2023
DOI: 10.1186/s12985-023-02128-6
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Histone deacetylase III interactions with BK polyomavirus large tumor antigen may affect protein stability

Abstract: Background Human polyomavirus BK (BKPyV) causes associated nephropathy and contributes to urinary tract cancer development in renal transplant recipients. Large tumor antigen (LT) is an early protein essential in the polyomavirus life cycle. Protein acetylation plays a critical role in regulating protein stability, so this study investigated the acetylation of the BKPyV LT protein. Methods The BKPyV LT nucleotide was synthesized, and the protein wa… Show more

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“…The immunoprecipitates were separated by SDS-PAGE, and the gel was stained with PageBlue Protein Staining Solution (ThermoFisher, Rockford, IL, USA) to visualize the protein bands. The band of interest was excised from the gel and subjected to in-gel digestion as described previously [ 36 ]. The resulting peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an Orbitrap Fusion mass spectrometer (ThermoFisher) equipped with an EASY-nLC 1200 system (ThermoFisher) and an EASY-Spray HPLC column (75 μm × 150 mm, 3 μm, 100 Å).…”
Section: Methodsmentioning
confidence: 99%
“…The immunoprecipitates were separated by SDS-PAGE, and the gel was stained with PageBlue Protein Staining Solution (ThermoFisher, Rockford, IL, USA) to visualize the protein bands. The band of interest was excised from the gel and subjected to in-gel digestion as described previously [ 36 ]. The resulting peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an Orbitrap Fusion mass spectrometer (ThermoFisher) equipped with an EASY-nLC 1200 system (ThermoFisher) and an EASY-Spray HPLC column (75 μm × 150 mm, 3 μm, 100 Å).…”
Section: Methodsmentioning
confidence: 99%