Histone acetylation regulates BMMCs recognition of foot-and-mouth disease virus-like particles

Zhang, Junjuan; Han, Weijian; Li, Mingzhu; Bai, Ruoman; Tian, Zhanyun; Yuan, Wanzhe; Li, Limin

doi:10.1016/j.intimp.2023.110428

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…The mice were sacrificed by cervical dislocation. The BMMCs were obtained as described previously [16,35]. The BMMCs were cultured in a complete RPMI 1640 medium (Gibco, Grand Island, NY, USA), and supplemented with 10% fetal bovine serum, 100 U/mL penicillin/streptomycin (Gibco, Grand Island, NY, USA), 10 ng/mL recombinant murine IL-3 (rIL-3, Novoprotein, Suzhou, China), and 20 ng/mL recombinant murine stem cell factor (SCF) (rSCF, Novoprotein, Suzhou, China) at 37 • C under 5% CO 2 .…”

Section: Cell Samples Preparationmentioning

confidence: 99%

“…Activated MCs can immediately release a unique panel of biologically active mediators, regulating innate immunity and adaptive immunity against bacterial and viral infection [12][13][14]. Our previous study demonstrated that MCs can recognize FMDV-VLPs or the recombinant FMDV VP1-VP4 protein to secrete various differentially expressed cytokines via mannose receptors [15,16]. Compared with the control group, the levels of IL-1α, IL-2, IL-4, IL-15, IL-17A, IL-21, TNF-α, IFN-γ, CCL-17, and CCL21 of the VLP group were all significantly upregulated (p < 0.05) and IL-10 showed no difference, while the levels of IL-1α, IL-2, IL-4, IL-15, IL-17A, IL-21, TNF-α, IFN-γ, CCL-17, and L-selectin of the iMR-VLP group were significantly downregulated (p < 0.05).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Chromatin Accessibility Changes Induced by BMMC Recognition of Foot-and-Mouth Disease Virus-like Particles through ATAC-seq

Han,

Zhang,

et al. 2023

IJMS

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Mast cells can recognize foot-and-mouth disease virus-like particles (FMDV-VLPs) via mannose receptors (MRs) to produce differentially expressed cytokines. The regulatory role of chromatin accessibility in this process is unclear. Bone marrow-derived mast cells (BMMCs) were cultured, and an assay of transposase-accessible chromatin sequencing (ATAC-seq) was applied to demonstrate the regulation of chromatin accessibility in response to the BMMCs’ recognition of FMDV-VLPs. A pathway enrichment analysis showed that peaks associated with the nuclear factor-κB (NF-κB), mitogen-activated protein kinase (MAPK), phosphatidylinositol 3 kinase-protein kinase B (PI3K-Akt), and other signaling pathways, especially the NF-κB pathway, were involved in the BMMCs’ recognition of VLPs. Moreover, transcription factors including SP1, NRF1, AP1, GATA3, microphthalmia-associated transcription factor (MITF), and NF-κB-p65 may bind to the motifs with altered chromatin accessibility to regulate gene transcription. Furthermore, the expression of NF-κB, interleukin (IL)-9, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ in the BMMCs of the VLP group increased compared with that of the BMMCs in the control group, whereas the expression of IL-10 did not differ significantly between groups. After inhibiting the MRs, the expression of NF-κB, IL-9, TNF-α, and IFN-γ decreased significantly, whereas the expression of IL-10 increased. The expression of MAPK and IL-6 showed no significant change after MR inhibition. This study demonstrated that MRs expressed on BMMCs can affect the NF-κB pathway by changing chromatin accessibility to regulate the transcription of specific cytokines, ultimately leading to the differential expression of cytokines. These data provide a theoretical basis and new ideas for the development of a novel vaccine for FMD.

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Section: Cell Samples Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Chromatin Accessibility Changes Induced by BMMC Recognition of Foot-and-Mouth Disease Virus-like Particles through ATAC-seq

Han,

Zhang,

et al. 2023

IJMS

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“…Histone deacetylation, one of the post-translational modifications, has been reported to be significant in viral infection [ 20 , 21 ]. Histone acetylation regulates the immune response induced by the bone marrow-derived mast cell recognition of FMDV virus-like particles [ 22 ]. ID1 inhibits the transcriptional activity of FOXO1 through HDAC4-mediated deacetylation, thus promoting IFN-I production and antiviral immune responses.…”

Section: Introductionmentioning

confidence: 99%

Foot-and-Mouth Disease Virus Capsid Protein VP1 Antagonizes Type I Interferon Signaling via Degradation of Histone Deacetylase 5

Gong,

Ren,

Dou

et al. 2024

Cells

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Foot-and-mouth disease (FMD) is a highly contagious and economically important disease of cloven-hoofed animals that hampers trade and production. To ensure effective infection, the foot-and-mouth disease virus (FMDV) evades host antiviral pathways in different ways. Although the effect of histone deacetylase 5 (HDAC5) on the innate immune response has previously been documented, the precise molecular mechanism underlying HDAC5-mediated FMDV infection is not yet clearly understood. In this study, we found that silencing or knockout of HDAC5 promoted FMDV replication, whereas HDAC5 overexpression significantly inhibited FMDV propagation. IFN-β and IFN-stimulated response element (ISRE) activity was strongly activated through the overexpression of HDAC5. The silencing and knockout of HDAC5 led to an increase in viral replication, which was evident by decreased IFN-β, ISG15, and ISG56 production, as well as a noticeable reduction in IRF3 phosphorylation. Moreover, the results showed that the FMDV capsid protein VP1 targets HDAC5 and facilitates its degradation via the proteasomal pathway. In conclusion, this study highlights that HDAC5 acts as a positive modulator of IFN-β production during viral infection, while FMDV capsid protein VP1 antagonizes the HDAC5-mediated antiviral immune response by degrading HDAC5 to facilitate viral replication.

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Nanoparticulate chitosan-TNF-α-VLPs activate mast cells and enhance adaptive immunity induced by foot-and-mouth disease virus-like particles in mice

Bai,

Li,

Tian

et al. 2023

Veterinary Immunology and Immunopathology

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Histone acetylation regulates BMMCs recognition of foot-and-mouth disease virus-like particles

Cited by 5 publications

References 37 publications

Analysis of Chromatin Accessibility Changes Induced by BMMC Recognition of Foot-and-Mouth Disease Virus-like Particles through ATAC-seq

Analysis of Chromatin Accessibility Changes Induced by BMMC Recognition of Foot-and-Mouth Disease Virus-like Particles through ATAC-seq

Foot-and-Mouth Disease Virus Capsid Protein VP1 Antagonizes Type I Interferon Signaling via Degradation of Histone Deacetylase 5

Nanoparticulate chitosan-TNF-α-VLPs activate mast cells and enhance adaptive immunity induced by foot-and-mouth disease virus-like particles in mice

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