Histological evaluation of the effects of bone morphogenetic protein 9 and angiopoietin 1 on bone healing

Fadhil, Enas; Dosh, Rasha Hatem; Wally, Zena J.; Haider, Julfikar

doi:10.1016/j.jtumed.2023.02.005

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…Decalcification took place when the needle was inserted. The specimens were washed in running water for 30 min to remove any leftover acid [ 37 ]. Dehydrated by immersing in ascending concentrations of alcohol and clearing was carried out by immersing into two different changes of xylene for 15–20 min.…”

Section: Methodsmentioning

confidence: 99%

Chitosan Nanoparticle/Simvastatin for Experimental Maxillary Bony Defect Healing: A Histological and Histomorphometrical Study

Alsaeed,

Al-Ghaban

2023

Biomimetics

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Biomaterials such as chitosan and simvastatin (Sim) have been introduced to accelerate the extensive and multicellular biological process of bone healing. The aim of this study was to evaluate the bone healing potential of chitosan and Sim, alone or combined. Forty-two male New Zealand rabbits were divided into three groups: chitosan nanoparticles (ChN), Sim and chitosan simvastatin nanoparticles (ChSimN). Two bony defects were created in the maxillary bone. The hole on the right side received one of the experimental materials, while the other side was assigned as the control and left to heal without any intervention. Bone specimens were collected at 2 and 4 weeks and then taken for histological and histomorphometrical analyses. The histological findings revealed that ChN possessed the highest number of osteoblasts and osteoclasts at weeks 2 and osteocytes after 4 weeks. There was a significant difference between the two healing periods regarding all bone parameters across all groups. ChN stood out as the only group that had a significant difference in the count of all bone cells between the two periods, thus having the best potential in promoting bone healing.

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Section: Methodsmentioning

confidence: 99%

Chitosan Nanoparticle/Simvastatin for Experimental Maxillary Bony Defect Healing: A Histological and Histomorphometrical Study

Alsaeed,

Al-Ghaban

2023

Biomimetics

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“…Cutting the bone at least 5mm away from the surgical site allowed for the collection of the samples, which were then fixed in 10% fresh formalin for two days before being decalcified in a solution of formic acid sodium citrate (125cc of 90% formic acid, 125cc of distilled water, 50mg sodium citrate, and 250cc of distilled water) [10] . The specimens were checked using a needle and ease of inserting the needle was used as an indicator of good decalcification [11]. This was followed by dehydration in ascending concentrations of alcohol, clearing in two different changes of xylene, and blocking.…”

Section: Surgical Proceduresmentioning

confidence: 99%

The influence of Simvastatin carried by Chitosan nanoparticle on bone regeneration using Masson’s Trichrome histochemical stain

Alsaeed,

Al-Ghaban,

Karaibrahimoğlu

2023

J Bagh Coll Dent

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Background: Due to the complicated and time-consuming physiological procedure of bone healing, certain graft materials have been frequently used to enhance the reconstruction of the normal bone architecture. However, owing to the limitations of these graft materials, some pharmaceutical alternatives are considered instead. Chitosan is a biopolymer with many distinguishing characteristics that make it one of the best materials to be used as a drug delivery system for simvastatin. Simvastatin is a cholesterol lowering drug, and an influencer in bone formation process, because it stimulates osteoblasts differentiation, bone morphogenic protein 2, and vascular endothelial growth factor. Objectives: histological, histochemical and histomorphometrical analyses were carried out to evaluate the effect of local application of chitosan simvastatin nanoparticles (ChSimN) on bone healing. Materials and Methods: New Zealand rabbits (n=14) were used in this study. Two defects were made: one on the right side (the experimental side) that received ChSimN and the other one on the left side (the control side), which left to heal spontaneously. Seven rabbits were sacrificed after 2 weeks of the experiments, while the others after 4 weeks. Bone samples were taken for histological and histomorphometric study after the sacrifice. Results: The histological study, using both H&E and Masson’s Trichrome stain, revealed that the ChSimN group recorded an increased amount of bone formation at both time points. Histomorphometrical analysis recorded a significant increment in bone marrow and trabecular areas in the ChSimN group. Conclusion: ChSimN had a pronounced effect on bone formation.

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“…Researchers have been developing alternative treatment methods for delayed fracture healing for many years. Numerous approaches have been investigated to accelerate maturation of regenerated bone, such as growth factors [6], calcitonin [7], calcium sulfate [8], bisphosphonates [9], electronic [10] and ultrasonic [11] stimulation. Various factors are defined that support healing after fractures such as interleukins (IL); IL-1, IL-6, some growth factors; fibroblast, transformed and platelet-derived growth factors [12,13].…”

Section: Introductionmentioning

confidence: 99%

Effects of local application of bovine amniotic fluid on fracture healing in rats (Rattus norvegicus)

Tanrısever,

Istek,

Eroksuz

et al. 2024

RC FCV-LUZ

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In this study, it was aim to examine the local application of bovine amniotic fluid on bone fracture healing in rats. Twenty female sprague dawley rats included in the study were divided into 2 groups of 10. The sham group (n=10): Bone fractures were created in the right tibia bones of the rats and fixed with kirschner wire. After a four–week recovery period, the subjects were sacrificed. Local bovine amniotic fluid group (n=10): Bone fractures were created in the right tibia bones of the rats and local bovine amniotic fuid was applied during fixation with kirschner wire. After a four–week recovery period, the subjects were sacrificed. Samples from all subjects were decalcified, stained with hematoxylin and eosin, and new bone formation and fibrosis were analyzed. When the groups were evaluated in terms of new bone regeneration, it was determined that the new bone regeneration in the subjects treated with local bovine amniotic fluid were statistically significantly higher than sham group (P<0.05). When the groups were evaluated in terms of fibrosis, the fibrosis value in the sham group was found to be statistically significantly higher when compared with the local bovine amniotic fluid group (P<0.05). It can be stated that local bovine amniotic fluid application may positively affect the healing of bone fractures.

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Histological evaluation of the effects of bone morphogenetic protein 9 and angiopoietin 1 on bone healing

Cited by 4 publications

References 36 publications

Chitosan Nanoparticle/Simvastatin for Experimental Maxillary Bony Defect Healing: A Histological and Histomorphometrical Study

Chitosan Nanoparticle/Simvastatin for Experimental Maxillary Bony Defect Healing: A Histological and Histomorphometrical Study

The influence of Simvastatin carried by Chitosan nanoparticle on bone regeneration using Masson’s Trichrome histochemical stain

Effects of local application of bovine amniotic fluid on fracture healing in rats (Rattus norvegicus)

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