Histological study of somatic embryogenesis induction on zygotic embryos of macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius)

Moura, Elisa Ferreira; Ventrella, Marília Contin; Motoike, Sérgio Yoshimitsu; Júnior, Adauto Quirino de Sá; Carvalho, Mychelle; Manfio, Cândida Elisa

doi:10.1007/s11240-008-9430-9

Cited by 52 publications

(37 citation statements)

References 34 publications

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“…In multicellular origin, somatic embryos are histologically fused with the mother tissue and could also fuse with other somatic embryos (Moura et al 2008). This process of origin was most frequent in A. aculeata, but these embryos did not regenerate plants (Moura et al 2008). In the present study, somatic embryos showed the same multicellular origin, which could explain the low conversion rate.…”

Section: Discussionsupporting

confidence: 43%

“…In zygotic embryos, somatic embryos formed close to procambial strands of calli and had two origins: unicellular and multicellular. In multicellular origin, somatic embryos are histologically fused with the mother tissue and could also fuse with other somatic embryos (Moura et al 2008). This process of origin was most frequent in A. aculeata, but these embryos did not regenerate plants (Moura et al 2008).…”

Section: Discussionmentioning

confidence: 99%

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Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

et al. 2015

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Considering the necessity of Acrocomia aculeata propagation for large-scale production, the aim of this study was to establish a somatic embryogenesis protocol using the thin cell layer (TCL) technique. Aerial parts of in vitro plants were transversally cut at the base into eight TCLs and placed in a culture medium for callus induction. The induction medium was composed of Y3 salts and Morel´s vitamins and supplemented with 150, 300 or 600 μM picloram. After 12 weeks the calli were transferred to a medium supplemented with BAP or 2-iP (12.5 or 25 μM). After 18 weeks, the somatic embryo clusters were transferred to a conversion medium (plant growth regulator-free medium). Primary callus induction rate was higher in the first three TCLs and in media containing 150 or 300 μM picloram. The best maturation results were obtained in medium containing 12.5 μM 2-iP or 12.5 μM BAP. Few somatic embryos converted into plants. The histological analyses showed that callus induction started adjacent to vascular bundles after two days of culture, and somatic embryos arose in the periphery of nodular calli. This study showed that the TCL embryogenesis protocol is promising for in vitro multiplication of A. aculeata.

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Section: Discussionsupporting

confidence: 43%

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

et al. 2015

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“…During the indirect somatic embryogenesis different types of callus are frequently produced, some of which have potential to generate plants. Identification of callus cells with embryogenic potential can be achieved through cytological and ultrastructural analysis (Fillipi et al 2001, Steiner et al 2005, Moura et al 2008, thereby greatly enhancing the efficiency of the in vitro propagation process.…”

Section: Introductionmentioning

confidence: 99%

Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses

Pádua

Paiva

Labory

et al. 2013

An. Acad. Bras. Ciênc.

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Oil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses in E.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in ). After 90 days the presence/ absence of cell masses were evaluated. Both growth regulators efficiently induced cellular masses regardless of the concentrations applied. As the cell masses were not homogeneously formed, they were classified according to color and shape into four types: TYPE 1 -elongated and translucent, TyPE 2 -uneven and translucent, TyPE 3 -globular and beige TyPE 4 -globular and white. Based on the anatomical and ultrastructural features, TyPE 2, 3 and 4 cell masses were considered to have the highest embryogenic potential and therefore may be most suited to large-scale vegetative propagation of oil palm.

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“…Propagation is usually carried out through seeds, but the development and improvement of in vitro propagation techniques are necessary to speed up and facilitate genetic breeding programs. Somatic embryos in vitro were recently obtained (Moura et al, 2008(Moura et al, , 2009, but the rate of plantlets conversion is still low.…”

Section: Introductionmentioning

confidence: 99%

Anatomy, histochemistry and ultrastructure of seed and somatic embryo of Acrocomia aculeata (Arecaceae)

Moura¹,

Ventrella²,

Motoike³

2010

Sci. agric. (Piracicaba, Braz.)

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103

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Macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Mart.) is a tropical species with multiple uses, including oil supply for biodiesel production. However, structural and physiological studies of the seed are still scarce, as well as in vitro propagation techniques. The aim of this study was to characterize the anatomy, histochemistry and ultrastructure of the seed and the in vitro somatic embryo of A. aculeata, gaining insight into relationships between the post-harvest seed behavior and the somatic embryo conversion to plant. The zygotic embryo and the endosperm show high quantities of protein and lipids, stored in protein and lipid bodies, respectively. The cell wall storage polysaccharides occur in the thickened cell wall of the endosperm. The absence of vacuoles and the few organelles, except for the storage ones, indicate low metabolic state of the zygotic embryo, which suggests a orthodox behavior of the seed at maturity. The somatic embryo shows a shoot meristem with few leaf primordia, vacuolated cells and, occasionally, amyloplasts , but not lipid or protein reserves. These characteristics, common in somatic embryogenesis, could be associated with the maturation phase, and, consequently, with the low conversion of these embryos into plants. Key words: macaw palm, zygotic embryo, endosperm, storage reserves Anatomia, histoquímica e ultra-estrutura da semente e do embrião somático de Acrocomia aculeata (Arecaceae)RESUMO: A macaúba (Acrocomia aculeata (Jacq.) Lodd. ex Mart.) é uma espécie tropical com múltiplos usos, incluindo a geração de óleo para a produção de biodiesel. Entretanto, estudos estruturais e fisiológicos da semente ainda são escassos, assim como as técnicas de propagação in vitro. Caracterizaram-se a anatomia, a histoquímica e ultraestrutura da semente e do embrião somático de A. aculeata obtido in vitro, visando a obter relações entre o comportamento pós-colheita da semente e a conversão em planta a partir do embrião somático. O embrião zigótico e o endosperma apresentam alta quantidade de proteínas e lipídios, estocados em corpos protéicos e lipídicos, respectivamente. Os polissacarídeos de reserva da parede celular estão presentes nas paredes celulares espessadas do endosperma. A ausência de vacúolos e as poucas organelas visualizadas, exceto as organelas de reserva, indicam baixo estado metabólico do embrião zigótico, o que sugere comportamento ortodoxo da semente madura. O embrião somático apresenta meristema apical com poucos primórdios foliares, células com vacúolos conspícuos e, ocasionalmente, amiloplastos, mas não há reservas protéicas ou lipídicas. Essas características, comuns na embriogênese somática, podem estar associadas à fase de maturação dos embriões e, consequentemente, com a baixa conversão desses embriões em plantas. Palavras-chave: macaúba, embrião zigótico, endosperma, substâncias de reserva

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Histological study of somatic embryogenesis induction on zygotic embryos of macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius)

Cited by 52 publications

References 34 publications

Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses

Anatomy, histochemistry and ultrastructure of seed and somatic embryo of Acrocomia aculeata (Arecaceae)

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