Histomorphometric Estimation of Age From Bone Samples  of Nigerians

Orupabo, Clinton D.; Oghenemavwe, Loveday Ese

doi:10.11603/ijmmr.2413-6077.2020.2.11414

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…It has been suggested that bone tissues with more primary osteons may be mechanically stronger, as primary osteons have smaller lamellae and vascular channels [35]. Apart from sex, our previous study showed that primary osteons correlated inversely with age [36].…”

Section: Sex Predicted Group Membership Totalmentioning

confidence: 94%

Sex Estimation From Histomorphometric Analysis of Cortical Bone: A Hospital-based Study

Oghenemavwe

Orupabo

2022

AJFSFM

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Estimation of the sex of unknown skeletal remains is a daunting challenge, when only fragmented bones are available. For this reason, there is a need to study population specific histomorphometric features of bones to provide useful data for the process of identification. The purpose of this study was to estimate sex from six histomorphometric parameters of cortical bones in a cohort of Nigerian patients. The samples comprised non-pathologic bones collected from 29 patients (12 females and 17 males) between the ages of 35 to 85 years during orthopaedic procedures. The modified Frost’s manual method was used to prepare the histological sections of the cortical bones. The parameters evaluated were number of primary osteons (Os-p), number of secondary osteons (Os-s), osteon fragments (Os-f), non-Haversian canal (N-Hc), area of Haversian canal (Area Hc), and Haversian canal diameter (HCD). The data obtained were analyzed using SPSS version 23.0. Tests for sex differences were done using student t-test and stepwise discriminant function was used to formulate a predictive model for sex estimation. The mean Os-p, Os-s, Os-f, N-hc, Area HC, and HCD were 2.85 ± 0.86,3.92 ± 1.26,6.08 ± 1.37, 2.23 ± 0.66, 53.43 ± 5.55, and 7.85 ± 0.41 for males and 1.11 ± 0.56, 0.33 ± 0.17, 2.44 ± 0.67, 54.37 ± 6.04 and 7.426 ± 0.35 for females, respectively. The mean value of Os-s was significantly different in males and females (p=0.04). Stepwise discriminant function analysis showed Os-s could be used to estimate sex. 71.4% of our samples were correctly predicted based on sex. Discriminant function values of 0.418 and -0.626 were predictive values for males and females, respectively, using the DFA predictive model. Our study shows that the number of secondary osteons (Os-s) could be used to estimate sex in Nigerian patients.

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Section: Sex Predicted Group Membership Totalmentioning

confidence: 94%

Sex Estimation From Histomorphometric Analysis of Cortical Bone: A Hospital-based Study

Oghenemavwe

Orupabo

2022

AJFSFM

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Heat-induced changes in the histomorphometry of human femur bone

Oghenemavwe,

Orupabo,

Praise

2024

Advances in Biomedical and Health Sciences

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Background: Skeletal remains are often subjected to unexpected and adverse conditions which can eventually distort evidence. Therefore, understanding the factors that alter skeletal remains is crucial. Methods: A total of human femur fragments obtained from the gross anatomy laboratory of the University of Port Harcourt were used. Bone samples were divided into four groups. Group 1 is the control sample comprising bone fragments not exposed to heat, Group 2 had bones exposed to heat at 20°C, Group 3 had bones exposed to heat at 100°C, and Group 4 had bones exposed to heat at 200°C. Ground bone sections were prepared using Modified Frost’s manual method of bone preparation. Morphometric analysis of the micrographs was performed using the ImageJ software. Results: Data were analyzed using the SPSS software. The mean values of the Haversian canal area and diameter demonstrated a nonsignificant increase in the bone samples exposed to heat compared to the samples not exposed to heat. The osteon count was slightly higher, but the difference was not statistically significant (P > 0.05). Conclusion: The results demonstrated that histomorphometric features of bones are not altered at the temperature of 200°C. This information is useful in forensic investigations.

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A Comparative Analysis on the Histomorphometric Pattern of Various Segments of Adult Male Long Bones: Forensic Implication

Orupabo,

Chijioke

2024

Journal of Forensic Science and Medicine

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Background: Human bone analysis is a useful tool in assessing and identifying skeletal remains. To make this easier, newer and modified approaches have often being documented. Aims and Objectives: The aim of this study is to analyze the histomorphometric features of various bone segments and its relevance in forensic case diagnosis. Materials and Methods: The human bones used were that of cadaveric bone samples harvested from three adult male Nigerians. The proximal, midshaft and distal segments of the femur, tibia, and humerus were utilized for the study. The bone sections were subjected to processing using Frost’s rapid manual preparation of ground sections techniques. Histomorphometric evaluation studied the number of primary osteons, secondary osteons, and osteon fragments, as well as the Haversian canal diameter (HCD). Results: The primary osteons, secondary osteons, osteon fragments, and HCD in each of the segments of the long bones show that the F values are less than the F critical for an alpha level of 0.05. This shows that there is no statistically significant (P > 0.05) variation in the histomorphometric pattern for the various segments of the bones studied. Again differences in the haversian canal diameter of the various segments of the long bones shows statistically significant variation (P< 0.05) among the long bones investigated. Conclusion: These findings have laid to rest the challenges with using the only available bone fragment for forensic case investigation as no notable variations exist across various segments.

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Histomorphometric Estimation of Age From Bone Samples of Nigerians

Cited by 3 publications

References 9 publications

Sex Estimation From Histomorphometric Analysis of Cortical Bone: A Hospital-based Study

Sex Estimation From Histomorphometric Analysis of Cortical Bone: A Hospital-based Study

Heat-induced changes in the histomorphometry of human femur bone

A Comparative Analysis on the Histomorphometric Pattern of Various Segments of Adult Male Long Bones: Forensic Implication

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