Histone acetyltransferase GCN5 interferes with the miRNA pathway in Arabidopsis

Kim, Wanhui; Benhamed, Moussa; Servet, Caroline; Latrasse, David; Zhang, Wei; Delarue, Marianne; Zhou, Dao‐Xiu

doi:10.1038/cr.2009.59

Cited by 75 publications

(55 citation statements)

References 58 publications

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“…In turn, miRNA transcription could be influenced by the altered epigenetic marks surrounding the miRNA genes (Rodriguez-Enriquez et al, 2011). Another example was recently provided by Kim et al (2009b). A histone acetyltransferase, GCN5 (for general control nonrepressed protein 5), was indicated to interfere with miRNA biogenesis transcriptionally and posttranscriptionally.…”

Section: Transcriptional Control Of Mirna Genesmentioning

confidence: 99%

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The Regulatory Activities of Plant MicroRNAs: A More Dynamic Perspective

et al. 2011

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Twenty years have elapsed since the discovery of a microRNA (miRNA) gene in Caenorhabditis elegans. Based on growing research progress, we are approaching the nature of this small RNA species, which seemed to be mysterious before. The regulatory activities of miRNAs have been extensively studied through target identification and physiological and phenotypic assays by using bioinformatic, genetic, and biochemical approaches. However, recent evidence points to the fact that the effective levels of miRNAs are determined by transcription, processing, miRNAinduced silencing complex loading, action, turnover use, and decay. Each process is affected by certain factors, such as genomic modifications, RNA editing, miRNA-induced silencing complex loading competition, target abundance and complementarity, and spatiotemporal effects, thus conferring a highly dynamic feature to miRNA activities. To maintain steady-state levels of the functional miRNAs, thus ensuring a normal physiological and biochemical status, plants employ several exquisite strategies, such as feedback regulation and a buffering system, to minimize the influence of external signal fluctuations. In this review, we raise the notion that a more dynamic picture of miRNA activities should be drawn to construct comprehensive miRNA-mediated networks in plants.MicroRNAs (miRNAs), approximately 21 nucleotides in length, were identified as a small RNA (sRNA) species with essential regulatory roles in various biological processes (Carrington and Ambros, 2003). The transcription of most miRNA genes is guided by RNA polymerase II Xie et al., 2005). Following transcription, the single-stranded RNAs with internal stem-loop structures are then recognized by Drosha and Dicer in animals (Kim et al., 2009a) or Dicer-Like1 (DCL1) in plants (Voinnet, 2009) for sequential cleavage, converting the primary microRNAs (pri-miRNAs) to the precursor microRNAs (pre-miRNAs) and finally to the miRNA/miRNA* duplexes. After dissociation from the duplexes, the miRNAs are incorporated into Argonaute (AGO)-associated micro-RNA-induced silencing complexes (miRISCs; preferentially AGO1-associated miRISCs). Although the sophisticated model of miRNA biogenesis is seemingly settled for each step, there exist many key nodes that influence the final activity of a miRNA gene. The transcription of miRNA genes is under the rigorous surveillance of many cis-and trans-factors, such as chromatin marks and specific transcription factors (TFs). The processing efficiency of the miRNA precursors is basically determined by their own sequences and structures and is regulated in a spatiotemporal manner (Davis and Hata, 2009;Cuperus et al., 2011;Zhu et al., 2011). Furthermore, the sorting of miRNAs into specific AGO complexes should not be oversimplified, since not all the miRNAs are uniformly loaded into AGO1-associated miRISCs. Additionally, loading competition between miRNAs and other sRNAs occurs in planta.In plants, miRNAs guide the miRISCs to target transcripts containing highly complementary recognition ...

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Section: Transcriptional Control Of Mirna Genesmentioning

confidence: 99%

“…A histone acetyltransferase, GCN5 (for general control nonrepressed protein 5), was indicated to interfere with miRNA biogenesis transcriptionally and posttranscriptionally. The GCN5-mediated histone modifications serve as an epigenetic mechanism for modulating miRNA production (Kim et al, 2009b).…”

Section: Transcriptional Control Of Mirna Genesmentioning

confidence: 99%

The Regulatory Activities of Plant MicroRNAs: A More Dynamic Perspective

et al. 2011

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“…For example, HYL1 activity is controlled by phosphorylation and dephosphorylation (30,31). Furthermore, the expression levels of the DCL1 complex are regulated at transcriptional and posttranscriptional levels to influence miRNA accumulation (32)(33)(34). The structure and length of the stem-loop also impact pri-miRNA processing.…”

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confidence: 99%

STV1, a ribosomal protein, binds primary microRNA transcripts to promote their interaction with the processing complex in Arabidopsis

Liu

Zhang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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MicroRNAs (miRNAs) are key regulators of gene expression. They are processed from primary miRNA transcripts (pri-miRNAs), most of which are transcribed by DNA-dependent polymerase II (Pol II). miRNA levels are precisely controlled to maintain various biological processes. Here, we report that SHORT VALVE 1 (STV1), a conserved ribosomal protein, acts in miRNA biogenesis in Arabidopsis. A portion of STV1 localizes in the nucleus and binds pri-miRNAs. Using primiR172b as a reporter, we show that STV1 binds the stem-loop flanked by a short 5′ arm within pri-miRNAs. Lack of STV1 reduces the association of pri-miRNAs with their processing complex. These data suggest that STV1 promotes miRNA biogenesis through facilitating the recruitment of pri-miRNAs to their processing complex. Furthermore, we show that STV1 indirectly involves in the occupancy of Pol II at the promoters of miRNA coding genes (MIR) and influences MIR promoter activities. Based on these results, we propose that STV1 refines the accumulation of miRNAs through its combined effects on pri-miRNA processing and transcription. This study uncovers an extraribosomal function of STV1.

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“…Stabilized1 (STA1), an Arabidopsis pre-mRNA processing factor, is shown to promote the expression of DCL1 [87]. Histone acetyltransferase GCN5 shows a general repressive effect on miRNA production via inhibiting the transcription of HYL1 and SE [88]. In addition, the transcript levels of DCL1 can be post-transcriptionally regulated.…”

Section: Regulation Of the Levels Of Dcl1 And Hyl1mentioning

confidence: 99%

microRNA biogenesis, degradation and activity in plants

Xie

Zhang

2014

Cell. Mol. Life Sci.

115

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microRNAs (miRNAs) are important regulators of gene expression. After excised from primary miRNA transcript by dicer-like1 (DCL1, an RNAse III enzyme), miRNAs bind and guide their effector protein named argonaute 1 (AGO1) to silence the expression of target RNAs containing their complementary sequences in plants. miRNA levels and activities are tightly controlled to ensure their functions in various biological processes such as development, metabolism and responses to abiotic and biotic stresses. Studies have identified many factors that involve in miRNA accumulation and activities. Characterization of these factors in turn greatly improves our understanding of the processes related to miRNAs. Here, we review recent progress of mechanisms underlying miRNA expression and functions in plants.

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Histone acetyltransferase GCN5 interferes with the miRNA pathway in Arabidopsis

Cited by 75 publications

References 58 publications

The Regulatory Activities of Plant MicroRNAs: A More Dynamic Perspective

The Regulatory Activities of Plant MicroRNAs: A More Dynamic Perspective

STV1, a ribosomal protein, binds primary microRNA transcripts to promote their interaction with the processing complex in Arabidopsis

microRNA biogenesis, degradation and activity in plants

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