Histological Changes Occurring in the Contralateral Testes of Prepubertal Rats Subjected to Various Durations of Unilateral Spermatic Cord Torsion

Cosentino, Marco; Nishida, Masao; Rabinowitz, Ronald; Cockett, Abraham T.K.

doi:10.1016/s0022-5347(17)49278-0

Cited by 164 publications

(114 citation statements)

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“…Unsal et al 16 Good results were also obtained by Avlan et al 17 , whose study involved 720° torsion and ischemic time of 4h, administration of selenium, and histological evaluation based on Cosentino et al 9 .…”

Section: Methodsmentioning

confidence: 93%

“…Yurtçu et al 20 performed 720º torsion of spermatic cord for 6h and administered melatonin before detorsion. Histological evaluation, conducted according to Cosentino et al 9 , revealed a good level of protection.…”

Section: Methodsmentioning

confidence: 99%

“…Histological variables (Table 1) were evaluated according to Cosentino et al 9 . Each testis was graded from 0 to 4 for each variable, with higher grades indicating greater tissue damage in the cross-section examined.…”

Section: Methodsmentioning

confidence: 99%

“…The histological classification proposed by Cosentino et al 9 now widely adopted for evaluating testicular tissue injury secondary to testicular ischemia-reperfusion, was used in the present study.…”

Section: Methodsmentioning

confidence: 99%

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Propofol effects on the morphology of rat testes subjected to testicular ischemiareperfusion

et al. 2012

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PURPOSE:To evaluate the effects of propofol as an inhibitor of tissue injury in testicular ischemia-reperfusion in rats. METHODS:30 Wistar rats were assigned to one of three groups of 10 animals: G1, testicular exposure alone; G2 and G3: testicular ischemia caused by left spermatic cord torsion of 720º. In G3, propofol was administered intraperitoneally at 20 mg/kg/h 45 minutes after spermatic cord torsion. In G2 and G3, spermatic cords were detorsioned after 60 min. In all three groups, testes were subsequently repositioned in the scrotum. After 90 days, bilateral orchiectomy was performed for histological examination. RESULTS:No abnormalities in seminiferous tubules were found in G1. In G2, 86.6% of left testes exhibited abnormalities, in contrast with 67.8% for right testes. In G3, these proportions were 57.3% and 45.6%, respectively. A statistically significant difference was found between G2 and G3. CONCLUSION: Propofol reduced the tissue damage in rat testes subjected to ischemia-reperfusion caused by spermatic cord torsion. Key words: Propofol. Testis. Ischemia. Reperfusion. Rats. RESUMO OBJETIVO:Avaliar os efeitos do propofol como inibidor da lesão tecidual na isquemia-reperfusão testicular em ratos. MÉTODOS:Trinta ratos Wistar foram distribuídos em três grupos de 10 animais. G1: apenas exposição testicular. G2 e G3: isquemia testicular por torção do cordão espermático esquerdo a 720º. G3, 45 minutos após a torção do cordão espermático foi administrado propofol 20mg/Kg/hora via intraperitoneal. Após 60 minutos, nos grupos 2 e 3 foram desfeitas as torções dos cordões espermáticos e em seguida os testículos dos animais dos três grupos foram reposicionados no escroto. Após 90 dias foi realizada a orquiectomia bilateral para análise histológica. RESULTADOS:Nos túbulos seminíferos do grupo 1 não se encontrou anormalidades. Nos túbulos seminíferos do Grupo 2, as anormalidades foram 86,6% nos testículos esquerdos e 67,8% nos testículos direitos. Houve diferença estatisticamente significativa quando se compararam os testículos dos grupos 2 e 3. CONCLUSÃO:O propofol minimiza a lesão tecidual em testículos de ratos submetidos à isquemia-reperfusão na torção do cordão espermático.Descritores: Propofol. Testículos. Isquemia. Reperfusão. Ratos. Propofol effects on the morphology of rat testes subjected to testicular ischemia-reperfusion

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Section: Methodsmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Propofol effects on the morphology of rat testes subjected to testicular ischemiareperfusion

et al. 2012

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“…The histological parameters was scored according to Cosentino and colleagues's classification (Table 1). 21 …”

Section: Histological Examinationmentioning

confidence: 99%

Montelukast protects against testes ischemia/reperfusion injury in rats

Öztürk

Gideroğlu

et al. 2013

CUAJ

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Introduction: In this study, we investigate the effect of montelukast on histologic damage induced by testicular torsion-detorsion in rats. Methods: Twenty-one male Sprague-Dawley rats were separated into 3 groups, each containing 7 rats. A sham operation was performed in group 1 (control). In group 2 (ischemia-reperfusion [I-R]/untreated), 1-hour detorsion of the testis was performed after 6 hours of unilateral testicular torsion. In group 3 (I-R/dextroamphetamine), after performing the same surgical procedures as in group 2, montelukast was given intraperitoneally. In all experimental rats, ipsilateral orchiectomies were performed for histological examination and tissue malondialdehyde (MDA), glutathione and myeloperoxidase assays. Results: Montelukast treatment significantly decreased the I-Rinduced elevation in testes tissue MDA and glutathione levels were found to be preserved. The level of myeloperoxidase (MPO) activity was significantly increased in the testes tissue of the I-R/untreated group. However, in I-R/montelukast treatment group significantly decreased testes tissue MPO level. Histopathologically, the in the group 2 rats, edema, congestion, hemorrhage between seminiferous tubules and necrosis of the germinal cells were predominant features in sections. However, most of the specimens in the montelukast treated group 3 showed grades-I and II injury. Additionally, the testicular injury score was lower in group 3 rats compared with group 2. Conclusion: The current findings demonstrate that the montelukast decreased the severity of testicular injury by reversing the oxidative effects of testes I-R. RésuméIntroduction : Dans cette étude, nous examinons l'effet du montélukast sur les lésions tissulaires provoquées par torsion-détorsion testiculaire chez le rat. Méthodologie : Vingt-et-un rats Sprague-Dawley mâles ont été répartis en trois groupes de 7 rats. Une opération fictive a été réalisée dans le groupe 1 (groupe témoin). Dans le groupe 2 (ischémie-reperfusion / non traité), une détorsion testiculaire d'une heure a été réalisée après 6 heures de torsion testiculaire unilatérale. Dans le groupe 3 (ischémie-reperfusion / dextroamphét-amine), on a administré du montélukast par voie intrapéritonéale après les mêmes interventions chirurgicales que pour le groupe 2. Chez tous les rats, une orchidectomie homolatérale a été effectuée en vue d'un examen histologique et de mesures du malonaldéhyde, du glutathion et de la myéloperoxidase dans les tissus. Résultats :Le traitement par montélukast a significativement réduit l'élévation dans les tissus testiculaires provoquée par l'ischémie-reperfusion, mais les taux de malonaldéhyde et de glutathion sont demeurés les mêmes. Le niveau d'activité de la myéloperoxidase (MPO) a significativement augmenté dans les tissus testiculaires du groupe ayant subi l'ischémie-reperfusion sans traitement subséquent. Cependant, dans le groupe ayant subi l'ischémie-reperfusion avec traitement par montélukast, le taux de MPO dans les tissus testiculaires avait significativ...

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Impaired spermatogenesis in testes at risk of torsion

Anderson

Cooper

Thomas

et al. 1986

Journal of British Surgery

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The oligospermia observed after unilateral torsion of the spermatic cord could reflect immunological damage to the opposite testis. An alternative explanation, that there may be a pre-existing defect in spermatogenesis, was tested in 20 post-pubertal males with acute torsion. In a prospective study the contralateral testis was biopsied at operation and the histological appearances were related to subsequent testicular function as assessed by seminal analysis 3 months later. Thirteen patients had biopsy evidence of partial maturation arrest in spermatogenesis which was either mild (6), moderate (4) or severe (3), and ten of these were oligospermic (less than 20 X 10(6)/ml). By contrast all seven patients with normal histology had a sperm concentration greater than 25 X 10(6)/ml. Histological grading of spermatogenesis by the Johnsen technique gave a higher score in patients with a normal biopsy (median 9.01, semi-quartile range 8.96-9.21) than in those with abnormal histology (median 8.28, semi-quartile range 7.98-8.45, P less than 0.001) and correlated with the log of the sperm concentration 3 months later (r = 0.79, P less than 0.001). There was minimal anti-sperm and no anti-testis antibody formation following torsion. Serum FSH levels were raised in 6 of 10 oligospermic patients, while all those with a normal sperm count had FSH concentrations within the normal range. Thus many patients who develop testicular torsion have an underlying defect in spermatogenesis, which correlates closely with poor postoperative exocrine function.

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Histological Changes Occurring in the Contralateral Testes of Prepubertal Rats Subjected to Various Durations of Unilateral Spermatic Cord Torsion

Cited by 164 publications

References 12 publications

Propofol effects on the morphology of rat testes subjected to testicular ischemiareperfusion

Propofol effects on the morphology of rat testes subjected to testicular ischemiareperfusion

Montelukast protects against testes ischemia/reperfusion injury in rats

Impaired spermatogenesis in testes at risk of torsion

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