Histological changes of implanted collagen material during bone induction

Abstract

Studies were made on the fate of implanted material during bone induction. Mixtures of 1 mg of crude bone morphogenetic protein (BMP), or bovine serum albumin as a control, and 1.5 mg of bovine collagen, were pressed into discs and implanted under the fascia of the rectus abdominus muscle of rats. The tissues with implants were fixed 7, 10, and 14 days later and examined histologically. On day 7 after implantation, the implant was surrounded and invaded by alkaline phosphatase-positive cells. New bone and cartilage were seen at the periphery of the implant. In the regions of calcified cartilage and bone, these osteogenic matrices were intermixed with the implant. The mineral deposits were seen by electron microscopy not only on the osteogenic matrices but also on the implanted collagen. On day 14, the bone had spread to the center of the implant. No osteogenesis or chondrogenesis was seen in control implants. It was concluded that the calcification occurred on the implanted collagen during bone induction, and that it was related to successive bone formation and remodeling.

The ability of Ca(OH)2 to facilitate bone formation by measuring the alkaline phosphatase level (Experimental study)

“…Bone defects and regeneration are important clinical considerations in oral and maxillofacial and reconstructive surgery occur in a wide variety of clinical situations, and successful closure of these defects remains as a major concern in the early phase of bone healing as which organization of the clot is completed can be followed clinically, whereas bone remodeling can be determined biochemistry via ALP or radiographically. (13,14) The ALP level express an early sign of osteoblast secretion of matrix which subsequently determined to become osteiod. This activity never been isolated from connective tissue away from bone thus indicated that its source is limited to existing bone.…”

A composite graft material containing bone particles and collagen in osteoinduction in mouse

“…In an experiment similar to ours. Horisaka et al5 used bovine collagen in combination with bovine bone morphogenetic proteins (BMP) to study osteogenesis. They found that the collagen/BMP implant rapidly induced calcification and new bone formation when placed in rat muscle.…”

“…In biomaterial science, collagen is used for sutures, soft‐tissue augmentation, and as a skin substitute 4. Collagen combined with bone morphogenetic proteins (BMP) and implanted into host tissue has an affinity for connective tissue and will gradually degrade and resynthesize to form new bone 5. Blumenthal3 reported his composite DFDBA/collagen graft material to be highly hemostatic, able to expand to fill defects, and well retained when placed into bony lesions of the jaw and maxilla of humans and baboons.…”

Função osteoindutora de fragmentos ósseos conservados em glicerina a 98%: estudo experimental em ratos

“…O termo refere-se ao processo pelo qual as células tronco mesenquimais, presentes no tecido circunjacente ao local do enxerto, são induzidas à diferenciação em células de linhagem osteogênica (ALEXANDER, 1987). Esse mecanismo está relacionado à atividade da "bone morphogenetic protein" (BMP) (URIST, 1965;BESSHO et al, 1992;YABU et al, 1992;OGAWA et al, 1992;BESSHO & IZUKA, 1994;HORISAKA et al, 1994) presente na matriz óssea. Todavia, a preservação dessa proteína e de sua atividade osteoindutora em fragmentos ósseos conservados em glicerina é motivo de dúvida.…”