Histomorphometric study of the effect of methionine on small intestine parameters in rat: an applied histologic study

Seyyedin, Sajad; Nazem, Mohammad Naser

doi:10.5603/fm.a2017.0044

Cited by 36 publications

(34 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The third segment of the small intestine of the animals was the ileum which appeared the shortest part of the small intestine. It joined the jejunum cranially and extended caudally to join the cecum these result agreement with other studies such as (14,17) .…”

Section: Resultssupporting

confidence: 93%

“…The cone had centripetal coils, a sigmoid flexure and centrifugal coils. This jejunum shape was similar to other studies in squirre (17) .…”

Section: Resultssupporting

confidence: 91%

“…The small intestine appeared long convoluted tube, extends from the junction with stomach to joining point with the large intestine. Three segments were found in the small intestine of the studied animals, namely duodenum, jejunum and ileum these result agreement with other studies such as (15,17) .…”

Section: Histological Aspectsupporting

confidence: 92%

See 2 more Smart Citations

Histo-morphomertic, Histochemical Solidity Ruling of the Small Intestine in Hamsters and Rabbit According to Different Food

2021

IJFMT

View full text Add to dashboard Cite

In the present study, the histo-morphometric comparison study of small intestine in hamsters and rabbit was aimed. Ten of clinically healthy animals of both sexes were collected by Al-Simawa city, age ranged between 6 month to 1 year, which was estimated according to the dental equation of the animals. The small intestine was cut and stained alternatively with Hematoxylin and Eosin (H & E) and (Lilles Alchrome stain).The current study aims to discover the structures and measurement of small intestine belonging to these animals specifically their histo-morphometric textures. The anatomical studies revealed that small intestine of both animals consists of: duodenum, jejunum and ileum. Histological exam was showed that the wall of small intestine composed of four layers including: mucosa, submucosa, muscularis and serosa and adventitia. The epithelia of villi small intestine were simple columnar cells for each animal.

show abstract

Section: Resultssupporting

confidence: 93%

“…The cone had centripetal coils, a sigmoid flexure and centrifugal coils. This jejunum shape was similar to other studies in squirre (17) .…”

Section: Resultssupporting

confidence: 91%

See 1 more Smart Citation

Histo-morphomertic, Histochemical Solidity Ruling of the Small Intestine in Hamsters and Rabbit According to Different Food

2021

IJFMT

View full text Add to dashboard Cite

show abstract

“…The intestinal villus height in zebrafish tissues and the ratio of villus height to crypt depth in rat tissues, the percentage of blunted villi and percentage of erosion or ulcer were analyzed with H&E‐stained sections. In addition to, goblet cells per villus in 10 well‐oriented villi were counted with PAS‐stained sections (Seyyedin & Nazem, ).…”

Section: Methodsmentioning

confidence: 99%

Development of an alternative zebrafish model for drug‐induced intestinal toxicity

Ryu

Kim

et al. 2017

J of Applied Toxicology

View full text Add to dashboard Cite

An evaluation of intestinal toxicity is important because the mucosal lining of the gastrointestinal tract is the first barrier for oral xenobiotics. Until now, a rat model has been recommended as the standard intestinal toxicity model and the Caco-2 cell line, originated from a human colon adenocarcinoma, has been used as an alternative to this model, but there are limitations regarding cost-effectiveness and the need for mimicry of the human system. In this study, we investigated whether zebrafish could be a valid alternative to rats and Caco-2 cells as an intestinal toxicity model. We focused on intestinal gene expression of cytochrome P450 3A65, oxidative stress, apoptosis, inflammation, and intestinal function. Reverse transcription-quantitative polymerase chain reaction analysis was conducted using three models: zebrafish, Sprague-Dawley rats and Caco-2 cells, and the transcript levels and patterns of indicator genes were analyzed in conjunction with histopathological changes. Our results suggested that representative intestinal toxicants, indomethacin, diclofenac and methotrexate, induced significant transcript level changes in marker genes such as CYP3A, inducible nitric oxide synthase, heme oxygenase 1, superoxide dismutase 1, glutathione peroxidase 1, BCL2 associated X, B-cell lymphoma 2, caspase 9, tumor protein p53, nuclear factor-κB, interleukin-1β, tumor necrosis factor-alphaα and toll-like receptor 2 in the zebrafish model as in the rat and Caco-2 cells models. These results suggest that zebrafish model is sufficiently worth developing as an intestinal toxicity model that can replace or compensate the rat model or Caco-2 cell model.

show abstract

“…Forty adult male and female Wistar rats (9-10 weeks of age) with no significant difference in their body weight (251.59±12.31 g) were randomly divided into four equal groups. All rats were allowed a 5-day adaptation period in a room with controlled conditions (temperature 22-25 °C and humidity 60-70 %) before starting the experiment (Seyyedin and Nazem, 2017). Ten of each sex were selected as male and female control groups while running exercises were performed in remained 20 male and female rats for 6 weeks on a motor-driven rodent treadmill (Model T510, DRI Co., Taiyuan, Taiwan), speed of 20 m/min with inclination of 18° for 60 minutes each day on 5 days of every week.…”

Section: Methodsmentioning

confidence: 99%

Histomorphometric evaluation of tibial subchondral bone after moderate running in male and female Wistar rats

Nazem

Sahebozamani²,

Naeini³

et al. 2021

Folia Morphol

Self Cite

View full text Add to dashboard Cite

Exercise has been shown to be beneficial to the skeleton, in both humans and animals. This study was done to test the sex-related difference in the risk of OA (osteoarthritis) of the knee joint and also on the subchondral bone after moderate running exercise.Forty male and female Wistar rats were randomly assigned to four equal groups (2 male and 2 female groups) in a same condition. Ten of each sex were selected as control groups, while running exercises were performed in remained 20 male and female rats using a motor treadmill to motivate rats to run daily distances of 1 km at 5 days/week within six weeks.On day 43, all animals were sacrificed and the knee articular cartilage and also histomorphometric parameters of subchondral bone and mid shaft of tibia were evaluated.Results showed mild OA in both male and female runner groups. Results in male runner rats were significantly lesser than that in female runners. On the other hand, the difference 2 in female runner group showed significant changes in comparison with other groups in histomorphometric parameters in tibia.Obtained results showed that the development of knee OA and subchondral bone changes may be related to the sex differences. Although there was no synovitis in male runners but female runner group showed mild hyperemia dropsy with a moderate synovitis in this region.

show abstract

Histomorphometric study of the effect of methionine on small intestine parameters in rat: an applied histologic study

Cited by 36 publications

References 43 publications

Histo-morphomertic, Histochemical Solidity Ruling of the Small Intestine in Hamsters and Rabbit According to Different Food

Histo-morphomertic, Histochemical Solidity Ruling of the Small Intestine in Hamsters and Rabbit According to Different Food

Development of an alternative zebrafish model for drug‐induced intestinal toxicity

Histomorphometric evaluation of tibial subchondral bone after moderate running in male and female Wistar rats

Contact Info

Product

Resources

About