Histological changes in the vulva and vagina from ovariectomised rats undergoing oestrogen treatment

Mocan-Hognogi, Radu Florin; Costin, Nicolae; Măluţan, Andrei Mihai; Ciortea, Răzvan; Trif, I; Nagy, András; Bogdan, M L; Mihu, Dan

doi:10.5603/fm.a2016.0026

Cited by 4 publications

(6 citation statements)

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“…The histological analysis in our study revealed that the structural properties of the vaginal wall, including the epithelium, lamina propria, muscularis, and adventitia layers, are similar between rats and humans, thus providing a foundation for observing the impact of menopause on the vaginal wall. Mocan-Hognogi et al proved that surgically induced anestrus started 15 days after bilateral ovariectomy by detecting significantly decreased estradiol levels after surgery [19]. We also found that the epithelium began to atrophy 2 weeks after ovariectomy, which is consistent with previous studies [24, 25].…”

Section: Discussionsupporting

confidence: 92%

“…Therefore, we conducted a comprehensive analysis of rat vaginal wall changes by combining histological, biochemical, and biomechanical analyses both short- and long-term after surgically inducing menopause. In this study, we used an experimental rat model of surgically induced menopause through bilateral ovariectomy to form permanent anestrus [17, 19]. A previous study found that age had a significant effect on the response of the vagina and its supportive tissues to ovariectomy such that ovariectomy significantly affected these tissues in young rats but not in middle-aged rats [23].…”

Section: Discussionmentioning

confidence: 99%

“…On the first day of the study and 2, 4, and 16 weeks after ovariectomy, 12 (Group 1), 6 (Group 2), 6 (Group 3), and 12 (Group 4) rats, respectively, were sacrificed under complete anesthesia for the anterior vaginal wall tissue collection. Our decision to sacrifice the rats at 2 weeks was based on a previous study that demonstrated that surgically induced anestrus starts 15 days after surgery based on the pre- and postsurgery estradiol levels [19].…”

Section: Methodsmentioning

confidence: 99%

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Tissue Composition and Biomechanical Property Changes in the Vaginal Wall of Ovariectomized Young Rats

Mao

Zhang

et al. 2019

BioMed Research International

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Ideal animal models are needed to reflect the changes in the biochemical and biomechanical properties of the vagina that occur in pelvic organ prolapse (POP). In this study, we aimed to demonstrate the short and long-term effect of menopause on the biochemical and biomechanical properties of rat anterior vaginas. Here, Sprague-Dawley rats were bilaterally ovariectomized to induce menopause. Rats without ovariectomy served as the normal control group (n=12). The histology changes and the expression of collagen I, III, and a-SMA were assessed to indicate the biochemical changes in the vagina 2 weeks, 4 weeks, and 16 weeks after ovariectomy (n=6 for 2 and 4 weeks, n=12 for 16 weeks). Uniaxial biomechanical testing was conducted in the control group and ovariectomized rats 16 weeks after ovariectomy. Compared with the control group, the ovariectomy group showed a significant increase in the expression of collagen I 2 weeks after ovariectomy, while collagen III showed a declining trend. Two weeks after ovariectomy, the smooth muscle bundles began to become disorganized, and the fraction of smooth muscle in the nonvascular muscularis of the proximal vagina was significantly decreased (P<0.001). However, there was no difference in the expression of a-SMA in the distal vagina. Compared with the control group, the ovariectomy group had stiffer vaginas with a declining trend in the ultimate load 16 weeks after ovariectomy. In conclusion, surgically induced menopause had a significant short- and long-term impact on tissue composition and biomechanical properties of the rat vagina, which may lead to increased susceptibility to POP development.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Tissue Composition and Biomechanical Property Changes in the Vaginal Wall of Ovariectomized Young Rats

Mao

Zhang

et al. 2019

BioMed Research International

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“…We were especially interested in the correlation between MCit and ER + carcinoma cells, as some studies have proved that estrogen activates mast cells in ovarian endometriosis and that human uterine mast cells express ERβ (19,20). Others have shown that administration of estrogens leads to eosinophilic and MCs infiltrations (21). MCs play a pro-tumorigenic role in human bladder cancer through stimulating ERβ and it has been demonstrated in a murine model of bladder cancer that a selective ERβ antagonist can inhibit mast cell-promoted tumor growth (9).…”

Section: Cells (Mcs) In Breast Carcinomas Stained For Anti-tryptase (mentioning

confidence: 99%

Mast Cells as an Indicator and Prognostic Marker in Molecular Subtypes of Breast Cancer

Carpenco

Ceauşu

Cîmpean

et al. 2019

In Vivo

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Background/Aim: Mast cells (MCs) represent the most controversial non-malignant element of the tumor microenvironment. Our aim was to study how MCs density and distribution (intratumoral-MCit versus peritumoral-MCpt) relate to tumor grade and molecular subtypes. Materials and Methods: MCs tryptase immunohistochemistry was performed on 80 cases of breast carcinomas. Results: For Luminal A tumors, a partial correlation was detected between MCit and progesterone receptor (PR) (p=0.005). Luminal B tumors showed a significant correlation between MCpt and age (p=0.009), estrogen receptor (ER) (p=0.017) and PR (p=0.035). MCit and MCpt were strongly interrelated in this subtype (p=0.002) and in triplenegative breast cancers (p=0.002). In HER2 subtype, MCpt tumors were significantly correlated with HER2 (p=0.044). In G2 tumors, MCpt correlated with ER (p=0.015) and PR (p=0.038) while in G3 tumors ER correlated with both MCit (p=0.009) and MCpt (p=0.000487) tumors. Conclusion: MCs dynamics are strongly influenced by hormone receptors and HER2 status. MCit increased in aggressive tumor types and is a worse prognostic factor.

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“…Entretanto, esse efeito não foi observado nas usuárias de COC, porém a morfologia vaginal não foi avaliada (ZALENSKAYA et al, 2018). Em animais, a maioria dos estudos diz respeito à avaliação do trofismo vaginal em modelos de menopausa cirúrgica, já que este quadro causa grave atrofia da mucosa vaginal (BENOIT et al, 2017;HENRIQUES et al, 2011;MOCAN-HOGNOGI et al, 2016). Assim, pouco se sabe a respeito da ação dos COC sobre a morfologia vaginal na idade reprodutiva.…”

Section: Discussionunclassified

Incidência Da Síndrome De Burnout Em Enfermeiros Da Unidade De Terapia Intensiva Em Um Hospital Público De Grande Porte Em Pernambuco

Neves¹,

Santos²,

Moura³

et al. 2020

Tecnologia E Inovação Para O Cuidar Em Enfermagem

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Histological changes in the vulva and vagina from ovariectomised rats undergoing oestrogen treatment

Abstract: Estradurin or Sintofolin. (Folia Morphol 2016; 74, 4: 467-473)

Cited by 4 publications

References 29 publications

Tissue Composition and Biomechanical Property Changes in the Vaginal Wall of Ovariectomized Young Rats

Tissue Composition and Biomechanical Property Changes in the Vaginal Wall of Ovariectomized Young Rats

Mast Cells as an Indicator and Prognostic Marker in Molecular Subtypes of Breast Cancer

Incidência Da Síndrome De Burnout Em Enfermeiros Da Unidade De Terapia Intensiva Em Um Hospital Público De Grande Porte Em Pernambuco

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