratory white male rats weighing 160-180 g. Rats euthanasia was performed after ketamine anesthesia by decapitation. In the experiment, the study of the microscopic and submicroscopic state of the follicles and hemocapillaries of the thyroid gland after thermal injury of the III degree. It has been established that in the toxemia stage after the application of the burn injury on 14 day (late toxemia stage), significant destructive and degenerative changes are found in the thyrocytes of the wall of the follicles and hemocapillaries, the organ acquires a macrofollicular structure. The height of thyrocytes decreases, the nuclei and organelles of cytoplasm are significantly damaged, the number and height of microvilli on their apical surface decreases, which negatively affects the cell's secretory cycle and transcapillary organ metabolic processes. The established destructive changes in the blood capillaries and thyrocytes of the follicles are the morphological manifestation of the suppression of the secretory activity of the thyroid gland during thermal injury and corresponds to the hypofunctional state of the organ.

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Section: Discussionsupporting

confidence: 84%

“…It has been established that at separate sites in the follicles, the thyrocytes do not adhere tightly to the wall of the (6), branching of the basal surface of the thyrocytes (7). x12000.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Features of the thyroid gland structural components remodeling in the toxemia stage after experimental thermal injury

Koritskiy

Небесна

2018

Rep. of Morph.

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“…Опікова травма є однією з широко розповсюджених травматичних пошкоджень і спостерігається у 5,6-12,0% від усіх потерпілих з іншими видами травматизму [3,4,6,11,14,18]. У відповідь на опікову травму в організмі розвивається велика кількість патологічних процесів, які охоплюють практично усі органи і системи, приводячи до вираженого порушення гомеостазу, зриву адаптаційних процесів [2,4,10,11,12,13,15,19]. На даний час зростає інтерес до вивчення механізмів пошкодження і структурної перебудови органів ендокринної системи, викликаної різними патологічними факторами.…”

Section: вступunclassified

Peculiarities of structural reorganization of the thyroid gland vessels in dynamics after experimental thermal trauma

Korytskyi¹

2018

Rep. of Vinnytsia Nation. Med. Univ.

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Thermal trauma and burn disease, which develops from deep, large lesions, are accompanied by significant morphological and functional changes not only in the skin, but also in organs of all systems of the body, including thyroid gland. The purpose of the study was to establish a microscopic reorganization of the animal thyroid gland in dynamics after an experimental thermal trauma. Burns of the third degree were applied under ketamine anesthesia with copper plates heated in boiled water to a temperature of 97–100 °С. The affected area corresponded 18–20% of the surface of the rat’s body. Experimental study of structural changes in the vascular bed of the thyroid gland after severe thermal trauma was carried out on laboratory white male rats weighing 160–180 g. Euthanasia of rats was performed after ketamine anesthesia, by decapitation. Structural changes in the thyroid gland were studied after 1, 7, 14 and 21 days from the beginning of the experiment. Histologic sections of 5–6 µm thickness were stained with hematoxylin-eosin. For the study of the connective tissue condition and its main substance, silver impregnation was performed by using the Gordon-Sweet's method (to detect reticular and immature collagen fibers), staining with 3-colored MSB — selective coloring method for intravascular coagulation syndrome. It has been established that in the early stages after the thermal trauma (1–7 days of the experiment, the stage of shock and early toxemia) there are adaptive-compensatory changes and initial signs of destruction of the vascular bed structural components of the organ. In late terms (14–21 days of the experiment, the stages of late toxemia and septicotoxemia) significant destructive-degenerative changes of the thyroid gland arteries, veins and blood vessels of the hemocirculatory bed were detected. Therefore, a severe burn injury causes deep histological modification of the thyroid gland vessels, the degree of intensity of which depends on the time interval of the experiment, which adversely affects on the functioning of the organ and transvascular metabolic processes. In further researches it is planned to study the course of morphological changes of thyroid gland vessels during thermal trauma with combined application of corrective methods.

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Microscopic changes of the pancreas vessels in the dynamics after experimental thermal injury

Zykova

2021

Biomed. and Biosoc. Anthrop.

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The problem of burns is relevant in the world and in Ukraine. Thermal injury is not limited to local tissue changes, but causes significant morphofunctional disorders in the organs and systems of the affected organism, the result of this factor is a violation of blood supply to organs, including the pancreas. The aim of this work was to establish the histological condition of the vessels of the pancreas after experimental thermal injury. The experiments were performed on 40 adult white rats. Burns were applied under thiopental-sodium anesthesia with copper plates at a temperature of 96-97℃ on the epilated surface of the skin of the back of animals for 15 seconds (the lesion area was 18-20 % of the body surface). Decapitation of animals was performed on 1, 7, 14 and 21 days of the experiment. Pieces of the pancreas were taken for histological examination and processed according to generally accepted methods. Histological sections 5-6 μm thick were stained with hematoxylin-eosin, impregnated with silver by the Gordon-Sweets method, as well as staining by the tricolor method of MSB (ORB). Histological specimens were examined and documented using an SEO SCAN light microscope and a Vision CCD Camera. In the early stages of the experiment revealed a violation of the blood supply to the body. The lumens of most arteries were found to be dilated, and the wall thickness increased due to hypertrophy of the smooth muscle cells of the media. Leukocyte infiltration was determined in the perivascular spaces. The lumens of the small and middle veins were dilated and filled with blood, with parietal thrombi in some of them. Capillaries are characterized by a moderate expansion of their lumens, stasis. There was swelling of the paravasal spaces. Significant destructive-degenerative, inflammatory and sclerotic changes were observed in the later stages of the experiment. The walls of the vessels were moderately swollen. Smooth myocytes of the arterial media were hypertrophied, the cytoplasm was vacuolated, and contained pyknotically altered nuclei. Adventitia of vessels, especially veins, was swollen and fibrous. Occasionally there were violations of the wall of the components of the microcirculatory tract, which was accompanied by hemorrhage. There are single thrombosed vessels, spasmed arterioles, hemocapillaries with dilated lumens. The greatest changes, especially on the 21st day, were the adventitial membrane with the growth of collagen and reticular fibers. The blood supply to the vessels was moderate. Vessels of small diameter and hemomicrocirculatory tract were also significantly altered, their wall is deformed, thin, homogeneous. The lumens are dilated, with the formation of blood clots. Thus, in the early stages of the experiment there are adaptive-compensatory processes and initial destructive changes in the walls of the vessels of the organ, manifested by stasis, thrombosis, edema, deformation, infiltration of the wall and perivascular space. In the late period, significant destructive-degenerative changes in the structure of the walls of the vessels of the pancreas, accompanied by hemorrhage, inflammation, sclerosis of the wall and edema of the adventitia. These changes lead to a deterioration of blood supply and, consequently, a decrease in the functional activity of the body.

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Histological Changes in the Small Intestine in the Advanced Stages of Burn Disease

Cited by 6 publications

References 6 publications

Features of the thyroid gland structural components remodeling in the toxemia stage after experimental thermal injury

Features of the thyroid gland structural components remodeling in the toxemia stage after experimental thermal injury

Peculiarities of structural reorganization of the thyroid gland vessels in dynamics after experimental thermal trauma

Microscopic changes of the pancreas vessels in the dynamics after experimental thermal injury

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