Histone H5 is a potent Antimicrobial Agent and a template for novel Antimicrobial Peptides

Jodoin, Joelle; Hincke, Maxwell T.

doi:10.1038/s41598-018-20912-1

Cited by 35 publications

(19 citation statements)

References 57 publications

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“…Microbes in biofilms are more resistant to antibiotics than freely suspended planktonic forms. The major biofilm forming pathogens are Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa (Jodoin and Hincke 2018). Total histones (H1, H2A, H2B, H3, H4 and H5) from chicken erythrocytes were found to exhibit antibiofilm activity (Rose-Martel and Hincke 2014).…”

Section: Discussionmentioning

confidence: 99%

A histone H2A-derived antimicrobial peptide, Hipposin from mangrove whip ray, Himantura walga: Molecular and functional characterisation

et al. 2020

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Antimicrobial peptides (AMPs) are biologically dynamic molecules produced by all type of organisms as a fundamental component of their innate immune system. The present study deals with the identification of a histone H2A-derived antimicrobial peptide, Hipposin from mangrove whip ray, Himantura walga. A 243 base pair fragment encoding 81 amino acid residues amplified from complementary DNA was identified as Hipposin and termed as Hw-Hip. Homologous analysis showed that Hw-Hip belongs to the Histone H2A superfamily and shares sequence identity with other histone-derived AMPs from fishes. Phylogenetic analysis of Hw-Hip displayed clustering with the fish H2A histones. Secondary structure analysis showed the presence of three α-helices and four random coils with a prominent proline hinge. The physicochemical properties of Hw-Hip are in agreement with the properties of antimicrobial peptides. A 39-mer active peptide sequence was released by proteolytic cleavage in silico. Functional characterisation of active peptide in silico revealed antibacterial, anticancer and antibiofilm activities making Hw-Hip a promising candidate for further exploration.

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Section: Discussionmentioning

confidence: 99%

A histone H2A-derived antimicrobial peptide, Hipposin from mangrove whip ray, Himantura walga: Molecular and functional characterisation

et al. 2020

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“…Cationic AMPs are said to be positively correlated with increased anti-microbial activities ( Table 5). Therefore all the anti-bacterial AMPs which were positively charged indicated conformity with ideal AMPs with improved antimicrobial activities [37]. Isoelectric potential (pI) of peptides is a function of individual amino acids in both backbone groups.…”

Section: Discussionmentioning

confidence: 99%

Identification of biomarkers for the accurate and sensitive diagnosis of three bacterial pneumonia pathogens using in silico approaches

Bakare

Keyster

Pretorius

2020

BMC Mol and Cell Biol

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Background Pneumonia ranks as one of the main infectious sources of mortality among kids under 5 years of age, killing 2500 a day; late research has additionally demonstrated that mortality is higher in the elderly. A few biomarkers, which up to this point have been distinguished for its determination lack specificity, as these biomarkers fail to build up a differentiation between pneumonia and other related diseases, for example, pulmonary tuberculosis and Human Immunodeficiency Infection (HIV). There is an inclusive global consensus of an improved comprehension of the utilization of new biomarkers, which are delivered in light of pneumonia infection for precision identification to defeat these previously mentioned constraints. Antimicrobial peptides (AMPs) have been demonstrated to be promising remedial specialists against numerous illnesses. This research work sought to identify AMPs as biomarkers for three bacterial pneumonia pathogens such as Streptococcus pneumoniae, Klebsiella pneumoniae, Acinetobacter baumannii using in silico technology. Hidden Markov Models (HMMER) was used to identify putative anti-bacterial pneumonia AMPs against the identified receptor proteins of Streptococcus pneumoniae, Klebsiella pneumoniae, and Acinetobacter baumannii. The physicochemical parameters of these putative AMPs were computed and their 3-D structures were predicted using I-TASSER. These AMPs were subsequently subjected to docking interaction analysis against the identified bacterial pneumonia pathogen proteins using PATCHDOCK. Results The in silico results showed 18 antibacterial AMPs which were ranked based on their E values with significant physicochemical parameters in conformity with known experimentally validated AMPs. The AMPs also bound the pneumonia receptors of their respective pathogens sensitively at the extracellular regions. Conclusions The propensity of these AMPs to bind pneumonia pathogens proteins justifies that they would be potential applicant biomarkers for the recognizable detection of these bacterial pathogens in a point-of-care POC pneumonia diagnostics. The high sensitivity, accuracy, and specificity of the AMPs likewise justify the utilization of HMMER in the design and discovery of AMPs for disease diagnostics and therapeutics.

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“…Furthermore, antimicrobial activity for the full-length histones in all classes (H1, H2A, H2B, H3, and H4) [40] and more recently for the histone H5 from chicken erythrocytes [41,106] has been reported. However, the fragmented histones only displayed antimicrobial action for the classes H1, H2A, and H4 [40].…”

Section: Are Amps and Histones Part Of The Host Defense Barrier In Thmentioning

confidence: 98%

“…CAMPs and histones, mostly made up of basic and hydrophobic amino acids, are deemed to form amphiphilic three-dimensional structures able to interact with cell membranes [41]. A high number of them were detected/clustered together in the same proteinGroup by the MaxQuant (Tables S1 and S6).…”

Section: Are Amps and Histones Part Of The Host Defense Barrier In Thmentioning

confidence: 99%

Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database

et al. 2020

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Cephalopods, successful predators, can use a mixture of substances to subdue their prey, becoming interesting sources of bioactive compounds. In addition to neurotoxins and enzymes, the presence of antimicrobial compounds has been reported. Recently, the transcriptome and the whole proteome of the Octopus vulgaris salivary apparatus were released, but the role of some compounds—e.g., histones, antimicrobial peptides (AMPs), and toxins—remains unclear. Herein, we profiled the proteome of the posterior salivary glands (PSGs) of O. vulgaris using two sample preparation protocols combined with a shotgun-proteomics approach. Protein identification was performed against a composite database comprising data from the UniProtKB, all transcriptomes available from the cephalopods’ PSGs, and a comprehensive non-redundant AMPs database. Out of the 10,075 proteins clustered in 1868 protein groups, 90 clusters corresponded to venom protein toxin families. Additionally, we detected putative AMPs clustered with histones previously found as abundant proteins in the saliva of O. vulgaris. Some of these histones, such as H2A and H2B, are involved in systemic inflammatory responses and their antimicrobial effects have been demonstrated. These results not only confirm the production of enzymes and toxins by the O. vulgaris PSGs but also suggest their involvement in the first line of defense against microbes.

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Histone H5 is a potent Antimicrobial Agent and a template for novel Antimicrobial Peptides

Cited by 35 publications

References 57 publications

A histone H2A-derived antimicrobial peptide, Hipposin from mangrove whip ray, Himantura walga: Molecular and functional characterisation

A histone H2A-derived antimicrobial peptide, Hipposin from mangrove whip ray, Himantura walga: Molecular and functional characterisation

Identification of biomarkers for the accurate and sensitive diagnosis of three bacterial pneumonia pathogens using in silico approaches

Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database

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