Histomorphometric analysis and immunolocalization of RANKL and OPG during the alveolar healing process in female ovariectomized rats treated with oestrogen or raloxifene

Luvizuto, Eloá Rodrigues; Queiroz, Thallita Pereira; Dias, Sheila Mônica Damásio; Okamoto, Tetuo; Dornelles, Rita Cássia Menegati; Garcia, Idelmo Rangel; Okamoto, Roberta

doi:10.1016/j.archoralbio.2009.11.001

Cited by 48 publications

(53 citation statements)

References 42 publications

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“…A previous study 36 reported intense and moderate RANKL staining on osteoblasts and osteoclasts. Positive BSP staining was also previously reported 12 during the first week after ovariectomy and OCC expression was clearly changed by ovariectomy 37 .…”

Section: Discussionsupporting

confidence: 79%

Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle

Salgado¹,

Marchini²,

Tera³

et al. 2015

Braz. J. Oral Sci.

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Estrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided equally into two groups: ovariectomized (Ovx) and simulated ovariectomy (Sham). One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein (BSP), osteocalcin (OCC) and receptor activator of nuclear factor kappa-B ligand (RANKL). Results: Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups (p=0.049). No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups.

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Section: Discussionsupporting

confidence: 79%

Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle

Salgado¹,

Marchini²,

Tera³

et al. 2015

Braz. J. Oral Sci.

View full text Add to dashboard Cite

show abstract

“…These foregoing findings was in agreement with other studies (39) , which found that the alveolar repair process associated with the receptor activator of NF-κB ligand and OPG pattern being inversely proportional. As there was an increase in bone formation during the course of the analyzed periods, while there was a reduction in the expression of these proteins, indicating complete filling of the alveolar process associated with a balance in bone turnover.…”

Section: Discussionsupporting

confidence: 93%

Evaluation of the Effect of Platelet Rich Fibrin on Alveolar Socket Healing in Glucocorticoids-Induced Osteoporosis in Rabbits (Histological and Immuno-histomorphometric study)

2017

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Purpose: The purpose of this study was to evaluate the effect of platelet rich fibrin on the healing of osteoporotic alveolar socket after tooth extraction in experimentally induced osteoporosis in rabbits. Materials and Methods: Thirty six adult female rabbits were used in this study. Rabbits were divided into 3 groups (group1, group2 &group 3: 12 for each group) then each group was divided into 3 subgroups (4 for each subgroups) according to the date of scarification at 7, 20, 30 days postoperatively. Group 1: (12 rabbits), alveolar socket was left empty after first molar extraction (didn't receive Platelet Rich Fibrin (PRF)). For the remaining 24 female rabbits: osteoporosis was experimentally induced by receiving 1.56 mg/kg dexamethasone (Dex) intraperitoneal injection daily for 2 months to induce osteoporosis -like condition. Group 2: (12 rabbits), osteoporotic alveolar sockets were left empty after first molar extraction (didn't receive PRF). Group 3: (12 rabbits), osteoporotic alveolar socket received PRF after first molar extraction. The specimens were collected, prepared and examined by routine haematoxylin &eosin and immunostained by Osteoprotogrin (OPG) marker. Results: at 7, 20 and 30 days, the greatest mean value was recorded in group 1, followed by group 3, with the least value recorded in group 2. Both group 1and 3 revealed a decrease in area percent of OPG throughout the study, whereas in group 2 revealed an increase. Conclusions: The study offers a clear evidence that topical application of PRF in osteoporotic alveolar socket after tooth extraction accelerates and increases the rate of bone formation and maturation than in osteoporotic alveolar socket without PRF.

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“…Comparatively, Luvizuto et al 26 studied RANK-L and OPG during the alveolar healing process in female ovariectomized rats treated with estrogen or raloxifene. They found that both studied proteins were immunodetected in osteoblasts, osteocytes and/or in bone lining cells, which are the predominant cells at the studied stages of alveolar bone healing at 7, 14, 21, 28 and 42 days.…”

mentioning

confidence: 99%

The RANK/ RANKL/ OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency

et al. 2014

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The aim of this study was to evaluate the resorption process during the repair of autogenous bone grafts with or without coverage by an expanded polytetrafluoroethylene (e-PTFE) membrane in female rats with estrogen deficiency using the immunohistochemical technique. Eighty female rats were randomly divided into two groups (OVX and SHAM). The 40 female rats in the OVX group were subjected to ovariectomy, and the 40 female rats in the SHAM group were subjected to simulated ovariectomy. The two groups were further divided in subgroup E, which was subjected to surgery for placement of autogenous bone graft (ABG), and subgroup ME, in which the ABG was covered with an e-PTFE membrane. The animals were killed at 0, 7, 21, 45 and 60 days. The specimens were analyzed using immunohistochemistry for the bone resorption markers RANK, RANK-L and Osteoprotegerin (OPG). A higher remodeling rate was observed at 7 and 21 days after the autogenous bone grafts, when the markers were more intensely expressed. At the final time point, the specimens presented similar characteristics to those observed at the initial time point. The expression of immunohistochemical markers was not altered by the estrogen deficiency. The presence of the e-PTFE membrane delayed the bone resorption process, influencing the immunohistochemical expression of markers.

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Histomorphometric analysis and immunolocalization of RANKL and OPG during the alveolar healing process in female ovariectomized rats treated with oestrogen or raloxifene

Cited by 48 publications

References 42 publications

Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle

Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle

Evaluation of the Effect of Platelet Rich Fibrin on Alveolar Socket Healing in Glucocorticoids-Induced Osteoporosis in Rabbits (Histological and Immuno-histomorphometric study)

The RANK/ RANKL/ OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency

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