Histological study of surface modified three dimensional poly (d, l-lactic acid) scaffolds with chitosan in vivo

Abstract

Biocompatibility and tissue regenerating capacity are essential for biomaterials that used in tissue engineering. The aim of this study was to histologically assess the tissue reactions and bone conductivities of surface modified three dimensional (3-D) poly (D, L-lactic acid) (PDLLA) scaffolds, which were coated with chitosan via a physical entrapment method. The native PDLLA scaffold was prepared via thermally induced phrase separation technique and was characterized by scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). Osteocalcin assay, a method to evaluate the bone formation potential, has shown that the osteocalcin production in chitosan-modified 3-D PDLLA scaffold group was significantly higher (p < 0.05) than that of in control. The tissue reactions and bone conductivities between surface modified PDLLA and native PDLLA scaffolds were evaluated using a rabbit radialis defect model in vivo and compared at different implantation intervals (2, 4, 8 and 12 weeks). The histological results have shown a higher bone formation potential and better biocompatibility of chitosan-modified 3-D PDLLA scaffolds as compared with the control group scaffolds.

Biomedical applications of biodegradable polymers

“…Instead PDLLA has been commonly used as a drug delivery film for inorganic implants, [73][74][75][76] or as a tissue engineering scaffold. [77][78][79] Like PLLA, PDLLA has been often combined with other degradable polymers such as PLGA, 80 PEG, 81,82 and chitosan 83 to create composites with desirable material properties.…”

Cockle Shell-Based Biocomposite Scaffold for Bone Tissue Engineering

“…In addition, the bone forming capacity of these transplants have not been solved after discontinuation of immunosuppressive. The surface of chitosan modified PDLLA scaffolds study was conducted by Cai et al (2007) after implantation for 12 weeks. A lot of new bone was formed within the scaffolds at this stage with a mild inflammatory reaction.…”

Erratum: Buzdar AU, Guastalla J‐P, Nabholtz J‐M, et al. Impact of chemotherapy regimens prior to endocrine therapy: results from the ATAC (Anastrozole and Tamoxifen, Alone or in Combination) trial. Cancer. 2006; 107(3):472–80.

“…Instead PDLLA has been commonly used as a drug delivery film for inorganic implants,73–76 or as a tissue engineering scaffold 77–79. Like PLLA, PDLLA has been often combined with other degradable polymers such as PLGA,80 PEG,81, 82 and chitosan83 to create composites with desirable material properties.…”